Protein
Modification methylase ScrFIA
Organism
Lactococcus lactis subsp. cremoris
Function
This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
Sequence
MTTISRNTGTEISIMIKEKRLRLNMTQKELADAVGMSKNGDRTIRRWENGETCPSQLEISAILRFPEIAPFENRKTAKYKMIDLFAGIGGTRLGFHQTEKVKSVFSSEIDKFAIKTYKANFGDEPHGDITKIDEKDIPDHDILVGGFPCQAFSQAGKKLGFDDTRGTLFFEIARIIKEKRPKAFLLENVKNLKTHDKGRTFKTILNTLEELDYEVHTALFKARDFGLPQNRERIYIVGFDRKSISNYSDFQMPTPLQEKTRVGNILESVVDDKYTISDKLWDGHQRRKTENKKNGKGFGYTLFNQDSEYTNTLSARYYKDGSEILIEQKNKNPRKITPREAARLQGFPENFIIPVSDTQAYKEFGNSVAVPTIHAIAEKMLEVLEKSKK