Protein
L-serine kinase SbnI
Organism
Staphylococcus aureus (strain NCTC 8325)
Function
Free serine kinase that uses ATP to phosphorylate L-serine to yield O-phospho-L-serine and ADP. O-phospho-L-serine serves as a substrate for SbnA and is a precursor for staphyloferrin B biosynthesis (PubMed:29483190). Is also a DNA-binding regulatory protein that senses heme to control gene expression for siderophore biosynthesis. Binds to DNA within the sbnC coding region and is required for expression of genes in the sbn operon from sbnD onward (PubMed:26534960).
Sequence
MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPILVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETLQQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVERIAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLALLKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ