Protein
Crossover junction endodeoxyribonuclease RuvC
Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Function
Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.
Similarity
Belongs to the RuvC family.
Sequence
MNHEGLRVMGIDPGLTRCGLSVVQAGRGRTVYPVSVGVVRTPPDAELAERLLRLSKAVGEWMDEYTPDVIAIERVFERGNVSTVMNTAHAVGVLILAAAERGLPVHMYTPSEVKKAISGNGRADKKQMTVMITRILGLGEPPKPADAADALSLAVCHCWRAPMLMRAQSQYSEQELEKRRRVQQGKLGKAKSTYNAEQAQSHASDPAKAAHPSQFQRTDTN