Protein
Recombination-promoting nuclease RpnB
Organism
Escherichia coli (strain K12)
Function
A low activity DNA endonuclease yielding 3'-hydroxyl ends (Probable). Upon expression enhances RecA-independent DNA recombination 19-fold, concomitantly reducing viability by 98% and inducing DNA damage as measured by induction of the SOS repair response.
Similarity
Belongs to the Rpn/YhgA-like nuclease family.
Sequence
MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTTLKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAAMQNHLDAGYKELPLVLPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH