Protein
Recombination-promoting nuclease RpnA
Organism
Escherichia coli (strain K12)
Function
A low activity DNA endonuclease yielding 3'-hydroxyl ends, equally active on ss or dsDNA, not active on dsRNA (PubMed:28096446). Shows no sequence specificity (PubMed:28096446). Upon expression enhances RecA-independent DNA recombination 49-fold, concomitantly reducing viability by 88% and probably inducing DNA damage as measured by induction of the SOS repair response in RecA cells (PubMed:28096446). RecA-independent DNA recombination leads to replacement of recipient genes with large segments of donor DNA rather than DNA addition to the donor strain; increased expression of RpnA leads to smaller replacement segments, suggesting this protein may play a role in generating crossover events (PubMed:28096446).
Similarity
Belongs to the Rpn/YhgA-like nuclease family.
Sequence
MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKTLKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAAMQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVTVMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGLSEEELAAASQ