Function
Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis (PubMed:11590237, PubMed:18765790). Required for chromosome segregation, the assembly of the dynein-dynactin and mdf-1-mdf-2 complexes onto kinetochores and spindle pole separation (PubMed:18765790, PubMed:19109417). Plays a role in nuclear envelope breakdown (PubMed:19109417). Its function related to the spindle assembly machinery and kinetochore-microtubule attachments likely depends on its association in the mitotic RZZ complex (PubMed:18765790, PubMed:24231804). The RZZ complex recruits the spindly-like protein spdl-1 to kinetochores (PubMed:18765790, PubMed:24231804). To prevent irregular chromosome segregation, the complex also inhibits the attachment of the kinetochore-associated NDC80 complex to microtubules (PubMed:24231804). The recruitment of spdl-1 to kinetochores relieves this inhibition (PubMed:24231804). Required for embryonic development (PubMed:11590237, PubMed:18765790, PubMed:19109417).
Sequence
MGRLGEAKAVDLEAETSTVGLYDVCTVGRILPLLNEMSIDKKSPAWKLKPKSNERFLVLASCSDLAVFSLGEKSDYSFNTGLGGAITDFEILGSSSFFVGVIEHRRIVILSLDKQEIYLNEPAPHEIDFVVCHTTSSVCQLIFGSRSDNWHTITLPGDVAASKETNRFENWHRDQIQEFFHQAMGKTVSKSVDMSKVAGDITLISNGPLQTFASIDQQRGIHWCGLVESEFEVIGMEKQFLQVRDGGRFSLHLDTDGWIHVFDSLVSSFCHEFDMKMSPDDKILDFVIIDKNEVEVPKMFAILVLPRDGDSTKMMIYDRLNKDNCFALDSSTSTTLFAYGGSDRVLIAVEELENSLADEQDGSQIVVRQVSQSRPEMRFESLLKNNRFEEAEKFAMAFMLDVQKVYKGHVHYLMDSCDESDESFETLMTKMGQIQDHNMVAETYFTLVGMSRRCDRIRTYLTHAKKRRITDLDILKMIEALCYTWGTYRIIAGPEENEPSRPQVNETIWDLFVEALHDANPWIEIYNEFISDAQFTQARVIFSRHGKSITDYMCDDEENTISRLETLFRMFIDAISSDISKWSNVIEHVTTDILPACVMITEELIPCLESLITSLISLLEYRDSTNWPENAIKAASSYDTMTKILSNNGNTPATQCILVMYGSKLGSSAGNKPSSMSRIKKIYYDLIELKRLKDVYECSISFSVFQNMSSEQICHKILQNALANPNMTHAKIEKFVKPFMAERHLDQEQTIVNYIQMMSGAAVTNANLFGWEKQCVQLCASLMDETRRCCSIISIASTAKIPWPAELNEAVEKILASRTLLRSEIEQMHLVCKRTELYKMLSSYGFSRQDIELLTTPDSNMDIILTIRCMLAHREKASRFVDVIKLVDLLKAMQGSSQPRTLRIEYVQSFAIIHMMSHQDVTISIINYIDSLGDRERVKTISLVFSFIECVANAPATGDNVLEREKILGVGEELMSHYTCRDNNFNDPERRLKDELVLLREVQKTETKAVLLSELKDEDWQRRFLERLIESNSSMSLNLGRCSYMGISPEHLIEMILTKATVENDMDTIVDSITNYVEFINSLTDSSREMLEPIVQILSWITFRLPKLLPNETEMARADYIAFVVKRIGRVVRETLRRFSFDVISDDLDYLLQLEAFYHLGEHIIKQSLRGQENENEKQEMFRSDDDTFLPTDSSNPFSNQSSLRIFEFKRPLGTFDFSNDPALFEGVQGVLALAMVAPSVARPYDSEISPDDANEFRSSWEQLNMFLAMHSQDLLDISARVFAGSLKCWAGEYLQGIVEMEQPILSVVERMLQQKKFDFWHAVTLLGGIPLERLDRAIIDLQKRQGVRSSTKATIQYLQLAFVMSLLARNMEKVPTIVSAYEQKYLVKKLAEEGIRVSITQDFVDKVLQQAIDLRQPLSPLRLHDYVKKYVEKLIRNSKISVGEYMVRYATLLIRKASVAGRHTDREIRKEEIEKYIEAARIALRIAEEEDASCICNYLHCLLYVVCPYNYEVIQFIVTSYGKYATETVEIEFNKNLKSIMAFLWAYQRTNHISNEESIWFTKRESVLMKDEKEFEKTGRMLDPFGHSLRVVYEDDGSNMSDSYNSDLALSSDAMVYERNSVIISDLPSLAEQYLPFHAFLLRKKEEIDEIVMGIVKAELSIFNVPIWQTFLREVSWLSSRFSRSQLLSSAIFAHANKYAKFGKSLPDGERNVIYELLNSASQRHVVVSTIALLFKRIILSDVKIELLQMGVNISERWTSDLTGEEQQEMEEQSARLKDGIAKFSTELELKKNGLYNEKTADNIENVSELCSLIYNEMVQWDDSRDVLKKCQVVDKIAKANGLDLTALHEQLVFSWVEDTQTIISINHVDMNESIGGTSFLDHKDETDDQNDLRIPLFDGILDKVVVLCQRIDKKRLLTRLGSILMRGGRKATGGYTAVVRATCIILRSFTDTEVSELLSGADMFALCSTLENQLYERLFEKAEVKGDCKTDKMQLIKSLLQCPSRTHPMTALIACLIIDHEFKDPKRDISLGWDVGCVPVSANSSNS