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ptsJ

Gene
ptsJ
Protein
Vitamin B6 salvage pathway transcriptional repressor PtsJ
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Length
430 amino acids
Function
Acts as transcriptional repressor of the pdxK gene, encoding a pyridoxal kinase involved in the vitamin B6 salvage pathway. Also represses transcription of its own gene. Binds to the ptsJ-pdxK intergenic region, but does not bind pdxY and pdxH promoters. Among all six B6 vitamers, only pyridoxal 5'-phosphate (PLP) clearly binds to the protein and acts as an effector molecule for PtsJ, inducing a protein conformational change that increases affinity for DNA. Thus, PLP stabilizes protein-DNA interactions, reinforcing repression.
Similarity
In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Mass
46.508 kDa
Sequence
MIDGKTANEIFDSIRQHIIAGTLRAEDSLPPVRELASELKVNRNTVAAAYKRLITAGLAQSLGRNGTVIKGSPSPVALEGGDPHTPLHDLSGGNPDPQRLPDLSRYFARLSRTPHLYGDAPVSPELHAWAARWLRDATPVAGEIDITSGAIDAIERLLCAHLLPGDSVAVEDPCFLSSINMLRYAGFSASPVSVDSEGMQPEKLERALNQGARAVILTPRAHNPTGCSLSARRAAALQNMLARYPQVVVIIDDHFALLSSSPWQPVIAQTTQHWAVIRSVSKTLGPDLRLAIVASDSATSAKLRLRLNAGSQWVSHLLQDLVYACLTDPEYQHRLTQTRLFYAARQQKLARALQQYGIAISPGDGVNAWLPLDTHSQATAFTLAKSGWLVREGEAFGVSAPSHGLRITLSTLNDSEINTLAADIHQALNR