Protein
Modification methylase PspPI
Organism
Psychrobacter sp. (strain TA137)
Function
This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the PspPI endonuclease.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
Sequence
MKFPDNHFSAAIVADTLSVSKRNVETWTQNGKLVPALDPNIHEKPYTKDQLETFEQFSAMFNSSWCEEMAVEPSRSYSLVELFAGAGGLALGLEQAGFKSVLLNEKDKYACATLRANRPNWNVIEDDIENVDFTHLNGKVDLLTGGFPCQPFSYAGKQLGFEDLRGTLVFEMARAIKEIKPKVFLAENVKGLAENDGGRTLSIIIKVLEDLGYKILEKEVYKAIFYKVPQKRERLIIIGVRTDLYDKLAYEKPSPYYKVLTVADALKAGELYDVDVPESTGQLYPERKAEIMSYVPEGGYWRDLPIRIAKEYMMKSFYLGGGKTGMARRLSWDEPSLTLVCTPAQKQTERCHPSESRPLTTREYARIQTFPDDWEFKGSVGQIYKQIGNAVPVNLALAIGKAIIRMLNAAPKDVFE