Organism
Dictyostelium discoideum
Function
Plays a role in cell growth. Hydrolyzes membrane phospholipids, such as PtdCho (phosphatidylcholine), producing the free headgroup and PtdOH (phosphatidic acid; signaling molecule on its own). Involved in the inhibition of actin-based motility and endocytosis. Its inhibition causes complete collapse of F-actin organization. Plays an important role in cell migration by localizing along the anterior cell membrane. Overexpression leads to the inability to aggregate even at higher cell density. Also known as a negative regulator of quorum sensing.
Similarity
Belongs to the phospholipase D family.
Sequence
MNLSQEHAINQNLHKNQKNEEKIEKKTINKDGRGQMNYDGEEGQGEKSRFQKMVENEKIEEPQQKDENIPNTDVIERKEVGVIERINSEDVPILMTDNATDYKVLQHNLKKGKVSKKSKEQIEQISKQEENDMLEYLKNYKSNEEFTIQLEDLLSFETYFSRDELHLLYREFKTISKSGMFMSKEDFISKLTPFSRNADLTISLMNAIDRNGDQKIAFPEFVQALSIMCRGTKKERLRFTFEICDFNGDSLVSRDEVYSTVKAISDIFSKFGYSKDKFGDPSEAVDSIFSSGLTTNGIYLHNKKELTLNEFLERGELNPDLSKCFGMFDYFYLKFIGQIDLLFKDKEINMNGQLTKIKPKTIFNFNISHRRTLSLRDGFLIVYKKKKFKHDEDKPSKVIFLPGSTVKVVVGSQPSKKKKFLSKKFHNYYGFRVTKGNYNRFFLMENRDEALNWVNAIRFHSRQGFRFQSFSKVRSNISVEWFINGSSYYNELAETIRRAKHEIFITGWWVSPYVYLQRDNGIENMEKSRLDRILTEKAKEGVKVYVLMWNETNLGVQLGSRHAKNWLEGCHSNIHVIRHPKRYPLSWSHHQKNAIIDQQIAFVGGIDICLMRYETSKFQLTDDQGKRFPGKDYGNLLGTVIRTGDPKKDQFNRRECPRMPWHDVHTKIVGPSAKDVASNFIQRWNHAIYVERSNRFQPILVPKNYTGLPSDDAKPDKWKNLVSNIRKGFSHVSYGREKPTHYQRAGDNPKVRSHTRQGAFGLQSDQIDNKIDKQKNNSTNSENSENSYSEFDEEDEEGNQEEEDEDEFDEFEKDENQKQENSKIPFNNKPSDAGGLKSKNYKNNNNNNIIESLKDEESFELPGTPKIDLNNDKLLKSIYHLSSNMSENSCVVQMVRSICPWSAGTDVEDSCYKAYLGLIKNAQHFIYIQNLFFISSCGSKLPKNRIALAILNRVRRAITLKEKFRVIIMVPISPSGDLALASSRMIIGWTNRTISQGGQSILELLKNEFPDVDLDQYISFNSIRQWEANGDRIFTEQIYVHSKVLIVDDRVAVIGSCNINDRSMMGSRDSELAVVVSDQSKLLITMNGKPFKVGKFPHTLRVGLWKTHLNLTDSEISSIIDPITDNAFINIWRKTARNNSIIYKEVFGDCILENQRRLGIVQKKYIPKTNELIVQLSQIQGVLIEYPLDMFCESNLFNEQVGIFTAESYVDVSIFT