PHF8

Histone lysine demethylase PHF8

1060 amino acids

Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.

Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.

117.864 kDa

MNRSRAIVQRGRVLPPPAPLDTTNLAGRRTLQGRAKMASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSKGHDTHKGKPVKTGSPTFVRELRSRTFDSSDEVILKPTGNQLTVEFLEENSFSVPILVLKKDGLGMTLPSPSFTVRDVEHYVGSDKEIDVIDVTRQADCKMKLGDFVKYYYSGKREKVLNVISLEFSDTRLSNLVETPKIVRKLSWVENLWPEECVFERPNVQKYCLMSVRDSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFECWSSSSNQNEMFFGDQVDKCYKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIEKRLSTADLFRFPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTRKEALPDHEDEIPETVRTVQLIKDLAREIRLVEDIFQQNVGKTSNIFGLQRIFPAGSIPLTRPAHSTSVSMSRLSLPSKNGSKKKGLKPKELFKKAERKGKESSALGPAGQLSYNLMDTYSHQALKTGSFQKAKFNITGACLNDSDDDSPDLDLDGNESPLALLMSNGSTKRVKSLSKSRRTKIAKKVDKARLMAEQVMEDEFDLDSDDELQIDERLGKEKATLIIRPKFPRKLPRAKPCSDPNRVREPGEVEFDIEEDYTTDEDMVEGVEGKLGNGSGAGGILDLLKASRQVGGPDYAALTEAPASPSTQEAIQGMLCMANLQSSSSSPATSSLQAWWTGGQDRSSGSSSSGLGTVSNSPASQRTPGKRPIKRPAYWRTESEEEEENASLDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGTRVASIETGLAAAAAKLAQQELQKAQKKKYIKKKPLLKEVEQPRPQDSNLSLTVPAPTVAATPQLVTSSSPLPPPEPKQEALSGSLADHEYTARPNAFGMAQANRSTTPMAPGVFLTQRRPSVGSQSNQAGQGKRPKKGLATAKQRLGRILKIHRNGKLLL

phf8

Histone lysine demethylase PHF8

1032 amino acids

Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate (By similarity). Required for brain development, probably by regulating expression of neuron-specific genes.

Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.

114.222 kDa

MASVPVYCLCRLPYDVTRFMIECDVCQDWFHGSCVGVEEDKAAEIDLYHCPNCQVTHGPSVMRKRRGAVKHADVGLGRDSGRPVKTGSAQFVRELRCRTFPSADEVLLKPTGAQLTVEFLEERSFSVPVLVLRKDGLGMNLPPSSFSVTDVEHYIGTEKEIDVIDVSRQADLKMKLGEFVEYYNSPNRDRVLNVISLEFSDTRLSNLVETPKIVRKLSWVENLWPEESIFERPNVQKYCLMGVKDSYTDFHIDFGGTSVWYHVLRGEKIFYLIRPTAANLSLFERWSSSSNQNELFFGDQVDMCYKCSVKQGNTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIDMQLRAYEIEKRLSTADLFKFPNFETVCWYVGKHLLDTFRGLRENRRHPATYLVHGAKALNNAFRGWTRKESLGEHEQEIPDTIKTQQLVKDLAKEIRLVEDIFQQNIGRSGTPFGGSQGLPSPHPKAQLNTPLTFSQHLSKKRGPKPKEAFGGGGVGPPGAKKKSQKGKEIKTEAGELDLLEIHTKHTLKKFQPGCKVKKSKLELPDDCLDDFEEKINKSKLKLVLTNGKLQGKKGRAGSANGAGSSLQQFQPHMATLSDFDSEDELQIDETPPPRRRPSLPSKKKLAGLPRKLPRAKPCSDPHRIREPGEVDFDIEEDYTTDEEMLTMQGVKGGAGGILDLLKASKQVAGLDSALSEEAPASPSTRDAIQGMLSMANPPSSSSSSSSSSPLSISGGGEMMGLMKEKGGREGWMSGVKKSERKAVFQRPGKRPIKRPARHLSDDESLDEQETLGTCFKDSDYVYPSLESDEEDHVSKSKMKRKRNWDDTPWSPKARVTPTLPKQERPVREGARVASVETGLAAAAAKLAQQEQQKTITKRKYTKKKTPQEKVHSTVAQLQHQPDSAPVSPPLPSEPPVDCIVEERRVEVYSASLLDHEYTAGPGPFSPGGPRGSGAMAPGVFLTSRRPSLSPQNSSSYSPSAPSPGGLVTPTSAGACQGKRPKKGLATAKQRLGKILKIHRNGKLLL

Phf8

Histone lysine demethylase PHF8

1023 amino acids

Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.

Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.

113.553 kDa

MASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSKGHDNHKGKPLKTGSSMFIRELRGRTFDSSDEVILKPTGSQLTVEFLEENSFSVPILVLKKDGLGMTLPSPSFTVRDVEHYVGSDKEIDVIDVARQADCKMKLGDFVKYYYSGKREKVLNVISLEFSDTRLSNLVETPRIVRKLSWVENLWPEECVFERPNVQKYCLMSVRDSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFECWSSSSNQNEMFFGDQVEKCYKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIEKRLSTADLFKFPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTKKEALPDHEDEIPETVRTVQLIKDLAREIRLVEDIFQQNVGKTSNIFGLQRIFPAGSIPLTKPAHSTSVSMSKLSLPSKNGSKKKGLKPKDIFKKAERKGKQSSALGPAGQLSYNLMDPYSHQALKTGPSQKAKFNMSGTSLNDSDDDSADMDLDGSENPLALLMANGSTKRMKSVSKSRRAKIAKKVDSARLVAEQVMGDEFDLDSDDELQIDERLGKEKANLLIRSKFPRKLPRAKPCSDPNRIREPGEVEFDIEEDYTTDEDMVEGVESKLGNGSGAGGILDLLKASRQVGGPDYAALTEAPASPSTQEAIQGMLCMANLQSSSSSPATSSLQAWWTGGQERSSGSSSSGLGTVSSSPASQRTPGKRPIKRPAYWKNESEEEENASLDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGTRVASIETGLAAAAAKLAQQELQKAQKKKYIKKKPLLKEVEQPRPQDSNPIMTMPAPTVATTPQPDTSSSPQPPPEPKQEALSGSLADHEYTARPNAFGMAQANRSTTPMAPGVFLTQRRPSVGSQSSQAGQGKRPKKGLATAKQRLGRILKIHRNGKLLL