Protein
rRNA 2'-O-methyltransferase fibrillarin
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Function
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity).
Similarity
Belongs to the methyltransferase superfamily. Fibrillarin family.
Sequence
MGFERGGRGGARGGGRGGFGGDRGGRGGARGGSRGGFGGGRGGGAPRGRGGPRGGGRGGATRGRGGARGGAKGGAAGKKVIVEPHRHKGVFVARGGKEDLLATVNLVPGESVYGEKRISVENASKEEGGASTKTEYRIWNPFRSKLAAGILGGLETIYMKPGSKVLYLGAASGTSVSHVADIVGPTGSVYAVEFSHRSGRDLINMATRRTNVIPIVEDARKPMAYRMLLPMVDVIFADVAQPDQARIVGINAKLFLKQGGGLLISIKASCIDSTAPPEQVFASEVQKLREDKFFPKEQLTLEPYERDHAMVSCVYQQKEFVDN