neoN

Neamine transaminase NeoN

416 amino acids

6'-oxoglucosaminyl:L-glutamate aminotransferase that catalyzes pyridoxal-5'-phosphate-mediated transamination for the conversion of paromamine to neamine in the biosynthetic pathway of neomycin. Also able to catalyze deamination at C-6''' of neomycin.

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

44.643 kDa

MTKNSSLLAEFPTCPRDEKDRPRVFTAASGAWLTDESGFRWIDFDNARGSILLGHGDPVVAEAVARAATGADGTATGWSRRVDAVLERLHALCGGEVVGLFRSGTAAVRAAVLAVREATGRPLLLSAGYHGYDPMWYPSEAPLEPNADGVVDFFFDLGLLRELLRAPERVAAVVVSPDHMHLSPGWYRELRRLCSAAGVVLVADEVKVGLRYAPGLSTAELLAPDVWVVAKGMANGHAVSAVGGSRRLLKPLKEVSFTSFFEPTILAAADAALARVATGEPQRAVREAGDRFLRHARKALDDASLPVEIAGDGTFFQFVPATEELEEALYGAANAEGLLFYAGDNQGVSAAFDEAVLGEAERRFARVCERLAPYAGGEPVGDAARYRVAWNVMDGLRQAPRDREETTGLLARLLDD

neoN

Neomycin C epimerase

299 amino acids

Catalyzes the last step of neomycin B biosynthesis, i.e. the irreversible epimerization at C-5''' of neomycin C to give neomycin B. To a lesser extent, is also able to convert neomycin Y2 to neomycin Y1.

Belongs to the radical SAM superfamily.

34.363 kDa

MTTDIVWPPPVRQVRAYRNIVVDGACNIRCTYCEVKKTKVDQPATIRSLDRIFAEYEPDAVLFRVESDGEITLYPKIVDHLQKRAAEGYRVEVLSNGTKLPRALEGRPDLLWVFSVDGHTEAMNAKRGLKQPQIDRILDAAVELGAELQTVYWGQPVEEVNAYIDLLESRGYRGLLHFMPLLAFKGRPLTVNLRYQDLHPADFLAPPEYFRRWNHIFETGRRDAVCDQITNGYNYQVSGDEIRMVKCDCYSVPKHLVHGFGPIREFDDWPCGTCIANQEFNNSRERMRVPQGRIPLPLV