Function
Involved in the caffeine degradation, which is the essential first step for assimilating the carbon and nitrogen in caffeine. Catalyzes the N3-demethylation of theobromine to produce 7-methylxanthine and formaldehyde. Also catalyzes the N3-demethylation of 3-methylxanthine, caffeine, and theophylline to xanthine, paraxanthine, and 1-methylxanthine, respectively. NADH is the preferred substrate.
Sequence
MKEQLKPLLEDKTYLRHFWHPVCTLNEFERANASGHGPMGVTLLGEKLVLARLNSKIIAAADRCAHRSAQLSIGRVCSNAGKDYLECPYHGWRYDEAGACQLIPACPDKSISPRAKISSFDCEVKYDIVWVRLDNSFDCTQIPYLSDFDNPDMQVIVADSYIWETVAERRWENFTDFSHFAFVHPGTLYDPFFASHPTVYVNRVDGELQFKLAPPREMKGIPPEAPMGDFTYRCTMPYSVNLEIKLWKDDSRFVLWTTASPVDNKSCRNFMIIVREKDNQPDHMHLAFQKRVLDEDQPVIESQWPLEIQTSEVSVATDKISVQFRKWHKELSLSAVEGREAFRDSVLTNVIEEEQ