Protein
2-hydroxy-5-methyl-1-naphthoate 7-hydroxylase
Organism
Streptomyces carzinostaticus
Function
Involved in the biosynthesis of the naphthoic acid (NA) moiety in the chromophore of the enedyine antitumor antibiotic neocarzinostatin (NCS). Catalyzes the hydroxylation at C-7 position of 2-hydroxy-5-methyl-1-naphthoate to yield 2,7-dihydroxy-5-methyl-1-naphthoate.
Similarity
Belongs to the cytochrome P450 family.
Sequence
MCPYRLDPEGADTHGETARLREQGPIARVELQDGVLAWSVHDYAVAKQIMADERFSKNPRKNWPAYINGEISNGWPLITWVAMDTMATQDGADHARLRKLLLKAFTERRVESMRPHIEKTVKELLDNMAAKADDEIVDIKEMFHAELPTRLMCDLFGVPEERRAEVLAGGHKNIDTRISSEAAEANLGQWQEAISDLVEYKRHHPGDDLTSALIEARDEGSRLSDSELIGTLHLLLGAGSETLVNALAHSSLALLVDADLRKKVTSGEIPWVNVWEETLRVESPVAHLPFRYATEDFEIGGVKISKGDPLLVDFAGIGRDPAVHSDAPDEFDALRPDKTHLSFGHGVHYCLGARLAKHAWMIGIPALFERFPDMELAVRRDELKGQGSFVVNGHASLPVHLKGRAAALAR