Protein
tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Function
Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34.
Similarity
In the C-terminal section; belongs to the DAO family.
Sequence
MSDFQHAQLDWDENGQPLSRAFGDVYFSRHSGLNETRHVFLATNRLAERFAALGDGEVLCIGETGFGTGLNFLCAWQLFERVAPAGARLEFVSVEKFPLAAADLRRALALWPELAPWSEALLGQYLAVHPGFQRLAFAGGRVGLTLLLGDALECLPQLDARVDAWFLDGFAPAKNPDMWSPVLFAELARLSAPQATLGTFTSAGFVRRGLVEAGFAMQRVPGYGQKREMLSGTYQGPPANAGKPWYARPAPHAGRRAALVVGGGLAGCASAASLAARGWQVTLIERHPGLAREASGNPQGVLYLKLSAHGTPLSRLVLSGFGHTRRLLERLRRGHDWDACGVLQLAFDAKEAQRQAQLAAAFPADLLHGLEREQAERLAGVALPAGGLFYPEAGWVHPPALCQALAATPGITLLSGRAVRLRREGDDWCAYAGDECLARAPLAILATAADIRDFPPAAELPLKRIRGQVTRLPATPQSRALRTVVCAEGYVAPPRGDEHTLGASFDFKSEDLAPTLAEHQGNLELLREISPDLLQRLGADDLPLERLEGRAAFRCTSPDYLPLVGPLAERAAFDEAYAVLARDARQVPERACPWLPGLYLNSGHGSRGLISAPLSGELLAAWICGEPLPLPRAVAEACHPNRFLLRDLVRGQRG