Protein
Type-2 restriction enzyme MboII
Function
Recognizes the double-stranded sequences GAAGA and TCTTC and cleaves respectively 13 bases after G-1 and 7 bases before T-1, leaving a single 3' protruding nucleotide.
Sequence
MKNYVSNINLGNSSLKFIDERLQSENYRGIHLSQHNRYDLPKLIDILTLLNKHAPNQSLMQIRTTDISKRPQNIPEEQSYAEFCNEAKSLTNIGTQDAMRKNLFVDFARMGLINRYNDKKVLTDPFKRGVTKYVALSDMGVKLIDPKLDILSKNLIFSKSLNKLLTGFVEDVLSLLTNSDLKEISFDEFMLFVSAMNCNFNFSISTEQCESLIKEYRLLSRVQKNAVIDTLKSELIPDNFNGDKKDKRDYHNWANENQQIWTLFENIPFFIMEKDSRKLILITSDVDLSKYSKSKMKRSQQAKNDYFKHHKVNKIKGYELDHIIPLLEAESVDEYRYLDNWLNLLYIDGKTHAIKSQSGSKYYIFTFDDNDYNQIYFLDTQGDKLSINNDDTALFDKNKVPKIYEYNQNFINAKTS