Protein
Endoribonuclease MazF9
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function
Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE9. Acts as an mRNA interferase, specifically cleaving between U and C in UAC sequences. May cleave its cognate antitoxin's gene (PubMed:25608501). In E.coli expression with non-cognate antitoxins VapB27 and VapB40 partially neutralizes the toxin.
Similarity
Belongs to the PemK/MazF family.
Sequence
MMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQAEQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS