Protein
N-acetyllactosaminide alpha-2,3-sialyltransferase
Organism
Neisseria meningitidis serogroup B (strain MC58)
Function
Catalyzes the transfer of sialic acid from the substrate CMP-N-acetylneuraminate to the terminal galactose residue of the lacto-N-neotetraose branch of surface lipooligosaccharide (LOS), forming an alpha-2,3-sialyl linkage. Thus, functions in the sialylation of LOS, which plays a role in the evasion of the host immune response by protecting N.meningitidis from complement-mediated serum killing and from phagocytic killing by neutrophils. In vitro, can use a number of different synthetic acceptors with lactose or galactose as the saccharide portion, but shows a strong preference for the N-acetyllactosamine containing acceptor.
Similarity
Belongs to the glycosyltransferase 52 family.
Sequence
MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKEKLFNEEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAERAYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEIKTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGLKNIMDDGRRKMTYLPLFDASELKTGDETGGTVRILLGSPDKEMKEISEKAAKNFKIQYVAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNVHVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKN