lhpD

Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2-carboxylate reductase

334 amino acids

Catalyzes the reduction of both Delta(1)-pyrroline-2-carboxylate (Pyr2C) and Delta(1)-piperideine-2-carboxylate (Pip2C) to L-proline and L-pipecolate, respectively, using NADPH as the electron donor. Cannot use NADH instead of NADPH. Is likely involved in a degradation pathway that converts trans-3-hydroxy-L-proline (t3LHyp) to L-proline, which would allow P.aeruginosa to grow on t3LHyp as a sole carbon source. Can also catalyze the reverse oxidation reactions, albeit at a much lower rate. Is also able to use Delta(1)-pyrroline-(4S)-hydroxy-2-carboxylate (Pyr4SH2C) and cis-4-hydroxy-L-proline (c4LHyp) as substrates, and might be involved in the metabolism of c4LHyp, a compound which is generated by the hydroxylation of free L-proline in bacteria.

Belongs to the LDH2/MDH2 oxidoreductase family.

35.684 kDa

MIRMTLDEVRELAVRILRRHAFSEAHVQAVADTLVAGERDECASHGIWRLLGCIATLKAGKVSADAEPELHDIAPGLLRVDAHGGFSQCAFRLGLPHLLEKARSQGIAAMAVNRCVHFSALWVEVEALTEAGLVALATTPSHAWVAPAGGRKPIFGTNPIAFGWPRPDGPPFVFDFATSAVARGEIQLHERAGKPIPLGWGVDEQGEPTTDASAALRGAMLTFGGHKGSALAAMVELLAGPLIGDLTSAESLAYDEGSRSSPYGGELLIAIDPRRMLGASAEEHLARAETLFEGIVEQGARLPSQRRFEARERSARDGVTIPEALHRELLALLE