Protein
Linear gramicidin synthase subunit D
Organism
Brevibacillus parabrevis
Function
Activates the 13th to the 16th (Trp, D-Leu, Trp and Gly) amino acids in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 14th (D-Leu) amino acid. It also catalyzes the NAD(P)H-dependent reduction of the C-terminal glycine residue of the N-formylated 16-mer peptide, that binds to the peptidyl carrier domain of the terminal module of this protein, to form a peptidyl-aldehyde intermediate that is released from the enzyme complex.
Similarity
Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence
MNNIETYYPVTPLQQGLIFHSLLEPESGAYIVQMGLKLQGPLNIPLFEQAWQCLVDRHAIFRTRFVGGKVKEYVQVVLKDLKISLVEHDLIHLSSSEQEAFLHHFAKEDRKRGFDIEQAPLMRLNVFHLNSETVHFLWTLHHVLIDGWSMPLVFGEVFAAYEMLSKGQPLSLPPVRAYRDYIVWLKKQDLQQAEAFWRTYMQGFTEATPLSFGRAYKNPYLDQKQYRELDLTVSEQTSKALQTLARQHRLTVNTIVQGAWALLLNRYSGQDDIVFGATVSGRPADLPGVETMIGLFINTLPVRVQVNAEESVINWLKTLQQQQADFRQYEYTPLVEIQGWSDVPRGQSLFESILVFENMPVGKSGGGESAISIVDVYSEEQTNYPFTLVAASGKTIDIKVKFDESQFELAAIERVVDQLHSLLSSIAKNAKQRIGDLSLISESERQQVLVEWNQTAEDYPSGLCIHQAFEQQAEKTPDAVAVAYKNRELTYAQLNERANQLAHRLIRKGVKPDTLVGICLERSPEMIIGILGVMKAGAAYVPIDPAHPQERIAYMVADSQASALLTQQSLLEILPVTAAHVICLDSDLLADEPVDNASSEVTEQNLAYVIYTSGSTGLPKGVMIEHHSAINLAYALIDAFDIQPTSRVLQFTSFSFDVSVSEVVMALLAGATLVIEDRESLLPGPELIQVLQEQRITTVSMVSSVLAALPDADLPDLHTLIVGGEAPSRELVARYAPGRQFFNCYGPTEATVCSTMMLCQAGMNNPPIGRPIANATVYVLDANLNPVPVGVPGELYIGGKGLARGYWNRPELTAESFIPHPFGTAGERLYRTGDLVRYRQDGNLEFLGRIDHQVKIRGYRIELGEIENAIRQHPAVQEAVVIAREEKAGDKRLAAYLVAAGKAQPPAEEIALFLKETLPEYMVPAGVVWLDAIPLTVNGKVDRRALPVPDWGQLSTKREYVAPRTPTEEMVANIWSQVLSVERVGSFDDFFELGGHSLLATQTVSRLKEAFGVDLPLRVLFECSTVNKLSEWIAAAGEDKSGLSRIPLVPVSRDRHLPLSFAQQRLWFFDRLMPNSALYNIPTAVRLQGELDMDALEQSLQTIIQRHESLRTTFTDHNGEAVSVIHPEIDWKLERIDLRERSEEMRNEAGLRLAKEEANRPFDLVTGPLMRATIIQTDERDFIFLLNVHHIIADGWSAGILIRELFHCYQAFAKAEAPQLAELPIQYADYAYWQREWLTSDVLDEQLSYWRAKLGGAEPLLALPTDRPRPAVQSYAGSSISLLFDDELRANLLALSKREGTTLFMTLLAAFQVFLYRYTGQDDILVGTPEAGRSRQETEGLIGFFINTLVMRTDLSGEPSFKEVLARVRETALGAYAHQDLPFEKLVDELNVERSLSYSPLFQVMFVLQNIPVQADALDGIRILPLEGSQQVETTKFDLTLTMAEAANGLAATFEYNTALFERNTVERMIGHFSSLLKAVAANANQAITALPLMSEVEEQQLVLEWNDTAVAYSTEQLVHELVAQVARDMPDQPAVVTRDQLLTYGQLEAKANQLAHYLQKQGVGRGSLVGICVERSVEMVIGQLAIMKAGAAYIPMDPAYPKERLAFMMHDASMAIVLTQAKLRQKLPADTSRLICLDADWETIAQEPTAALVNTTAASDLAYVIYTSGSTGTPKGVEIEHAALLNLIFWHQRAYDVTATDRASQIAGTAFDASVWEIWPYVTKGATLYLPEEEIRLVPEKLRDWLVASNITVSFLPTPLTESMLALEWPGDTALRYMLTGGDKLHHYPSEKIPFTLVNQYGPTENTVVATAGIVPKEAGQTAAPTIGRPIDNVQVYILDAHRQPVPVGVSGELYIGGSSLARGYLNRPDLTQERFVAHPFTEKAGARLYRTGDLVRSLPDGSIEFIGRADDQTSIRGFRVELGEVETAIVALPAVKEAVVTVCTDKQGTKRLAAYLVLEEGAALATGDIRKALKETLPDYMVPAFFTQLAYLPLTPNGKVDRKNLPAPDFQRPELEGEFVSPSTEKERRLAAIWKDVLGIEQIGIHDNFFELGGDSILSIQIVSRANQAGLSLAPKQLFEYQTIAELAEIVEEKAAVQAEQGAVTGELPLLPIQKWFFRLPLANRDHWNQSVLLSIQAGIDPAALKQAVGQLMFQHDAFRMRYTQSESGWLQAMDAPSETIPFRVEDLSQLAPEEQSSAIEAIANETQTQLSLRAGQVVQTIYFHLGKEVPGRLLIVAHHLVVDGVSWRIILEDLQHAYQQIAAGQEVKLPAKTTSYKEWAQELERYAHSEAFKHEKSYWLSKSSVHSTELPADMPDSAENTEATVKSVHFSLTVEETKALLQQVPQAYRTQINDVLLAALAKALGQWTGKRSVFVNVEGHGREELAEHLDLSRTVGWFTSMYPVHLQWDETFSVRRALLTTKEELRAIPNKGLGYGVLRYLHAEQEIVDAISRIQADVLFNYMGKIDQIVGSDSLFGSAPESSGANLCPSAQRHHLLDVNSVVAGEQLHVTWRYSEKLQRESTIAAVAESFMAALREIVAHCTLPEAGGYSPSDFPLAVLEQKQIDKHIGFDRQIEDVYTLSPLQQGMLFHSLYNQDSGDYVVQFAVTFQNLDVSVLEKAWQNVLDRHSILRTHFVWEGLSEPHQVVRKDVKVTLTKEDWRHLQADVQDEMLAAFLEEDRRRSFDIAQAPLSRWVVFQTKDEEYRFVWSFHHVLLDGWSVPIVLNELLAHYAAISEGREGKLVPSQPFSQYIAWLKRQDREKAKPFWTDQLKGFHEPTSLGMGKNVAASQQKQYKEQSVLLSEEATEHLQSFTREHQLTLNTLVQGAWGWILGSYSGEEEVLFGATGSGRPADLPGVETMVGSFINTLPVRVPLQTDATLLAWLKDLQRRQLEIREYEYTPLFDIQGWSELPRGSALFESILVFENYPTVQAAKKGEDEAASATSGVSLEIHDVAAVEQTNYPLTLVAAPGKQVAFKLKYDQDRFDDAMIERVLNQMTRLMVYMSKSPELRLNDVALMDEDERKQVLIDWNRTEKEYPRELCLHHAFEQQAAKTPENIALEYKEQSLSYAGLNERANQLAHLLIAQGVKPDTTVAICVERSMEMIIGILGVLKAGAAYVPIDPAHPEERIAYMLDDSQAVVVLTQAGLADKFTQAAAPVICLGEKLFADRAHVDVDNIQTDVASTNLAYVIYTSGTTGLPKGVAVEHRSAMNMVQAYIAYFGLDESSRVLQFTSFSFDVSVSEIWQALLSGGTLVIEDRESLLPGPDLVRTLRERRISKVSMASSLLASLPVAEYPDLAVLEVGGDACSRELVARYATGRKFFNCYGPTEATVGTVIKQLTLDDDTPTIGRPFPNTKLYVLDQNRKPVPVGVPGELYIGGECLARGYWNRPELTAERFVANPFGQPGERLYRTGDLVRYLPDGNVDYLGRFDDQVKIRGYRIELGEIAEALRQHAAIREAVVLAREVRPGDKRLAAYLTSAAEQELSVDEIKQWLKEKLPDYMVPASYTWLPAIPLNVNGKVDRKALPAPDWGQITAAYVAPRNPLEEMIANVFAEVLAVEKVGIDDNFFELGGHSLLATQTVSRLREIVGVELQLRTLFEHPTVAGLGEQLELLTKQSSRKLAPPIGKVSRKEPLPLSFTQQRLWFLEQFTQNSSINNIPSFLRIQGELDVAAWEASFSAIILRHESLRTSFEVRDGRPVQVIQPHGDWAMTRIDLRALEPAEREAEIKRLAEQAIVQPFDLTKGLLLRASLVQLDANDFVFLFVMHHIASDGWSMGILLSELMTNYKAFRQGEASPLGELPIQYADFAVWQREWLSGEVLAEQLGYWREKLKGSEPLLQLPTDRPRPPVQTYEGEKMSVQFGAELLKQLQSLSRKEGATLFMTLFAAFQTLLYRYTNQDDILVGTPIAGRNKQETEQLIGYFINTLVLRTDMSGHPSFRELLARVRETALEAYAHQDVPFEKLLDELQLERSMSYSPLFQVMFILQNIPVQAEPAGDIQLSSFDLELGAVTSKFDMTVTMVETPDGLLATLEYNKALFDSSTITRMVEHFHKLMEEIVANPDQSITLLPLMREEEEQLLITEWNRTEVPYSREKCVHEMIEEMVSKAPDSIALIVGEQRVTYGELNRQANQLAHYLRKQGVGPEVLVGICAERTVEMMIGLLAILKAGGAYVPIDPAYPAERIAYIIGHSQIPVLLTQEHLLPTLPEHQAKVICLDRDWATVAVESEENPGKLATSDNLIYVIYTSGSTGNPKGVALEHRSVIYFLSWAHDTYTPEEMSGVLFSTSICFDLSVYEMFATLTMGGKVIMAENALQLPALPAADQVTLVNTVPSAATELVRMKGIPASVRVINLCGEPLSNRLAQELYAFPHVEKVFNLYGPTEDTVYSTHAIVTKGATNEPLIGRPQFNTHVFVLDSHRKPVPVGVPGELYLSGSGLARGYLHRPDLTAERFVQNPFREPGARMYRTGDLVRYLPDGNLQFVGRVDYQVKIRGYRIELGEIESVLNRFPGVKEVVLLAREDREGDKCLVAYIVFEADCTSKIHDLNHFLADKLPAYMIPQHYMILDSLPKTPNGKLDRKALPKPEYDRSEAGVEYVAPQTPVEIMLHAHWAAVLEMETIGVHDNFFEIGGHSLLATQLIFKVREELQLEVPLRILFETPTIAGMAKTIEEIIKHGLTSVSQEIDAKGLQDEVALDPAILAEQPYEGDPSQFQAALLTGATGFLGAFLLRDLLQMTDADIYCLVRASGEEEGLARLRKTLQLYELWDEAQAHRIIPVIGDLAQPRLGLSAGQFDALAATVDVIYHNGALVNFVYPYAALKKANVIGTEEIIRLAAAKKTKPVHFVSTIFTFASEEGEESVAVREEDMPENSRILTSGYTQSKWVAEHIVNLARQRGIPTAIYRCGRMTGDSETGACQKDDLMWRIAAGIIDLGKAPDMSGDLDMMPVDFASKGIVHLSMTEHSVNSNFHLLNPNATDYDDLIAAIENKGFELERVTMDEWIEAVQEDAKDKGMDANSAAPLGNLFSDGHSSRGSVVYVGNKTTRLLRQADIECPEIDEEVFAKVLDYFARTGQLRVTQNTRN