Protein
Linear gramicidin synthase subunit B
Organism
Brevibacillus parabrevis
Function
Activates the 3rd to 6th amino acids (Ala, D-Leu, Ala and D-Val) in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 4th (D-Leu) and the 6th (D-Val) amino acids.
Similarity
Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence
MSRKKVDNIYPLTPMQEGMLFHSLLDEGSESYFEQMRFTIKGLIDPAILEQSLNALIERHDILRTVFLLEKVQKPRQIVLRERKTKVQVLDITHLSEGEQAAYLEDFAQKDRQASFDLAKDVLIRLTLVRTSADTHTLFWSHHHILLDGWCIPIVLNDFFQIYQQRKGGLPVELGPVYPYSTYISWLGEQDAEEAKASWAEYISGYEPTSFIHKQGGKNSYRQAELVFAIEQGLTDSLNKLAKQLHVTLNNLFRAIWGLMLQRQCNTEDVVFGSVVSGRPSHLPNVEQMVGLFINTVPIRVQAGAEQTFSELVKQVQQEALSLAKYHYLSLADIQGNQQLIDHILLFQNYPMGQQFLTRLNQYNEEFTLTHLSAFEQTNYDLNVMVTPSDVITIKYIYNAAVFSEEQLLHISRQLTTIMTQVTNAPDILLQKLEVVDPAEKQLQLHSFNDTYRHYPTDKLIHQIFEERAEREPERIALVMGEQVLTYRELNEKANQLAKLLRARGIGPESMVSLLTERSAEMMIAILAIFKAGGAYLPIDPSHPKERIEYILQDSRSELLLVNHRFLGAVDFADRIIDLEAAEIYQGAADNLECVSHANHLAYVIYTSGSTGKPKGVMIEHASLLNIIFALQELYPLLENDAYLLKTTYTFDVSVAEIFGWILGSGRLVILDPGAEKEPAHIWETMVNHGVTHVNFVPSMLIPFVDYVRDQQQESPLRYIFAAGEAMPSELVGKVYEALPGVILENIYGPTESTIYATKYSLAKDSQDVLVPIGKPLANIQTHIVNKHGQLQPVGVPGELCIAGASLARGYWNNEALTNEKFVPHPFAAGQRMYRTGDLARYRQDGNIEYLGRIDHQVKIRGYRIELDEIRAQLIQEASIRDAVVIARTDHNGQAYLCAYFIADKQWTVNALREALRQTLPDYMVPSHFIQMEEFPLTSSGKIDRKALPLPDGRVHTGNVYLAPRNPVEELVVRIWEEVLNVSQVGVHDNFFELGGHSLLATQVLSRTAKLFHVRLPMREIFTHQTVAELARRIQALRHGAEADKHSPIQPSALQRADELPLSYAQQRLWFLDRLIPDSAMYNIPVGFRLRGTVDELVLERALNEIIQRHESLRTTFVDVDGRALQVIHTDVHLSLGVTDLRDKPAAAKDAEWKQMAEEDAATPFRLDQWPLLRAMLIRLEEQESVLWLNVHHIISDGWSMDVLVNELSEVYETLLKGEALPLAALPIQYRDYAVWQREKSQDDVWKEQLRYWKNKLDGSEPLLPLPTDRPRAVVQSYRGDHLSFYVPGEVGQKLRELGRQEGATLFMTLLAAFKSFLYRYTHANDILIGTPVAGRNRQEIENLIGFFVNMLVLRTDLSDDPTFVELLRRVRETAFDAFANEDVPFEKLVDELQIERSLSYSPLFQVLFAVQGMSTGVREGETLAIAPDEVTLNQTTKFDLTLTMIEAADNGLKGVFEYSTDLFDRTTIERMAEHFGNLLQAIAADPGQKIVELPLLGGAEQSRMLVEWNQTDVAYSLDLLVHERVARIAQELPEQFAVIGEQGALTYAQLDAKANQLAHALLKRGIGSEDLVGICVERSSEMQIGQLAILKAGAAYVPMDPAYPRERLAFMIKDAGMSLVLTQERLLDALPQEAAALLCLDRDWQEIAAESTAAPAIKTNADQLAYVIYTSGSTGTPKGVEIEHGSLLNLVNWHQRAYSVSAEDRASQIAGTAFDASVWETWPYLTAGATICQPREEIRLSPEKLRDWLVETGITISFLPTPLAENLLPLPWPTGAALRYMLTGGDTLHQYPTADVPFTLVNQYGPTENTVVATAGAVPVLGERESAPTIGRPIDNVSVYVLDENRQPVPVGVVGELYIGGKSLARGYRNRPDLTEASFVPNPFSPIEGARMYRTGDLVRYAADGSIEFIGRADDQVSIRGFRVELGEIESALYAHPAVAESVVIVREDVTPGVKRLVAYAVLHEGEERQTSELRQSLKEMLPDYMVPSAIVLMEALPLTPNGKVDRRALPLPDVAQTEWEGSFVEPQSDVERKLAEIWQEVLGVETIGVHDNFFELGGDSILTIQIVSRANQAGLQLTPKHLFDAQTLAELAASAVVLEKAPEMQAEQGIVTGELPLTPIQTWFFEQDVRHVHHWNQSVMLAVREELDMTALTQAFAALPRQHDALRLRFQQVNGTWQAAHGEIADEDVLLVADLSSVPEAEREARMRHITDELQASLDIEKGPLHRAAYFQLGAEQRLFIVIHHLVVDGVSWRIILEDLQTAYEQVKAGQKIAWPQKTTSFKSWAEELTTYAEQSAVDEYWTGMDSEQACGLPVDHPQGKNTEGLAVQVKAKLSADETRALLQEVPAAYRTQINDVLLSALTRTITDWTNKRALYVSVEGHGREPIVDGVDVSRTVGWFTSLYPVLLETEPDLAWGDLLKSIKEQVRAIPDKGIGYGIHRYLSRDGQTAEMLRAKPQPEISFNYLGQFGQGQTTDAALFQIIPNWSASNVSEDETRLYKLDVMSMVAQDQLEMSWTFSRDLYEPGTIEKLAHDYVQALRAIIAHCRTEQAGGYTPSDFPLAELDQNSLDKFIGHNRLIENVYTLTPLQEGMLFHSLYEQAGGDYVVQLALKLEHVNVEAFSAAWQKVVERHAILRTSFLWSGLEKPHQVVHAKVKTFVERLDWRHLTAAEQEAGLQTYLEQDRKRGFDLARPPLMRWTLIRLDASTFQFVWSFHHMLLDGWSTPIVFQDWQAFYAAASHGKEASLPAIPPFSAYIAWLKRQNLEEAQQYWRDYLQGFGVPTPLGMGKSGGSAGQPKEYADHKLLLSERATANLLAFARKHQLTLNTVVQGAWALILARYAGEAEVVFGTTNLGRPTDLPDAEAMVGLFINTLPVRVLFPEQTTVIDWLQSLQQAQSEMRQYEFTPLVDIQSWSEVPRGQSLFDSIFVFENYLSGTSVDSESGMLLGEVKAVEQTSYPLTLVVAPGEELMLKLIYETGRFEQPAMDKVLAQLSSVLEAIMREPHEQLADLSIITEAERHKLLVEWNATDMPYERNLVMHQLFEAQVEATPDAQALVVGTERLTYAELNKRANQLAHYLRAQGVGPEVLVAVLMERTTEMIVALLGIIKAGGAYVPIDPAYPQDRIGYTLDDSQAAIVLTQERLLPMLPEHTAQVICLDRDWACMAVQPEANVPNLAAPTNLSYVIYTSGSTGLPKGVAIQHSSVIAFIFWAKTVFSAEEMSGVLASTSICFDLSVYEIFVTLSCGGKVILADNALHLPSLPAAKEVTLINTVPSAAKELVRMNAIPPSVRVVNLAGEPLPNTLAQSLYALGHVQKVFNLYGPSEDTTYSTYVQVTKGAKTEPTIGRPLANTQAYVLDAKLQPVPLGLPGELYLGGDGLARGYLKRPKMTAERFLPNPFHPDPDARMYSTGDLVRYLPDGQLEYLGRIDHQVKIRGYRIELGELEAVLRSHPQIKEAVVVAKEDKLGEKRLVAYITTKDGECGDRAVLTSWAKAKLPEFMVPSFFVWLDAMPLTPNGKIDRKQLPEPEWGQVASAAGYVAPRNQTEVLVASIWADVLGIEQVGVHDNFFELGGHSLLATRVASRLRETFAKEVPIRAIFERPTVAELSETLGAIGQNETEAQMLPVSREAHLPLSFAQQRLWFLDRLMPDSTLYNIPSAVRLLGDLDIAAWEKSLQVLIQRHESLRTTFGDVDGEAVQVIHSRLDGKLNVIDLRGMPADEREAEAHRLAGLEAATPFDLSQGPLLRTTLIRLAEQECVFLFNLHHIIFDGWSIGIFLKEMRALYEAFVREEAPELAEITVQYADYAVWQRKWLEGEVLAEQLAYWKEKLSGAEPLLALPTDQPRPAVQTHDGAMHTIKLSGELYAKLNKLSQEEGATLFMTLLAAFQVLLYRYSGQEDILVGSPVAGRNRQETEPLIGFFINTLVLRTDLSGEPTFRELLARVRETAFEAYAHQDLPFEKLVDELELERSLSYSPLFQVMFVLQNFQLNLDEKAGIRVADFEMDKHLVTSKYDLTLTMAEKQNGLFATFEYNTALFHEATMERLSQHFIQLLEAIVHMPDQGIARLPLLNQSERAQLLVEWNDTTTAYPRNKRVDQLFRETALLYPERLAVVAGNQTLTYAELERRANQTANYLQQKGVRPGALVGLCVKRSLEMLIGMLGILKAGGAYVPLDPDYPEERLAYMMGDAGITVLLTQEQLMPGLPSGERTTIALDRDWPLIAKESEQAPDVDTTAESLAYVIYTSGSTGLPKGTLVVHRGIVRLVKETDYVTITEQDVFLQASTVSFDAATFEIWGSLLNGAKLVLLPPELPSLAEIGQAIQSHHVTTLWLTAGLFTLMVDHHKEYLSGVRQLLVGGDIVSVPHVKKALEIAGLTVINGYGPTENTTFTCCNPVTVMPESAHTFPIGRPIKNTTAYVLDRHMQPVPIGVTGELYIGGDGLAEGYLNRPDLTAERFVPNPFATDQAARLYRTGDLVRYLPDGLIEFIGRLDNQVKIRGFRIELSEVEAVLAKHPAITASVVIVHENEAGMKQLVAYAVKDAEQELGTAELRQHFKAHVPDYMVPAAFVMLDALPLTPNGKVDRKALPAPVLERSREEDAFAAATSHVEQTLADIWCAVLRMDRIGIHDNFFELGGDSILSIQIVARANKAGIHLTPKQLFDQQTIAELAKVAGQSTKVDAEQGNVTGEVPLLPIQTWFFEQKQPTPHHWNQSMLLQVNEPLEEECLSQAVAQLLAHHDALRLRYTFADGQWKQTYADVDSEVPLQVEDLSMSPPAQQARKIEKLAQQAQASLDLQNGPLLKVVYFDLGYDRPGRLLMVIHHLAVDGVSWRILIEDLQTAYGQAEKGNKIQLPPKTTSYKAWAEKLHKYASSERMLVDQDYWLKAADELSGHPLPVHDWAENTEANGRMWTIHLEEEETDALLQKVPSRYRVQINDILLTALALAYGKWTGESALLVNLEGHGREELFEDVDLSRTVGWFTSMYPLLIQLEPNTSSEDALARVKEKLQQIPHKGLGYGLLRYMAQDPELVEKLKAIPQAPLSFNYLGQFHQAADAKALLAYAEGERGANSGPDNRRTHLIDVVGAVTEGKLGLSFLYNGRLYSESHIETFARHYTDALQSLIQAEKQSYRAEDFEDADLSQSALNKVLARLKNRKGNELHGGSH