Protein
Isoleucine--tRNA ligase 1
Organism
Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Function
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).
Similarity
Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.
Sequence
MEYKNTLLMPKTEFPMRGNLPKREPAMQEKWAEMNIYEKVQEHTKGRPLFVLHDGPPYANGDIHMGHALNKVLKDFIVRYKSMTGFSAPYVPGWDTHGLPIEQALTNKGVKRKEMTVAEFRKLCAEYAYEQVERQREQFKRLGVRADWDNPYITLEPAYEAQQIKVFGDMAKKGYIYKGQKPVYWSPTSESALAEAEIEYQDKKSASIYVAFPVKDGKNVLEGDEKYIIWTTTPWTLPANLGISVHPELEYSIVKVNDEKYIIASELFETVAKTLEWENAEVVKTVKGSELEYTVAKHPFYDRDSLVMLGDHVTTDAGTGCVHTAPGHGEDDFVVGKKYGLEVLCPVDDKGVLTNEAPGFEGLFYDKANKPITEKLEEVGALLKLTFITHSYPHDWRTKKPIIFRATAQWFASIEAFRKELIEAVAETKWVPAWGETRLHNMVRDRGDWCISRQRAWGVPIPVFYAENGDPIITDETINHVADLFREHGSNVWFEREAKDLLPEGFTHPGSPNGEFRKETDIMDVWFDSGSSHQAVLEEREDLQRPADLYLEGSDQYRGWFNSSLSTAVAVTGKAPYKGVLSHGFVLDGEGRKMSKSIGNIVVPKKIMDQLGGDILRLWVSSVDYQSDVRISDDILKQVAEVYRKIRNTFRFLLGNLDDFKPSENAVAVAELREVDRYMLVKLNDLITKVKEAYETYDFAAVYHAIHNFCTIDLSSFYLDFAKDILYIEGANHEDRRAIQTVLYDVLVALTKLVTPILPHTADEVWPYIPGVTEESVQLTDMPEAVQLDDAEALKTKWDAFMTLRDDVLKALEVARNEKVIGKSLNASITLYPTAEMKAMLESISEDLKQLFIVSEYKLGGMMDEAPADAPKYEHTAVVVAQATGETCERCWVVSETIGKDAEHETLCERCATVVKENYVK