Protein
Isoleucine--tRNA ligase
Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Function
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).
Similarity
Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.
Sequence
MTKRTFAPVPTQPNFRQLEADILQKWKDEQVFEQTQTRPAPAGEFVFYEGPPTANGKPALHHVLARSFKDLFPRFKVMQGHHVTRKGGWDTHGLPVEISVEKKLGWLGRNHGASREELEEFNRLCRTSVWETIQDWNELTERMGYWLDLGDPYITYQNSYVESVWNLLRRLHEKGLVAQDYKVVPLSPRISSTLSRAELGEVDSYRMVDDPSVYVRLPIVWDTLPERAHAALSSLSGEQRQGLSLLVWTTTPWTLPSNTLAAVNPDLDYVVADSPSGRVIVAEGAVERLSALHKDAAPLEVLARFKGRDLEWVEYEPPFPEVASQLGVVSELHERRDGKPVLHFVVMADFVSDVDGSGVAHEAPAYGAEDLEVARAYGVPLMFGVDDHGILQVTHEQGKFFKDADKGLIADMKARGLMFWAGTLKHRYPFHDRTGDPILYFAKKGWYIRTSQVAGEMLAQNEKINWVPGNIKHGRFGNWLEGNVDWAISRERYWGTPLPFWQSESGQLRVIGSVAELSELAGRDLSDLDLHRPYIDDITFTLDGEEYRRVPEVLDVWFDSGSMPYAQWGLLLNEQGEAVRGAEQFAKHYPADYICEAIDQTRGWFYSLHAISTMLYDQPAYKNVICLGHIVDEKGLKMSKSKGNVVAPLPLFDQYGADSVRWYMFMASDPGDQKRFSERLVAEAQRSYVNTLWNVYSFFVLYANLDQPDLAAAPAVAERPEMDRWLLARLEETVRDVTAALESYDARSGGRALEGFVDDLSNWYVRRSRSRFWGEGGTVDTAAYATLHEALLVVSQLTAPFTPFLADALYRNLSGEESSVHLTPWPTVRAERLDRKLTADMAAVMKVVELGRAVRGAHNLKTRQPLAGVQVRAASPEALDALKRSQTQIMEELNVKAVTFLEGDTDLVQYSLRPNLPVVGKQYGKQLPVLKKALTEADARAVATAVQAGQGFSVQADGVTFDLTPGSVLVDAKAPEGVAAAEDAGYLVAFDTALTPELVREGLARDLVRAIQEARKAAGFEVQDRIALALELDGEALEAAQAWQDFIAGEVLAEQVAYGSGEGFRAEVEGGAVTLKKL