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hiw

Gene
hiw
Protein
E3 ubiquitin-protein ligase highwire
Organism
Drosophila melanogaster
Length
5233 amino acids
Function
Atypical E3 ubiquitin-protein ligase which specifically mediates ubiquitination of threonine and serine residues on target proteins, instead of ubiquitinating lysine residues (By similarity). Shows esterification activity towards both threonine and serine, with a preference for threonine, and acts via two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates (By similarity). Required in the presynaptic motoneuron to down-regulate the levels of wnd and restrain synaptic terminal growth at the neuromuscular junction (NMJ) together with Rae1 and Fsn (PubMed:10839352, PubMed:21874015, PubMed:17697379).
Similarity
Belongs to the RING-Cys relay (RCR) family.
Mass
565.631 kDa
Sequence
MVGLLNPLKYGDHFYELYTNSTRRKMQHDRKVLTKRKSRKDKSAAAMAAAAAAAVLEGNAGAGGLVNPLDPPLVKEQLMILPSFPVAHIELDPNASKFAVFSAIRSTVLEAETRYSLFQEGGAPSGQSGSNQKKWSNNVNSACCMMCAHSCMISNLLDCSCHAHMAMHGAVGPGNAGPGGPVGVLVDGRIPAPVPVVVGGPGLPAEKRPRRDSANSDADSDTEEPTEREPVYATVPLIVGAGLRSLFELIADARHVHPLLCTKALKALLDVIQGQQPESFKLEPEELINPLYDLLLDLATMPAALNSATGAEANWSAMACAALLGLCIARGDTGKMLKAIAAMLMTPRQLSAQIVQLPVVLATLQHTVISAALNKPTRPDFHSHGVPHNSLIDEFPVKLPPLSTGAPITSPAMACDGVFVYLLYGGTLLKIGTGFGGSYKGHIYAQNEDFSHERSAWLGYSGGQLYFRRTCRRSAGDQLQMVGLDTLAIKAMSPLSMLHMREGLNYVLFTDDDSLHAICSNRDDTLVVKKLNLYHNSYNIDPPPFELPLQLARKKFRTLGYAAFEDELLNQYQIQRIQSAHNSFEPKLPAPCRDADVDVMGMACGKEFGLVRASNGRVYYYGKSAALGLKCVGRTPTLKLTELVISKAANIVHVAVGHDGIHALLVNDDGTVFFAGTARRGEDGDSSKNRRQPKAVKPKKMTKIDGHVVVHAACNNGTSAFVTKTGKLIMYGKDTAHCDAMGFVSELLEQHVTKVALGKAHCVALNAKGQLFSFGLNNKGQCGRIFNKLQPVKDVPPFASSSTAAACFASLLPLDKRLKLDFSTLCDYDDHNLVQGQCRVCVICRECTGYNVSCVSALNVPLDQRLAGSICPCGHGDAGCAKCGLCAACIALQDSDEAKTELKPPPSDVQQRQQRSKTLIMRRKERKGELETGAAGGGAATPTDLDKDPPRVAPLAPQLLQLTSSSPVVQVACGLHHTVVLTLAGEVYTFGSNQYGQLGSGDLQPVSGPVRVQVPGAISQVAAGSNHTVLLTSKGMVYTFGNYQKGQLGRLPSDYGLKPPPQDDDSPVGAGSDGGAGGSPTVAPVGMPGPERSQSPANVQPSGSKEMPPLLPVLTQRQKFLWNCSPGAVFGLGPCYGKKVTWIGANGDQTFIKIDESLITAQMLPKMHVVANKKTILLIPSIPLSFHTLSINRRDGSCTAHYRGQTNFVKLMQAQPEQQQPEINSNLDVAVTPVADSPAHASTSSLLAALTGTATAGVPINEQMSRSMHEARNQIFEEQLPEVGSSAAAAAVAAPGTPVSAGSVPRSRRGGKQGTSSPEPIPSPPQLAFTMDPTYNVLWVFDGAARKLRCHNVVASDINDSDANAATYRSLLSPELSLPDRVDSRVARSQASLNLLACLDILTSAQDNIPGCFEQPLLKQTQQTAETQAGEFQVVNRFDNFGGGWGYSGHSVEAIRFSADTDIVICGFGMFGGRGEYSCKLKLFDLGGDGGGYEKEGILISETKEVPYECGARSKHHILLPKPVSAVAGRWYLVWARIAGPSSDCGSCGQASVTTEDQVVFSFKSSKKANNGTDVNSGQIPAILYRLVTQDCKQTPAQMDADPVQRISRAFANSVSRECFESLVVLLSWSWDCFKLQLREERDRSRPLQLQQSLQYLGYVIKSCLRLLRKYTIEIYPQRNSSTSVATGGGSNAAHGSGVVTTAKSVQSKPNKDKNTPRVVGNAGVMAKYFGDPSTSVAPAMISSASSGGAPSTSASAAVAPGSGTPVTRKTNMENIQLAECIGNVRALLIGIFCDDIFKDIATDEGYELSLEILDECHLSFVACFDAFYPTSSLKWNCLCDLLAQMDRGALHSRLLSAILAGLCSPSVKLRATFSLLSAAGNERQSIISPSDNSGLPMLSSTDAHPYPVLVEQMIYRTQQEKSDFLSNSWTFKDVLVRLLDIIASPIRSRIEAIYSRSLGSLGYPGGKDCVNQGLIDNCCHLLARVLAEIVYQTAMGEYDKLFMPPRTLHSTGARFARCDVSRTWNTGNFGPDAIAFAVDRPGVAIAGAMVYSGSGSYDYQLELLYDNTADLQPQHKWETLESVSGSYDQDAVHNDLAEIKFDHPVHIKENARYALRLCSQGARTCSGDAGMPAVRGPCGAQFHFYACDLSFNGTTPARGQLPCILYYSTPMKQDGHSASGRTGDGSNVATHLEDRIMLLGPHEVSTRDTALQIAADITKKCTELLILARNAMAASCSPSDNSSNHTQTIDSEHNITPIEEHMDINWANNSRTAALPTAIDPQLSTARDLGKRIESFSKGLMETLKFDKRSTNPFEMEIEIGATEVEESADLRNGQSQSVSQSQSQSQSVPINGNERTADFEFAEQSAQQSMPQHLHSDSEEAPLEVAGMAAGGGVSVADGSGGVAGVGSQAAAVQLLEVFNLAASNMFHTLLPLVYAHIANLACSDPKSSVQILGLIKEILPHIAALNQLHVSKDQRQPEPAIFATQTSGSGNSNSSSTTSNHYCVVESDHPYKSASISSYRVEFPPCVQWLTIEFDPQCGTAQLEDYLLLSIPMRPASQAPPVPHVDDYLEQADNNVNGAGDRRRNTGGGIAGSGAAPNTHQRSASVQLTMASCCRSPGCGNAPGSAAAPSSMPLRSQDPNDREWIVVKKFNTASTWLHNVLILPGNCVEFSLETASLYAQDPHNNRYGFKCLVVGYDNPTSINASNSCLIRLEQELAYLGGMCSANLMKKELNLPDDKDVEDMSGIEETINTHHTLLSKGFALSEPQLTVHQALESYLPIGSQSNERQFLKDFISGAPGSSGARLAAWLQPESRLDPNKCELNTITEPLRYGWPSQVTVTIRDQYGDAVLVPELKVEIKAIPTGSGPNGSATGTGTSCTSVAEVSAPGPNLWMRRASRDTWGWGGMAPPPRINYEPTVKDKMVFKAITFMKPYANYSFEELRYASPVQTRVTELLNAKDMEDGTFSVQWTPSSVGAYCLAVTIDGIPLEEVYRVDVKEGILPPPTQRNSAQRRPQAPSKLRRFQARHSSGLRIRSHPTLQSEQVGVVRVGGVISFIDEIENDDGVWLRLSTESIRQHCTMGWYPTEAWCLQFNQHLARMLLQPVTDKEVNPVRKGVGAEEDVEEQPPVTPSASGEASPEPEPDPSPVLSPAKTKPGRFLSGHQSTNPFLYPAKHADLAEREAQVQEEREKEEEQVDDEDADDREPEQEALPAVELLPAHIGSAIAGVVGGGAIKLQALQKWFKGDAVDGPQPLTPSHSPPLAGVSVRELVRAMGGQDSPRGNGNRSQQEQDPEFSLASMRRPNYSASQTAALLSTPKHTPKRSAVVASETSGLEDELSLLQITTTTTGQGEQQSELQLATTSTASSASKRNPMGPIKRAMPPSFAESIRAVFAALLWHEGVVHDAMACASFLKFHPGLPKEGATVVTRRGESGDPRLQLSREQKAQQRHSVEVANAGNYLNIRPSTLETLTKSGNCSLHNRSKYRKNLLSGGGGAINSGDDTAQKLQALPEMVSVLPPALRCLVYLWEQICSGCVQIVQSNALEQREPRLLSPGSRDLNGDADTEGKEGKNSDQASAGEKDLGRKCKRKKKDDGSWCEICELFLPMPVTYHMRIAHPGCGKSAKGKGYNSVGIFCEGWAGNCGEGGKGASSWFLMCDPCRDRYLASCRSANNINSAARQLESSAAEGNELNLFGVKSTTLIANAEVYTTMRENATFLLELCSSSSSASGAAGSLAATSSSSKRSPQQMSVVAMPVVIEHQLGNSDLKPSTSRCSRMARLSGSKFCPGVGSGAFRKSFVGGPPTAPENVWLAPESFACLECLGTAGHEDLPYEMFGLGPNSNDNGYDRPLSEISYESCEPNNYDMLSGSLAPGTTAAASVGGGNLSKFHRSYSMGQGWASLAQHNHPPPHHPQQQHHQQQQMNLQLQQHQAPPVDGQPKVVYRRRNNSTSEGDGSLLICYPSEHLRRLVPQKLLASVSVMQTASGEGTGKDHATGTLGLDQSAGQNGGGNLLLTRPAMAFITQKHELDRLRAAMRRSLRIAACRIYALQALNWLLRSVTQGVCLHDLMWWFVSSLNPTGGHQPVERGEEASEPALEHPVAYTQISGRFAHLITQSLHVFLQSVADLTLHLPLGSPLQRVAIQCFGIRFRQADHQFLHSSHVFGNISKILSKSDEQNDAMAVSTILKPDCDVEHNQVHSVATGGSSGAGARLLCYTDLAGMFEVTVSSRPAMAESLTDNSTETFWESDEEDRNKCKIIELSLTKLNYACRYLLVHIDNSRDIQNKVLNVVFYAGQSLGDTNIIKSADVDPKACSWISAKICDDSCTHFRLELHGPENTLRVRQIKLLGLPIGGAVGSDDSSDHKHQPHLRLSHASRIQQQICEAETLRVFRLITGQVFGKLISNVSSDLVPPDSAGIGPPSGGAASTSLLADSLDLREHMVGILFSRSKLSHLQKQVIVHIVHAIRKEAQRAKEDWELANLAHVLKQSPQQQTAPALAASASCESTPERSRAPDTYCFEMLSMVLALSGSVVGRSYLSQQHGLLRDLLGLLHTGSDRVQRQVTALLRRILPEITPESFAELLGVQRLPPADYSIAHQSASDFDMSRLGLLDIFLAVIAKSLQLQVKVKTTVASTGPSGSGGVSGSSSGNGGAVLKAGQQEKTPAFVRLWSSLDLSVQQLRSRPPTGEPGTTDPFQFDALPPRKESKRNLNQRWFLNGVISTKQAESIISLIRDLASGKLSEKWSQITKAAIAESVLNLTRLEEIYRSPEHCTKTSTLWLALASLCVLERDHVEKLSSGQWSKLCDTRPLCSNHDDGETAAIIQCETCGSLCGDCDRFLHLNRKTRSHKRTVCKEEEEAIRVELHESCGRTKLFWLLALADSKTLKAMVEFRDGSHTIISGPQEAVGRCRFCGLTGNSGLLEIGNVCADAQCQEYAANSCLKTKPCGHACGGVTGERKCLPCLQHVCHTRENELAEELRDPKLTQDADDMCMICFVEALSCAPSIHLECGHVFHYHCCKAVLEKRWSGPRITFGFSLCPICKADIQHPLLSDILEPINGLKQDVKRKALMRIKYEGVVKDTDSKNVNMTQLAMDRYAYYVCFKCQKAYYGGEARCDAEIGEKFDPEELVCGGCSDVARAQMCPKHGTDFLEYKCRYCCSVAVFFCFGTTHFCDTCHDDFQRLTNIPKVKLPQCPAGPKAKQLLGDECPLHVMHPPTGEEFALGCGVCRNAQTF