Protein
Bifunctional F420 biosynthesis protein FbiB
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Function
Bifunctional enzyme that catalyzes the GTP-dependent successive addition of two or more gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives, and the FMNH2-dependent reduction of dehydro-F420-0 to form F420-0. Is able to add up to six glutamates to F420-0, producing F420-3 as the major product with lesser amount of F420-4,5,6, consistent with it producing the polyglutamated F420 compounds present in Mycobacterium smegmatis.
Similarity
In the N-terminal section; belongs to the CofE family.
Sequence
MSAAANAEHGAADRVEILPVPGLPEFRPGDDLVGSLAEAAPWLRDGDVLVVTSKVVSKCEGRIVAAPSDPEERDTLRRKLIDDEAVRVLARKGRTLITENAIGLVQAAAGVDGSNVGSTELALLPVDPDRSAATLREGLRERLGVTVGVVITDTMGRAWRTGQTDFAIGASGLTVLQGYAGSRDRHGNELVVTEVAVADEIAAAADLVKGKLTAIPVAVVRGLRLPDDGSTAHRLVRAGEDDLFWLGTAEAIELGRRQAQLLRRSVRRFSAEPVPHDAIEAAVGEALTAPAPHHTRPVRFVWVQDSETRTRLLDRMKEQWRADLTADGLDADAVDRRVARGQILYDAPELVIPFLVPDGAHSYPDDARTAAEHTMFTVAVGAAVQGLLVALAVRDIGSCWIGSTIFAADLVRAELELPDDWEPLGAIAIGYPEQTPQPLGPRDPVPTDELLVRK