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eqx3

Gene
eqx3
Protein
Diels-Alderase fsa2
Organism
Fusarium heterosporum
Length
377 amino acids
Function
Diels-Alderase; part of the gene cluster that mediates the biosynthesis of equisetin, a trans-fused decalin-containing tetramic acid with antimicrobial activity (PubMed:23614392). The PKS module of eqxS together with the enoylreductase eqxC catalyze the formation of the polyketide unit which is then conjugated to L-serine by the condensation domain of the eqxS NRPS module (PubMed:23614392). Activity of the Dieckmann cyclase domain (RED) results in release of the intermediate N-desmethylequisetin also called trichosetin (PubMed:23614392). Diels-Alderase eqx3 is involved in endo-selective Diels-Alder cycloaddition to form the decalin ring of equisetin (By similarity). Subsequent N-methylation is carried out by eqxD to give equisetin (PubMed:23614392).
Similarity
Belongs to the Diels-Alderase family.
Mass
41.224 kDa
Sequence
MSNVTVSVFTLDKSISEEPVLPSSFIPSSGNVFPKFTSAIPKTAWELWYFDGISKDDRSSIVIGVTRNAEGLKHGGFKVQVFVIWADERTWHRDLFFPESVVSIDESGATEGIWKHATSSSSISFSCAGDLSKASLVFDVPGIVQGDMHLEALPGDTGLDTDATLGPSVYYVRPLGRASVKAQLSLYSSDATAAEQFILGTSANGGMDRVWSPLSWPQVMTESYYLRAQVGPYAMQIMRIFPPKGSENSENQPSTMARLYREGQLICAPQHVVTRDDALITHDSLILSKQNTSDSGDAVTGEYRDKNTGYTVEFVGKGNEEQRWEFQVRHERIIWNTPTSRPGPDATGNTGFVERLYGGTIGESYEGVGTGGQCELS