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endA

Gene
endA
Protein
Endoglucanase A
Organism
Fibrobacter succinogenes
Length
453 amino acids
Function
High levels of endoglucanase activity detected on acid-swollen cellulose, ball-milled cellulose, and carboxymethyl cellulose; moderate levels detected on filter paper, phosphoric acid-swollen cellulose, lichenan, and xylan.
Similarity
Belongs to the glycosyl hydrolase 9 (cellulase E) family.
Mass
50.042 kDa
Sequence
MNCRKYLLSGLAVFGLAATSAVAALSTDDYVEAAWMTTRFFGAQRSGQGPNWILDGTSNPTSFTKDSYNGKDVSGGWFDCGDHVMYGQSQGYASYVLALAYAEFTEVSTTFILVTTPTTRKPTTTPMKSGKPNKVRDLLEELRYEADFWVKAAIDGNNFVTVKGDGNADHQKWVTAGAMSKLGSGEGGEPRCITGNANDGFTSGLAAAMLAVMARVDPDTANQAKYLKAAKTAYSYAKSHKGVTNSQGFYESSWWDGRWEDGPFLAELELYRTTGENSYKTAAIDRYDNLKFSLGEGTHFMYSNVVPLSAVMAEAVFEETPHGMRKEAIGVLDLIYEEKAKDKIFQNPNGMGSGKFPVRVPSGGAFLYALSDKFNNTNEHMEMIEKNVSYLLGDNGSKKSYVVGFSKNGANAPSRPHHRGYYANEKRWRRSRRCSESSRKEQALGRYDCWRLY

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)
Length
353 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
40.191 kDa
Sequence
MNLLKTQLKGDRVLAGKEAVAELYKTGYFGRPKDDGLELSLVEAAYLQSRGKLDIELEGKLLGFRAFFEQASLRQQNFELKYIVYKDLKERGYYVQPSAADFRVYPRGSHPGKSAAKIFVHVLSERQPLSVKLLQESVASAENVHKQFILAVVDEESDLTFYEIKSASPKGEMPEPFPAVKADATFLEDRVIAWDAEASGALYSRGFYGKMLDPERLQLSLVESLYLFSRGVIVVRDRKGKIFSFDEFVEKASEIEGSFLRKYSAYKALRDSGHVVKTGFKFGTHFRVYRKVESIEKIPHSEYLVNVIPEDYEFRLPVMSGAVRLANSVRKRMLFAVEKGEEVEYLDIGRVKM

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Length
351 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
40.465 kDa
Sequence
MRAEIVKDRVLVEKKAINEFYNNGYYGRPKSSGLELTLIEAVYLAFRGKIEVEHEGKVLEFSDLFKEASILQPSFELKYIVYKDLRERGFYVQPGVTDFRVYPRGSHPGKGAAKQFIYVRSERAPMPLRDLLRSLAAAENVRKQMVLAIVDEESDITFYDVKRPRLKGEMKEPLYPDINADATFLEDRVVVWDEEASKTLFENGFYGKPLDSQRLQLSLVESRYLLEKGVLNINNRQDESMDVDAFSKMASEIEPEFNLKSSVYTDLRDKGVVPKTGFKFGSHFRVYSQVESPTKIPHSEYLIHSIPMDHEFTLPVMSRAIRLANSVRKRMLYAILTDDGVDYIDIGRLKM

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Length
350 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
40.133 kDa
Sequence
MKTQLEGDRVLAGKEAVAELYKTGYFGRPREDGLELSLVEAAYLQFRGKIEIELEGRKLDFRALFEQASLRQPNFELKYIVYKDLKERGYYVQPSAADFRVYPRGSHPGKSAAKIFVHVLSERQPLPVKLLQDSVISAENVHKQFILAVVDEESDLTFYEIKTASPQGEMPEPYPEVKTDATFLEDRVIAWDAEASGALYAGGFYGKMLDPERLQLSLVESLYLFSRGIIVVRDRKDRIFSFDEFVEKASEIESSFLRKYGAYKALRDSGHVVKTGFKFGTHFRVYRKVESIEKIPHSEYLVNVIPSDYEFRLPVMSGAVRLANSVRKRMLFAVEKEEGVEYLDISRVKM

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Length
350 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
40.401 kDa
Sequence
MKTQLKGDRVLAEKEAVAEFYKTGYFGRPKGEGLELSLVEAAFLLSRGKLEIELEGTMLDFRYFFEQASLRQPNFELKYIVYKDLKERGYYVQPSAADFRVYPRGSHPGKSAARIFVHVQSERQLLPVKLLQDSVISAENVHKQFILAVVDEESDLTFYEVKTAVPKGEMTEPYPAIKTDATFLEDRVIAWDAQASETLYKRGFYGKMLDSERLQLSLVESLYLFSRGIITVRDRKGKVFSFDEFIEKASEIESFFLRKYSAYKNLRDSGHVVKTGFKFGTNFRVYRKVESIEKIPHSEYLVNAIPADFEFRLSVMSGAVRLANSVRKKMLFAVEKDEEIEYLDISRTKM

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Length
343 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
37.872 kDa
Sequence
MDGDLRGDTVHIGGDARQRFHDARGYGYPLGGNDIAVSLVEAAHLLFRGDLDSVDGMGFRAFLTDREAGFAARFLVYVDLRDRGFYLVPDRDPWWRDPGDGDFVVFPRGNTRRDGVVKHRVRVVDERTTLPVDGLSESVLAVVDEESEITYLDIAPETPTGETTLDRPTDVPGVLLDDRVLVWEPPQRLHNAGFYGQPLGGRAADHDALQLSLVEAAYLIDAGVLRLTDATIDDVRARGRLGEGEHFDCRLAVYRALRDAGAVPKTGFKFGADFRVYSAVSSVDDLGHSELLVRVITDTHVFSPGDLSLDVRLAHGVRKRMVFAATDDTDDTIRWLSVSRLTP

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
Length
343 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
37.872 kDa
Sequence
MDGDLRGDTVHIGGDARQRFHDARGYGYPLGGNDIAVSLVEAAHLLFRGDLDSVDGMGFRAFLTDREAGFAARFLVYVDLRDRGFYLVPDRDPWWRDPGDGDFVVFPRGNTRRDGVVKHRVRVVDERTTLPVDGLSESVLAVVDEESEITYLDIAPETPTGETTLDRPTDVPGVLLDDRVLVWEPPQRLHNAGFYGQPLGGRAADHDALQLSLVEAAYLIDAGVLRLTDATIDDVRARGRLGEGEHFDCRLAVYRALRDAGAVPKTGFKFGADFRVYSAVSSVDDLGHSELLVRVITDTHVFSPGDLSLDVRLAHGVRKRMVFAATDDTDDTIRWLSVSRLTP

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)
Length
339 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
37.418 kDa
Sequence
MQGRLEDGVVHLPGDARQRFHDSRGYGRPTGGDDLEVAPVEAAHLLSRDDIDGVDGMGLRELLARTGTTLDFVVYKDLRDRGFYLSPAREGWPGVADAADADFLVYPRGKGPWDGEVEHRVRVVGERESIPVSSLGEVVLAIVDEDGDLTYFDTEGDDPEGTAAEDLPADLDAELLSDRALVWDGVDRLYQRGFFGQRLYGRNADSGPLQLSLLEAAYLARADALAIDEADVVSRGRDVEGDRFDRRLAVYAALREAKTVPKSGFKFGSDFRVYTEFESVDDLSHSEFLVRVVAPDHTFVPRDLSLDVRLAGGVRKRMVFALTDDNGEIDWLSVSRLTP

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara)
Length
336 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
36.543 kDa
Sequence
MEGHLSDGLVEVGPNGRERYYDSSGYGRPDGAGVRLAPVEAAYLLARGDLDAVDGMGLEAFLADTPDLVVPFLVYRDLRERGFYVSPALEGWVADHAGIDFVVHPRGDGPWDGTVAYRVRVVDERGSVRLDSLGDVVLAVVDEESELTYLETDRPAVDGTSGLEADLTADGVLLEDRVLCWDAPPALYEQGFYGQRMDREDGPLQLSLLEAAHLAATGVLSVAGGAAAVEARGRDAEGERFDRRLTVYRTLRERGVVPKTGFKFGADFRTYADVDSVSELGHSELLVRVLPADATLSPRDVALDVRLAHGVRKRMVFALVDDGDEIRWVSVSRLTP

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)
Length
335 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
36.748 kDa
Sequence
MELTLEGDVVRAGYEARERFYDSRGYGKVRNGDIDLAPVEAAHLLYRGDIESIDGMDFRGFLGSAAVSEVDFAVYKDLRDRGFYLTPAREGWVDDAAGADFVVYPRGKGPWDNEVAYRVRIIGERDTAVATDLGGCVLAVVDEESEVTYLDTDRREVSGTSDAAVPATAGELLGERVVCWEPPAELYEQAFYGQQLGDDGAVQLSLVEAAYLTQEGMLTVEGGADAVVERGREVEGDRFDRRLAVYTALRASGVAPKTGFKFGADFRTYADVESAENLGHSELLVRVLPADHRFEPRDLALDVRLAHGVRKTMVFALTTDGGDEIEWVAVERLTP

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Length
305 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
35.959 kDa
Sequence
MIGGDFAVVKAKKSLERRGFGVKRGDKIYLHPLEVVYLQIKGIESFGELEDVLSWAESRMEDFSTYYFVYEDLRDRGNKVKIQGEFLLTKKPYLPISERKTIRMEEIAEKARNFDELRLAVVDEESEITYFRVYEPDMMGEQKEELPEIAGILSDEYVITKQTEIFSRYFYGSEKGDLVTLSLIESLYLLDLGKLNLLNADREELVKRAREVERNFDRRYEVYRNLKERGFVVKTGFKFGSEFRVYRKVESVDDLPHSEYLVDIADSREIRLIDLARAVRLAQNVRKRMVFAYGKNYLCFERVKV

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Length
292 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
33.923 kDa
Sequence
MSDGLCNGISFNVSEGHGPNYIVGRYKLGFVQQGILFLDPYECLYLYFKGRITFEDSEESALKKLFSITSVERYTAYTLLKNKGYRVHEDNGTIFFRKSTEIPRSVVVVREYDEIDVGKFFTERPDFYFTVDEEGDATIYRIDEIDIKGTENNKIGKCSVTDFGDRHFTKDDLPHWMGTKFHEYRMLTDFEANLIEGKEPKGMAEEVYRDLLSRGCIVKTGFKYGTNFRVYEGEKSEHAEYLVSVVENKLIWYYISRAVRVASSVRKKMIFTTIIDGKIKYIMISRSKDIVI

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Length
289 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
33.23 kDa
Sequence
MEQGICGSHVFFIEDGKSKNYIIGKYKIGYLSGDNLILDPYECLYLYFKGRISFQNSDSFRDLFDTVTFDRYVAYEILKNKGYRVKEDSGLIYFRKGTEKPLSLRVMREYDRIQFSDLVENPVDYYFTVDEEGDPTVYSSQEIFPAGRNLVSPVSAPVVRMGGRSFGAGDLEWWIGTAFHGFRLLTENEANYISGNHSASQVDMVYSDLVGRGCIVKTGFKYGANFRVYLGRDSQHAEYLVSVMPEEERWYSISRGVRVASSVRKTMIYASIYKNEVRYVALKRVKDII

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Length
284 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.
Mass
33.533 kDa
Sequence
MDYIIEDGFHFDIKNGKSPSYLINKYRTGHVIGNVYLLNKYEAFYLYLKNKISIDDEFFNGNIKFYMAYENLIGSGLYVKILNDCFMCRKSRNSRYKKVRFMPDDILLSFKDLYSDDSNIYITVDEEYESVYYSMERIDIKGSRKDDFSAASIDVSSGAYFGMNCPEWFGIDFHGKRLLNDYEIRFLNNDVKSNVDVIYKDLIKRGFIVKSGFKYGSNFRIYKNSMNEHSDYLVNYMDHDLWYVIARAVRLASNVRKRLIISGIIDNDPVYIKIERIKDIKTIL

Gene
endA
Protein
DNA-entry nuclease
Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Length
274 amino acids
Function
By degrading DNA that enters the cell, plays a role in the competence of cells to be transformed.
Similarity
Belongs to the DNA/RNA non-specific endonuclease family.
Mass
29.891 kDa
Sequence
MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Gene
endA
Protein
DNA-entry nuclease
Organism
Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
Length
274 amino acids
Function
By degrading DNA that enters the cell, plays a role in the competence of cells to be transformed.
Similarity
Belongs to the DNA/RNA non-specific endonuclease family.
Mass
29.891 kDa
Sequence
MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Length
186 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.602 kDa
Sequence
MGDRCAPIKASGVLIGDSVLVTDVEQARSLYSCGYYGQPLDVEKPRGADFEGPLRLSLIESLYLAEKGVLEVAKPDGSSVGVEDLRTAVRGNPRFSMLYNIYRDLRERGFVVRSGLKFGSDFAVYRLGPGIDHAPFIVHAYSPEDNIDPVEIVRAGRLSHSVRKKFVFAVTRGGDVSYLMIDWFRP

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Length
183 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.775 kDa
Sequence
MIGYLRGLAVIVEDVEFARRLYKEGFYGRFLGYDKVKRDEVEKINAPLILGLYEALYLAEKGRLKVMGEDGREVAPEELAALGRERMRNFDEIYKIYKYFRDLGYVVKSGLKFGALFSVYEKGPGIDHAPMVVVFLEPDKGISATDITRGGRLSHSVRKTWTLATVLRQTGEVVLLGFGWARL

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321)
Length
183 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.687 kDa
Sequence
MRGVLRGLAVVVEDVEFGRRLYKEGFYGRFLGYDKVKREEVDRINAPLVLALYEALYLVERGVLEVVTDSGEKITQTRLVELGREKLKNFDEIYKIYKYFRDLGYVVKSGLKFGALFSVYEKGPGIDHAPMVVVFLEPDKGISATDITRGGRLGHSVKKTFTLATVLKNTGEVILLGFSWARL

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)
Length
183 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.602 kDa
Sequence
MKGVLKGLAVVVEDVEFARKLYKEGFYGRFLGYDKVRREEVDKISAPLILALYEALYLAERGKLKVVSKNGTEVLPEKLIELGREKIKNFDDIYKIYKYFRDLGYVVKSGLKFGALFSVYERGPGIDHAPMVVVFLEPDRGISATDITRGGRLSHSVKKTFTLATVSKQTGEVVLLGFSWAKL

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Pyrobaculum neutrophilum (strain DSM 2338 / JCM 9278 / V24Sta)
Length
183 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.35 kDa
Sequence
MSGVLRGLAVVVQDVELARALYREGFYGRFLGYDKVKRDEVDKVSAPLILALYEALYLAERGRIKVVAEDGAEIPPEKLAELGRGMIRNFDDVYKIYKYFRDLGYVVKSGLKFGALFSVYEKGPGIDHAPMVVVFLEPDRGISATDITRGGRLGHSVKKTFTLATVLRQTGEVVLLGFSWAKL

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Length
182 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.71 kDa
Sequence
MVKALLVGSKVLVPSIDESRYLYSNGFYGKPIGISKPKGPKDIVRPLELSLIESVYLTKKGLINVVDKNGDLLEYKKLYEYSAMKINKFEILYKVYEDLREKGFIVRSGVKYGADFAVYTLGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTKKRLVLALVDRKSEGIRYIMFKWVKM

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Sulfolobus islandicus (strain M.16.27)
Length
182 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.798 kDa
Sequence
MVKALLVGSKVLIPNVDESRYIYSNGFYGKAIGISKPKGPKDIIRPLELSLIESVYLAKKGLIKVIDKNGEVLEYEKLYEYSSKIINKFDIMYRVYEDLREKGFIVRSGVKYGADFAVYTLGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTRKRLVLALVDRKSESVRYIMFKWVKM

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Sulfolobus islandicus (strain M.16.4 / Kamchatka #3)
Length
182 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.798 kDa
Sequence
MVKALLVGSKVLIPNVDESRYIYSNGFYGKAIGISKPKGPKDIIRPLELSLIESVYLAKKGLIKVIDKNGEVLEYEKLYEYSSKIINKFDIMYRVYEDLREKGFIVRSGVKYGADFAVYTLGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTRKRLVLALVDRKSESVRYIMFKWVKM

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Sulfolobus islandicus (strain L.S.2.15 / Lassen #1)
Length
182 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.856 kDa
Sequence
MVKALLVGSKVLIPNVDESRYIYSNGFYGKAIGISKPKDPKDIIRPLELSLIESVYLAKKGLIKVIDKNGEVLEYEKLYEYSSKIINKFDIMYRVYEDLREKGFIVRSGVKYGADFAVYTLGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTRKRLVLALVDRKSESVRYIMFKWVKM

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Sulfolobus islandicus (strain M.14.25 / Kamchatka #1)
Length
182 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.785 kDa
Sequence
MVKALLVGSKVLIPNVDESRYIYSTGFYGKAIGISKPKGPKDIIRPLELSLIESVYLAKKGLIKVIDKNGEVLEYEKLYEYSSKIINKFDIMYRVYEDLREKGFIVRSGVKYGADFAVYTLGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTRKRLVLALVDRKSESVRYIMFKWVKM

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2)
Length
182 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.842 kDa
Sequence
MVKALLVGSKVLIPNVDESRYIYSNGFYGKAVGISKPKDPKDIIRPLELSLIESVYLAKKGLIKVIDKNGEVLEYEKLYEYSSKIINKFDIMYRVYEDLREKGFIVRSGVKYGADFAVYTLGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTRKRLVLALVDRKSESVRYIMFKWVKM

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1)
Length
182 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.856 kDa
Sequence
MVKALLVGSKVLIPNVDESRYIYSNGFYGKAIGISKPKDPKDIIRPLELSLIESVYLAKKGLIKVIDKNGEVLEYEKLYEYSSKIINKFDIMYRVYEDLREKGFIVRSGVKYGADFAVYTLGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTRKRLVLALVDRKSESVRYIMFKWVKM

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)
Length
181 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.892 kDa
Sequence
MIRAVLLGLRAVVFDREAARRLYASGYYGKPLGIPKPRGSNFDAPLELGLLETLHLLERGMLEVYDEEGHRVSVEEVRRRAERFIPKFELLYKVYRDLRDRGYVVRSGLKYGSDFAVYERGPGLEHAPYLVHVMRVDEEIDPLEIVRAGRLSHSVRKAFILALTGPRDTPIRYLLLKWSKP

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Length
181 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.891 kDa
Sequence
MIQGEILGDKVLINDVEKAKEIYKLGYYGKPLDITKPKSPEDIKKPLILSLIEALYLSKKKIIEVIRDGKVIDDALLYKIGMENIPRFELLYTVYEDLREKKFVVRSGMKYGADFAIYTVAPGLEHAPYLVIAIDEEQEISPNEILGFGRVSHSTRKNLILSIVNQRNRSIRYIMFKWLKL

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Length
180 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.507 kDa
Sequence
MIGELVKDKILIKNIEDARLIYKMGYYGKPIGISKPKSAEEINSELILSLIEGVYLVKKGKLEIVSNGERLDFERLYQIGVTQIPRFRILYSVYEDLREKGYVVRSGIKYGADFAVYTIGPGIEHAPYLVIALDENSQISSNEILGFGRVSHSTRKELILGIVNLTNGKIRYIMFKWLKM

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Length
179 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.664 kDa
Sequence
MVRDKMGKKITGLLDGDRVIVFDKNGISKLSARHYGNVEGNFLSLSLVEALYLINLGWLEVKYKDNKPLSFEELYEYARNVEERLCLKYLVYKDLRTRGYIVKTGLKYGADFRLYERGANIDKEHSVYLVKVFPEDSSFLLSELTGFVRVAHSVRKKLLIAIVDADGDIVYYNMTYVKP

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Length
179 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.939 kDa
Sequence
MLCAGNGGKELPRAKVFEGGSLVSKDYEDLKRRYFGTEHGNVLFLDPFETVYLTEKGEIDPETPEGEPMSVEELLSFFERRRPGFRAGYVVYRDLTERGYVVKSGFKYGGRFRVYEEDPDREHSKYVVRVVEPDTELSTRDVLRATRLAHSVRKDFVLAVVEDVEEPRIEYVMWRWKRL

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Methanococcus maripaludis (strain S2 / LL)
Length
177 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.731 kDa
Sequence
MMAKPKKTIPAKLSDERIVIYDKDGISRLNEKRYGELHENFLSLSFVEGLYLVSKNWISLRDKNKKLLSFEELFDVAQNIDRKLCIRYLAYKDLRNRGYTVRTGLKYGSDFRLYERSNIDEIHSRYLVKVFSEEIPCEISEITGFVRVAHSVRKELIIAIVDADGSVVYYNMGYLKL

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)
Length
177 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.714 kDa
Sequence
MMAKPKKTIPAKLSDERIVIYDKDGISRLNEKRYGELHENFLSLSYVEALYLVAKDWVLIRDKKNKILSFEELHEIAHVIDKKLCIRYLVYKDLRNRGYTVRTGLKYGSDFRLYERSNIDEVHSKYLVKVFSEEIPCEISEITGFVRVAHSVRKELVIAIVDADGSVVYYNMGYLKL

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Thermococcus onnurineus (strain NA1)
Length
171 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.286 kDa
Sequence
MKEPIEFKLSGDRAFSEREKAINQLYNRRYFGEVVNGKLFLSLIEAAYLMERGKIKVLDGGKELSFEELFELGRKKDDQFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRRGMDEHSQWLIWVVPENLRFSANDITARVRVAHGVRKNMVMAVVDEDNDVVYYKIEWVKF

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Length
170 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
19.693 kDa
Sequence
MRVEGQLGDEVVTIKATSIARRLHGKSHYGKMYEDRLQLSLIEAAYLMERGKLKLMKDDDEVSPEEFISLLGERGLYSKYLVYRDLRNRGYIVKTGFKYGAEFRLYERGGAPGRTHSAYLVRVISENDTIHALDFSSYVRVAHGVNKKLLMAFLDDEEDITYYLVDWIRP

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Length
170 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.36 kDa
Sequence
MKKVIEFYLSGDRVYSTREKAINQLYNNRGYGELKGNKLFLSLIEAAYLVERGWIKVLDEDRELTFEEIFKLGKRKDEDFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRKNAEHSDWLIWVLRESEKLSPNDMTARVRVAHGVRKNMVMAIVDEDNDVVYYKIEWIKF

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Length
170 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
19.921 kDa
Sequence
MKTVIEFYLSGDRVYSEREKAINQLHINRGYGELKGKRLFLSLIEAAYLLEKGWIKVLDGERELSFYDVVSLGKKKDEDFDVKYLVYKDLRDRGYIVKSALKFGSHYRVYRKGAEHSDWLVWVVRESQKLSPNDITARARVAHGVRKTMVLAVVDEDGDVVYYKVEWTKF

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Length
170 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
20.168 kDa
Sequence
MKKPIEFYLSGDRVYSTREKAINQLYNNRGYGELKGDKLFLSLIEAAYLTEKGWIKVVDKDKELKFDDLMKLGKSRDEDFDIKYIVYKDLRDRGYIVKSALKFGSHYRVYRKDAEHSDWLIWVLRENQRLSPNDITARARVAHGVRKNMVLAIVDEDGDVVYYKVEWIKF

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)
Length
168 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
19.8 kDa
Sequence
MIVFYLSGDRVFSTDQNAINGLYNKRHFGKLVEGKLFLSLLEATYLVERGKIEVREGKRKLTVEELMNLGRERDELFDAKYLVYKDLRDRGYTVKSGLKFGSHFRVYRRGMDEHSQWLVWVLPENSRLSPNDITARVRVAHGVRKNMIMAIVDEDADVTYYKVEWVRF

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
Length
168 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
19.697 kDa
Sequence
MIIFYLSGDRVFSTDQNAINGLYNKRYFGKVVEGKLFLSLLEAAYLVERGKIEVRDGKRKLSLEEIMNLGRARDELFDAKYLVYKDLRDRGYTVKSGLKFGSHFRVYRRGMEEHSEWLVWVVPENSRLSPNDITARVRVAHGVRKNMIMAIVDEDADVTYYKVEWVKF

Gene
endA
Protein
tRNA-splicing endonuclease
Organism
Nanoarchaeum equitans (strain Kin4-M)
Length
154 amino acids
Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Similarity
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.
Mass
18.254 kDa
Sequence
MIGYLFGNRVLVDDKELPLIEAYYLLDKGELEVYEDDKKLSKEEFLKKCLTYDERFLIRYKAYKELRDKGYTLGTALKFGADFRVYDIGVIPKKGKRSEREHSKWVLYPVSKDETFDFYEFASKNRVAHSTRKKLLLGIVSDKIEFIEVSWKKP