Protein
Type IIS restriction enzyme Eco57I
Function
Recognizes the double-stranded sequences CTGAAG and CTTCAG and cleaves respectively 22 bases after C-1 and 14 bases before C'-1. Also acts as a methylase that causes specific methylation on A-5 on one strand, the other strand being methylated by the Eco57IB methylase.
Sequence
MNKKDQLQRLIDKYKSDIDYYRSARYNETQLRTDFLDQLFLILGWDITNAAGKPTNEREVLVEEGLKARAGENTKKPDYTFRLFSERKFFLEAKKPSVDISTTIEPALQVRRYGFTAKLKISVLSNFEYTAIYDCSNQVKETDSVANSRIKLYHFTELVDKFDEINNLIGRESVYTGHFDNEWSEIENKILRFSVDDLFLKQINDWRLLLANEFLQIKNELPEEKLNDLVQNYINSIVFLRVCEDRDLEEYETLYHFAQDKDFQSLVKKLKSSDKKYNSGLFSLEYIDELLSNANSCIWSIIEQLYFPQSTYSFSVFSSDILGNIYEIFLSEKVRIDELGNVKIQPKEEHIDRDVVTTPTHIVKEIIRNTVVEYCKGKSDIEILNSKFADIACGSGAFIIEAFQFIQDILIDYYIQNDKSKLQQISEHTYKLKFEVKREILCKCIYGIDKDYNATKACTFGLLLKLLEGETTETIGKDTPILPALDTNILFGNSLIDSGDKVKQEDIFSINPFDLTNYQFDVIVGNPPYMATEHMNQLTPKELDIYKRKYKSAYKQFDKYFLFIERSIQILKEYGYLGYILPSRFIKVDAGKKLRKFLSENKYLSKLISFGSHQVFKNKTTYTCLLFLNKENHDNFSFYEVKDFKKWLTREDKYLLSSTYQTSSLDSDTWVLEKKINDILKLMFSKSEQLGNIVGKSNVANGIQTSANKYYIHKEIKSENGFIYFEYDGIEYHIEKELTRPYFETNRSGDDSFYTYKDVEPNSFVVYPYKKVGERIQFIEYDELKRQYPKLFEFLQVVKVHLNDKKRSIKPDPTGPNEWYRYGRSQALENCDVDQKLIVGILSNGYKYSIDNHRTFVSSGGTAGYSIINIPNNVRYSIYYIQAILTSKYLEWFASIYGDIFRGRFVARGTKVQTRMPIPTIDFDDPKQKEIHDTISSKQQYLNKLYSQTQKSADRDKIIFERQFEQEKIQMDYLIKNLFDLGDLDSEIPTVEDLYKNL