Protein
tRNA-dihydrouridine(47) synthase [NAD(P)(+)]
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Function
Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs (By similarity).
Similarity
Belongs to the Dus family. Dus3 subfamily.
Sequence
MESQETAKRPIEPNGAVAAQDAEPATKRVKLDDAPVPQIQEEPSQPQPQPQPQPQTEDEKPTEQRQDDRDKRRGIAPIKKEYLVVPPSQVAKTAEVVDDDAAEGRTAPGAEGAAGKDGKKKRPKGQNKEREFGIFADAQRLCNSVAWTPEFSPRHCKHGERCNALHDIRKYLKEGRRPDLNVFGGKCPVWETHGKCSSGWRCLFVESHMKEIEHEDGRKELVLTEDPTKIKPEAAPLVPPEEESMDARAGVYNVVAPAVKLALSRKKIQHEKSDQYLKWMAKDSELARIHYHKQKDDDDVAKDYAAQYVEPPLKPSEKRRIYFGRETPVLAPLTTQGNLPFRRLCVELGAEITYSEMALGLPLLQGQKADWTLMRAHESEITPPRYTPTGIVDPAYDNSKDLKFGVQITAHAPWIAIKSAETLARYLPHLRVIDLNCGCPIDMVYKSGAGSALLDAPSKLERMIRGMNTVSGEVPVTAKIRMGVRDNHLTAQKLVERLALGAPDIRAVSGAPGCAAVTLHGRTRQQRYTKAADWSYIAECAALVKSFNEKADSLQDTIREADERSLPNGGRMYFVGNGDCYSHVDYFNHVDNAKVDSVMVGRGAIIKPWIFEEISAGQYLDKSATERLAYVEKFAKYGMEAWGSDELGLNYTRRFLLEFLSFFHRYVPIGLLEYLPPAMNDRPPAYKGRNELETLLASKNYLDWIKISEMFLGPAPPNFKFQPKHKSNAYENIEAEG