About Products Protein Database Contact

dpkA

Gene
dpkA
Protein
Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2-carboxylate reductase
Organism
Pseudomonas syringae pv. tomato
Length
343 amino acids
Function
Catalyzes the reduction of both Delta(1)-pyrroline-2-carboxylate (Pyr2C) and Delta(1)-piperideine-2-carboxylate (Pip2C) to L-proline and L-pipecolate, respectively, using NADPH as the electron donor. Can catalyze the reverse oxidation reactions, albeit at a much lower rate. Is also able to catalyze in vitro the NADPH-dependent formation of N-methylalanine from pyruvate and N-methylamine; can act on other alpha-keto acids and specifically uses methylamine and not ammonia for these reductive amination reactions. Can use NADH instead of NADPH, although with much less efficiency.
Similarity
Belongs to the LDH2/MDH2 oxidoreductase family.
Mass
36.249 kDa
Sequence
MSASHADQPTQTVSYPQLIDLLRRIFVVHGTSPEVADVLAENCASAQRDGSHSHGIFRIPGYLSSLASGWVDGKAVPVVEDVGAAFVRVDACNGFAQPALAAARSLLIDKARSAGVAILAIRGSHHFAALWPDVEPFAEQGLVALSMVNSMTCVVPHGARQPLFGTNPIAFGAPRAGGEPIVFDLATSAIAHGDVQIAAREGRLLPAGMGVDRDGLPTQEPRAILDGGALLPFGGHKGSALSMMVELLAAGLTGGNFSFEFDWSKHPGAQTPWTGQLLIVIDPDKGAGQHFAQRSEELVRQLHGVGQERLPGDRRYLERARSMAHGIVIAQADLERLQELAGH

Gene
dpkA
Protein
Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2-carboxylate reductase
Organism
Pseudomonas putida
Length
341 amino acids
Function
Catalyzes the reduction of both Delta(1)-pyrroline-2-carboxylate (Pyr2C) and Delta(1)-piperideine-2-carboxylate (Pip2C) to L-proline and L-pipecolate, respectively, using NADPH as the electron donor. Can use NADH instead of NADPH, although with much less efficiency. Plays an essential role in the catabolism of D-proline and D-lysine, which allows P.putida to grow on each of these amino-acids as a sole carbon source; D-lysine appears to be catabolized only through the pipecolate pathway. Can also catalyze the reverse oxidation reactions, albeit at a much lower rate. To a lesser extent, is able to catalyze in vitro the NADPH-dependent formation of N-alkyl-L-amino acids from the corresponding alpha-oxo acids and alkylamines, e.g. the formation of N-methylalanine from pyruvate and N-methylamine; cannot use ammonia as substrate for these reductive amination reactions. Shows neither malate dehydrogenase nor lactate dehydrogenase activity.
Similarity
Belongs to the LDH2/MDH2 oxidoreductase family.
Mass
35.973 kDa
Sequence
MSAPSTSTVVRVPFTELQSLLQAIFQRHGCSEAVARVLAHNCASAQRDGAHSHGVFRMPGYVSTLASGWVDGQATPQVSDVAAGYVRVDAAGGFAQPALAAARELLVAKARSAGIAVLAIHNSHHFAALWPDVEPFAEEGLVALSVVNSMTCVVPHGARKPLFGTNPIAFAAPCAEHDPIVFDMATSAMAHGDVQIAARAGQQLPEGMGVDADGQPTTDPKAILEGGALLPFGGHKGSALSMMVELLAAALTGGHFSWEFDWSGHPGAKTPWTGQLIIVINPGKAEGERFAQRSRELVEHMQAVGLTRMPGERRYREREVAEEEGVAVTEQELQGLKELLG