Protein
Diphthine synthase
Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Function
S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.
Similarity
Belongs to the diphthine synthase family.
Sequence
MLYIIGLGLYDENDISIKGLRAIEDCDEVYAEFYTAMLQGSSLSAIERRIGRSVKVLGRGEIEEERIPIERALELDVALLVAGDPLVATTHTELLIDAHRRGIETRVIHSSSIISAAPGLAGLQAYKFGRIITIPFTSDNYFPTSPYTNIGENLKAGSHSLVLLDIEAHRNRYMTATEGLRYLLKASEKLDDGTISGETLAVVIARAGSEKPLVRADKIATLVEEDFGDPLHCLVVPAGLHFIEADYLVEIAGAPRAVVERMIL