Protein
Decapping nuclease dom-3
Organism
Caenorhabditis elegans
Function
Ribonuclease that specifically degrades pre-mRNAs with a defective 5' end cap and is part of a pre-mRNA capping quality control. Has decapping and pyrophosphohydrolase activities. Has decapping activity toward incomplete 5' end cap mRNAs such as unmethylated 5' end-capped RNA to release GpppN and 5' end monophosphate RNA. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5' end triphosphate to release pyrophosphates (By similarity).
Similarity
Belongs to the DXO/Dom3Z family.
Sequence
MSHYGGNPRGNSSHQFGRKDFQQSDSKHIPKITGQPLPNEVQIPFDNMIYETRNPPKFEKQAKFISEYCINYDRKLQLGRMRAKKFHDKLPPNNLSLDLGKGFETFDPKEGDEKIIMLLEWINQMAPEGGRLKKVIHEADVVCWRGLITKICSTIYNKEEGWRIDAIKRKGVIFLCEEKTEQAKNRDLNQTDLDKRMSYWGHKFEQYVTLDDDAEQPDTSSPVTTKEEYAVVFRNDLQTDQRLNPEKRSIGIFYSGEVDCLDRHGNMLELKTQKGELGHGFYKYSKSFKWWLQSSLVNVDHIIVGLRTQEGHVKSLTSLRTREIPQRASWNFRAGFEFLSTIFTYILNCLEKDGDACVIEYRSEMGIHKGIQMRRVPVDEFDFVPNEFLEKFC