Protein
Cobalamin adenosyltransferase
Organism
Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)
Function
Adenosyltransferase that catalyzes the conversion of cob(II)alamin to adenosylcob(III)alamin (AdoCbl) in the presence of ATP and an electron donor. Acts as an accessory protein of IcmF that functions in cofactor repair, since IcmF is prone to inactivation during catalytic turnover due to the occasional loss of the 5'-deoxyadenosine moiety and formation of the inactive cob(II)alamin cofactor in its active site. Thus, receives and repairs the inactive cofactor, which is then reloaded onto IcmF in a GTPase-gated step.
Similarity
Belongs to the Cob(I)alamin adenosyltransferase family.
Sequence
MGNRLSKIATRTGDAGTTGLGDGSRVGKNSLRIVAIGDVDELNSHIGLLLTEPDLPEDVRAALLHIQHDLFDLGGELSIPGYTLLKAPQVAQLDDWLAHYNAALPRLAEFILPGGSRPAAQAHICRTVCRRAERALVELGAAEALNEAPRQYLNRLSDLLFVLARVLNRAGGGSDVLWQRERES