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cmr5

Gene
cmr5
Protein
CRISPR system Cmr subunit Cmr5
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Length
169 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA (target NRA), generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. Cmr4 is probably the subunit that cleaves target RNA (PubMed:25280103). Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not absolutely necessary for target cleavage (PubMed:19945378).
Similarity
Belongs to the CRISPR system Cmr5 family.
Mass
19.687 kDa
Sequence
MEVHMLSKDNKKSIRKTLEQRRGEYAYYVIKEVADLNDKQLEEKYASLVKKAPVMILSNGLLQTLAFLLAKAETSPEKANQILSRVNEYPPRFIEKLGNDKDEHLLLYLHIVYWLRENVDRNIDVKTLLSQDYSKVLWATKEAIALLNWMRRFAVAMLKEEGKENEGSS

Gene
cmr5
Protein
CRISPR system Cmr subunit Cmr5
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Length
169 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA (target NRA), generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. Cmr4 is probably the subunit that cleaves target RNA (PubMed:25280103). Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not absolutely necessary for target cleavage (PubMed:19945378).
Similarity
Belongs to the CRISPR system Cmr5 family.
Mass
19.687 kDa
Sequence
MEVHMLSKDNKKSIRKTLEQRRGEYAYYVIKEVADLNDKQLEEKYASLVKKAPVMILSNGLLQTLAFLLAKAETSPEKANQILSRVNEYPPRFIEKLGNDKDEHLLLYLHIVYWLRENVDRNIDVKTLLSQDYSKVLWATKEAIALLNWMRRFAVAMLKEEGKENEGSS

Gene
cmr5
Protein
CRISPR system CMR subunit Cmr5
Organism
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Length
156 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). The CMR complex degrades RNA complementary to the crRNA (target RNA) within UA dinucleotides, generating 3'-OH and 5'-phosphate ends. Activity is dependent on the 8 nt long 5' tag in the crRNA, an unpaired 3' flag on the target RNA, and is stimulated by ATP. Some cleavage of the guide crRNA can also be observed.
Similarity
Belongs to the CRISPR system Cmr5 family.
Mass
18.145 kDa
Sequence
MTEVQEEKDYKLAIEAFNLVYNALDKKAREKFRSRARDMVEEIYTSGFIPTLFYIISKAGLENNNDKLTALLNSPSSKSIDLGSSDEASYMAYLFVILYYLSERNIINKEFLINKLKNNRLELIDKLYELSPIISARIKRYLMAIKRLAEALIEVR

Gene
cmr5
Protein
CRISPR system CMR subunit Cmr5
Organism
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Length
156 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). The CMR complex degrades RNA complementary to the crRNA (target RNA) within UA dinucleotides, generating 3'-OH and 5'-phosphate ends. Activity is dependent on the 8 nt long 5' tag in the crRNA, an unpaired 3' flag on the target RNA, and is stimulated by ATP. Some cleavage of the guide crRNA can also be observed.
Similarity
Belongs to the CRISPR system Cmr5 family.
Mass
18.145 kDa
Sequence
MTEVQEEKDYKLAIEAFNLVYNALDKKAREKFRSRARDMVEEIYTSGFIPTLFYIISKAGLENNNDKLTALLNSPSSKSIDLGSSDEASYMAYLFVILYYLSERNIINKEFLINKLKNNRLELIDKLYELSPIISARIKRYLMAIKRLAEALIEVR

Gene
cmr5
Protein
CRISPR system Cmr subunit Cmr5
Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Length
155 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Part of the Cmr ribonucleoprotein complex (By similarity).
Similarity
Belongs to the CRISPR system Cmr5 family.
Mass
17.388 kDa
Sequence
MDIREIEQERASFAFKVVSDIKDKYSQNKKVQGKYSSYAEKAPTIILNNGLGATLAFFLSKLEKPIDDVDYKSINPESFGNAENIAYAFLYKHLSTWLAEGNGKDSAFSGLTNGEDPLKYIMEKTAIDVAISTEEALSILNWIKKFAKAMLEEEL

Gene
cmr5
Protein
CRISPR system Cmr subunit Cmr5
Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Length
155 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Part of the Cmr ribonucleoprotein complex (By similarity).
Similarity
Belongs to the CRISPR system Cmr5 family.
Mass
17.388 kDa
Sequence
MDIREIEQERASFAFKVVSDIKDKYSQNKKVQGKYSSYAEKAPTIILNNGLGATLAFFLSKLEKPIDDVDYKSINPESFGNAENIAYAFLYKHLSTWLAEGNGKDSAFSGLTNGEDPLKYIMEKTAIDVAISTEEALSILNWIKKFAKAMLEEEL

Gene
cmr5
Protein
CRISPR system Cmr subunit Cmr5
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Length
129 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Part of the Cmr ribonucleoprotein complex (By similarity).
Similarity
Belongs to the CRISPR system Cmr5 family.
Mass
14.572 kDa
Sequence
MGTCLEVMKMKTLSLELAKLAMNLVEEVEKDKRDDYLRDVKGLGSMIVQNGLMGTILFLKKKGKNKVVDHLGKMIEHLTLEDGIVERLMENEFLDARTYLKAQIAALECAKWLKRCGEVLLGGEENETT

Gene
cmr5
Protein
CRISPR system Cmr subunit Cmr5
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Length
129 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Part of the Cmr ribonucleoprotein complex (By similarity).
Similarity
Belongs to the CRISPR system Cmr5 family.
Mass
14.572 kDa
Sequence
MGTCLEVMKMKTLSLELAKLAMNLVEEVEKDKRDDYLRDVKGLGSMIVQNGLMGTILFLKKKGKNKVVDHLGKMIEHLTLEDGIVERLMENEFLDARTYLKAQIAALECAKWLKRCGEVLLGGEENETT

Gene
cmr5
Protein
CRISPR system Cmr subunit Cmr5
Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Length
114 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Part of the Cmr ribonucleoprotein complex (By similarity).
Similarity
Belongs to the CRISPR system Cmr5 family.
Mass
12.961 kDa
Sequence
MRTRSQVWAQKAYEKVREAAKGEGRGEYRDMALKLPVLVRQAGLSQALAFVDSRGKEAHKALGNDLAQVLGYRDLRELAEAAREAELLQYLRLTREVLAAAEWFKRFAQALIEE

Gene
cmr5
Protein
CRISPR system Cmr subunit Cmr5
Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Length
114 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Part of the Cmr ribonucleoprotein complex (By similarity).
Similarity
Belongs to the CRISPR system Cmr5 family.
Mass
12.961 kDa
Sequence
MRTRSQVWAQKAYEKVREAAKGEGRGEYRDMALKLPVLVRQAGLSQALAFVDSRGKEAHKALGNDLAQVLGYRDLRELAEAAREAELLQYLRLTREVLAAAEWFKRFAQALIEE