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cas6f

Gene
cas6f
Protein
CRISPR-associated endonuclease Cas6/Csy4
Organism
Pseudomonas aeruginosa (strain UCBPP-PA14)
Length
187 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Processes pre-crRNA into individual crRNA units. Absolutely required for crRNA production or stability. Upon expression in E.coli endonucleolytically processes pre-crRNA, although disruption and reconstitution experiments indicate that in situ other genes are also required for processing. Yields 5'-hydroxy and 3'-phosphate groups. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.
Similarity
Belongs to the CRISPR-associated endoribonuclease Cas6 family. Cas6f/Csy4, subtype I-F/Ypest subfamily.
Mass
21.391 kDa
Sequence
MDHYLDIRLRPDPEFPPAQLMSVLFGKLHQALVAQGGDRIGVSFPDLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQAKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGPLQVTAEEGGFTCYGLSKGGFVPWF

Gene
cas6f
Protein
CRISPR-associated endonuclease Cas6/Csy4
Organism
Pseudomonas aeruginosa (strain UCBPP-PA14)
Length
187 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Processes pre-crRNA into individual crRNA units. Absolutely required for crRNA production or stability. Upon expression in E.coli endonucleolytically processes pre-crRNA, although disruption and reconstitution experiments indicate that in situ other genes are also required for processing. Yields 5'-hydroxy and 3'-phosphate groups. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.
Similarity
Belongs to the CRISPR-associated endoribonuclease Cas6 family. Cas6f/Csy4, subtype I-F/Ypest subfamily.
Mass
21.391 kDa
Sequence
MDHYLDIRLRPDPEFPPAQLMSVLFGKLHQALVAQGGDRIGVSFPDLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQAKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGPLQVTAEEGGFTCYGLSKGGFVPWF

Gene
cas6f
Protein
CRISPR-associated endonuclease Cas6/Csy4
Organism
Escherichia coli (strain UTI89 / UPEC)
Length
184 amino acids
Function
Complements a csy4 disruption in Pseudomonas aeruginosa PA14, restoring inhibition of biofilm formation and generation of crRNA.
Similarity
Belongs to the CRISPR-associated endoribonuclease Cas6 family. Cas6f/Csy4, subtype I-F/Ypest subfamily.
Mass
20.72 kDa
Sequence
MDHYLEIRVLPDPEFSSEMLMAALFAKLHRVLGARGQGDIGVSFPDVNVMPGARLRLHGSAQALQALEASTWRKGLTDYCQCSPVTPVPEIKGWRVVSRVQVKSNPQRLLRRSVKKGWLTEEQAIERLATQAEQRTDLPFLNMKSLSSQQLFKLFIRHGDLLKEPVKGEFSSYGLSATATIPWF

Gene
cas6f
Protein
CRISPR-associated endonuclease Cas6f/Csy4
Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)
Length
184 amino acids
Function
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Processes pre-crRNA into individual crRNA units.
Similarity
Belongs to the CRISPR-associated endoribonuclease Cas6 family. Cas6f/Csy4, subtype I-F/Ypest subfamily.
Mass
20.459 kDa
Sequence
MDHYIDIRVQPDPEFTASQLLNALFAKLHRVLGQLANGKIGISFPEVGKTLGECLRLHGTEDALSTLEKTSWLKGLRDYTQVSECKVVPNGVKFRTVRRVQLKSSAERLRRRSVSKGWLTAAEAAARIPDAVEKRSALPFVQIKSLSNGQMFFVFVEHGPLQNAPTAGRFSSYGLSTEATVPWF