Protein
Manganese-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase
Organism
Bacillus sp. (strain JF8)
Function
Catalyzes the meta-cleavage of the hydroxylated biphenyl ring. The enzyme can oxidize a wide range of substrates, and the substrate preference order is 2,3-dihydroxybiphenyl > 3-methylcatechol > catechol > 4-methylcatechol > 4-chlorocatechol.
Similarity
Belongs to the extradiol ring-cleavage dioxygenase family.
Sequence
MTAEIAKFGHIALITPNLEKSVWFFRDIVGLEEVDRQGDTIFLRAWGDWEHHTLSLTPGNRARVDHIAWRTKRPEDVETFAEQLKAKGTEVQWIEPGEEKGQGKAIRFRLPNGYPFEIYYDVEKPKAPEGKKSRLKNNVYRPSYGIAPRRIDHVNVWTTNPSEIHQWLKDNMGFKMREYIRLNNGFVAGGWMSVTPLVHDIGVMVDPKGQPNRLHHFAYYLDNVTDILRAADILREHDITIEMGGPGRHGISQAFFLYVKDPGSGHRLELFSGGYLIFDPDWEPIEWQEHELQEGLIWYGPEMKPGGPMDDTTEC