upf1

Regulator of nonsense transcripts 1

1331 amino acids

Eliminates the production of nonsense-containing RNAs.

Belongs to the DNA2/NAM7 helicase family.

151.558 kDa

MYGFNIGKAENAGLHLDNKKSENNNSSNNELHKVINTINNGFQFKDDDDEDNENNLNNESSSDEENQQQQNNNNNNNKNQFEDDDEDQEEEEYISGDDLNNNGQESDDYEEEEEDDDEDEEEEDEESKKLNYDSFFGANMSHNMDFLNSQNINSQIGLMDPHLHSSQLNFEEPQEEIELPAHACAYCATHELSTVVKCMHPSCGKWFCNGKGKTKSSHIITHLVKSKHKEVALHPESSFGDTTLECFNCGCKNIFLLGFITARTESVVVLLCRDPCASGPSKEVNWDMSSWQPLINGGEKAFCSWLVKTPSQVDSERSRQITIQQILRLEEFWKMDPEATLLDIEAPRSDDEKPASTQLAYKDAYEYREIISPLIELEAKHEKELRESLSQSGISIEWSQGINKRYTATFPFSRSDLEFKVVPGDELKLQFISSTGGVIEWEDTGRVIHIDDENLLSLETKSRCSFDSGPKGSYRMEMVWRSTSSERILSAMKSFAIKEQALSSYLYHALLGHPDIPPAPLDIQLPTNFHLKNLPRLNESQISAVNKVLTAPLSLIQGPPGTGKTVISSFIIHHLVKYVKGNDKVLVCTPSNVAIDQLTGKLHEIGLKVVRLSSKLREEVASPVEHLTLHKQVYKLDQMGDGELGKLRKLKEAFGSLSNEDEKRYIYLRRMMEMAILRKADVICATCVGAGDPRLSQFRFPHILIDESTQASEPECLIPLMMGAKQVILVGDHRQLGPVLLCKKVVDAGLSQSLFERLISLGHHPERLTIQYRMHPSLTEFPSNTSYEGQLVSELSHTDRDSQSKFPWPQPKDPMFFFNCTGSEEISSSGTSFINTTEASICEKIVTKFLELGSLPGQIGIITPYEGQRAYITSHMQKSGKLNLELYKSIEVASVDSFQGREKDYIILSCVRSNDYQGIGFLQDPRRLNVALTRARFGLIILGNAKVLSKDPLWNSLISHFKNKNVLVEGSLANLKQSPVILQKPKKLYGQGKLPIPGQNSNSFNYDREHIDPNIGMNMVYGISNNNINNNSNNINNNNNNNINNNINNNNNQRFNTQDGRYSNSQTSSSSQTYYGSTNSSGNTINSNQNQFFNTPSSYSANTGSSYQYRFQQYQQLQQPQPQQQQQPQQQQQPQQQQQPQQPQQQQPQQQKQYQQQQQQQQQQQQQQQQQQQQQKQQPHQQYQSQKQQQQQQYQQPQQYQQQNQQYHQSQLPPKQYHNQRFNNNNNNNINNNNNNNNNNNNNNNNNNNNNNINNNNSKNQNQNQSPRRSPVVGTLQLHCKFDSQPNNNKHISDPNQPQISNPNMSIPLSQSLSFNDLSQEQFNKSNSRKD

UPF1

Regulator of nonsense transcripts 1 homolog

1254 amino acids

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (premature termination codon PTC) by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Eliminates the production of nonsense-containing RNAs (ncRNAs). Required for plant development and adaptation to environmental stresses, including plant defense and response to wounding.

Belongs to the DNA2/NAM7 helicase family.

136.868 kDa

MDSQQSDLFDTASQPDTVADEYTFLEFNTQGDSEFDYQDFGSPTAWPTPSDSISIADVADRGEGGAAADHHSEASSPSSLSAGAGNGAKVGRGGVGGSGGVSSSSQVDALAAGVGNLNFEETGDDDGFDYGKNDFTEHACKYCGISNPACVVRCNVASCRKWFCNSRGNTSGSHIVNHLVRAKHKEVCLHRDSPLGETILECYNCGCRNVFLLGFISAKTDSVVVLLCRDPCLNVNALKDMNWDLSQWCPLIDDRCFLPWLVKVPSEQEQLRARQISAQQINKIEELWKTNPDATLEDLEKPGVDDEPQPVQPKYEDAYQYQNVFAPLIKLEADYDKMMKESQSKENLTVRWDIGLNKKRVAYFVFPKEENELRLVPGDELRLRYSGDAVHPSWQSVGHVIKLTAQEEVALELRANQGVPIDVNHGFSVDFVWKSTSFDRMQGAMKNFAVDETSVSGYIYHQLLGHEVEAQMVRNTLPRRFGVPGLPELNASQVNAVKSVLQKPISLIQGPPGTGKTVTSAAIVYHMAKQGQGQVLVCAPSNVAVDQLAEKISATGLKVVRLCAKSREAVSSPVEYLTLHYQVRHLDTSEKSELHKLQQLKDEQGELSSSDEKKYKNLKRATEREITQSADVICCTCVGAADLRLSNFRFRQVLIDESTQATEPECLIPLVLGVKQVVLVGDHCQLGPVIMCKKAARAGLAQSLFERLVTLGIKPIRLQVQYRMHPALSEFPSNSFYEGTLQNGVTIIERQTTGIDFPWPVPNRPMFFYVQLGQEEISASGTSYLNRTEAANVEKLVTAFLKSGVVPSQIGVITPYEGQRAYIVNYMARNGSLRQQLYKEIEVASVDSFQGREKDYIILSCVRSNEHQGIGFLNDPRRLNVALTRARYGIVILGNPKVLSKQPLWNGLLTHYKEHECLVEGPLNNLKQSMVQFQKPRKIYNDRRLFYGGGAGMIGNDNFGSGNPNADRRGSRGRAGGSYLPSGPPNGARPGLHPAGYPIPRVPLSPFPGGPPSQPYAIPTRGPVGAVPHAPQPGNHGFGAGRGTSVGGHLPHQQATQHNVGTIGPSLNFPLDSPNSQPSPGGPLSQPGYGSQAFRDGFSMGGISQDFLADDIKSQGSHDPYNMADFATQASPGGFAVDYATQGAHGAFPGNFMNQNSQGGYSRFSGINDFMSQEYMAHGGQGLFTQAGFIDSSQDDGQQNPYGVNNPNLQSQGLPNSLYSQPFAHYNTQPLNLSGPQQSQPNQSSQNPKHPYNG

Upf1

Regulator of nonsense transcripts 1 homolog

1180 amino acids

RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. The formation of an Upf1-Upf2-Upf3 surveillance complex is believed to activate NMD (By similarity).

Belongs to the DNA2/NAM7 helicase family.

129.913 kDa

MSVDTYAPSSALSFLDMDDNELLPGADTQPTQYDYRDFTMPSTSQSQTQNDQLEIAQRCSAGDSHPRLASITNDLADLQFEEEDDEPGSSYVKELPPHACKYCGIHDPATVVMCNNCRKWFCNGRGSTSGSHIINHLVRAKHREVTLHGEGPLGETILECYSCGVRNVFVLGFIPAKADSVVVLLCRQPCAAQNSLKDMNWDQEQWKPLIADRCFLAWLVKQPSEQGQLRARQISAAQINKLEELWKENIEATFQDLEKPGIDSEPAHVLLRYEDGYQYEKTFGPLVRLEAEYDQKLKESATQENIEVRWDVGLNKKTIAYFTLAKTDSDMKLMHGDELRLHYVGELYNPWSEIGHVIKVPDNFGDDVGLELKSSTNAPVKCTSNFTVDFIWKCTSFDRMTRALCKFAIDRNSVSNFIYSRLLGHGRADSNDEVLFRGPQPKLFSAPHLPDLNRSQVYAVKHALQRPLSLIQGPPGTGKTVTSATIVYQLVKLHGGTVLVCAPSNTAVDQLTEKIHRTNLKVVRVCAKSREAIDSPVSFLALHNQIRNMETNSELKKLQQLKDETGELSSADEKRYRNLKRAAENQLLEAADVICCTCVGAGDGRLSRVKFTSILIDESMQSTEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAARAGLSQSLFERLVVLGIRPFRLEVQYRMHPELSQFPSNFFYEGSLQNGVCAEDRRLKLDFPWPQPERPMFFLVTQGQEEIAGSGTSFLNRTEAANVEKITTRFLKAGIKPEQIGIITPYEGQRAYLVQYMQYQGSLHSRLYQEIEIASVDAFQGREKDIIIMSCVRSNERQGIGFLNDPRRLNVALTRAKFGIIIVGNPKVLAKQQLWNHLLNFYKDRKVLVEGSLNNLKESLIHFQKPKKLVNSMNIGAHFMSTIIADAKEVMVPGSIYDRSGGYGQGRQMVGQSMNGGQYGGSGGGPYGNSPLGYGTPSSNSMVGFGLGNGGNGAAGGNNNFGGAGPSWAAAHLHHDSIGYISNEHGAAALGNMPVPVGMFMNMSNIPPRFYNQHQQAIMAVKQNRAIQQQTGNFSPGNSGPGVTGVGVGRSATPGGNKKTNKLGKSRVTGGGTGGAPLTQGSSVCNAAPYSQHPMPLSLQMTQPSGFALSQQPELSQDFGQISQMDGLLSQDVAFNASGERSLNQFSQPY

UPF1

Regulator of nonsense transcripts 1

1129 amino acids

RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability.

Belongs to the DNA2/NAM7 helicase family.

124.345 kDa

MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPGGAGAGAAAGQLDAQVGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPIHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSSMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Upf1

Regulator of nonsense transcripts 1

1124 amino acids

RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (By similarity). Essential for embryonic viability.

Belongs to the DNA2/NAM7 helicase family.

123.967 kDa

MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGAGGPGGAGAGGAAGQLDAQVGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAFFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVVIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLSYYKEQKALVEGPLNNLRESLMQFSKPRKLVNTVNPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQISMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKTKTGRGGRQKNRFGLPGPSQTTLPNSQASQDVASQPFSQGALTQGYVSMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

upf1

ATP-dependent helicase upf1

925 amino acids

Required for rapid turnover of mRNAs containing a premature translational termination codon.

Belongs to the DNA2/NAM7 helicase family.

104.529 kDa

MSLGLQPNNDISSLVSSKNMTSENGLEHQFEELLVEKQYSEEHCAYCHIKNPNSILKCLHCNKWFCNVRGKSGASHIISHLVRARHKQVALHSHSSLSDTVLECYNCGTRNVFLLGFIPAKAKTVVVLLCRQPCARASIAKDMNWDLTQWQPIISDRQFLPWLITPPSEEEQKLAIPITSQQMVRLEELWRKDPNANLEDLDKPIEDDSLPSVELRYKDAHAYQAVLSPLIQAEADYDKRLKESQTQKDVVVRWDQAINKRYTAWFLLPKLESGEIRLAIGDEMKLTYEGELRAPWSSTGYVIKIPNNVSDEVGLELKRSDKVPIECTHNFSVDYVWKSTSFDRMQTALRLFATDGSRLSSFLYHKLLGHDIPPSFLKPKLPSDLSVPNLPKLNASQSEAVRAVLSKPLSLIQGPPGTGKTVTSASVVYHLATMQSRKRKSHSPVLVCAPSNVAVDQLAEKIHRTGLRVVRVAAKSREDIESSVSFLSLHEQIKNYKFNPELQRLLKLRSENNELSIQDEKKLRILVAAAEKELLRAAHVICCTCVGAGDRRISKYKFRSVLIDEATQASEPECMIPLVLGAKQVVLVGDHQQLGPVVMNKKVALASLSQSLFERLIILGNSPFRLVVQYRMHPCLSEFPSNTFYEGTLQNGVTTSERIARHVDFPWIQPDSPLMFYANFGQEELSASGTSFLNRTEASTCEKIVTTFLRSNVLPEQIGIVTPYDGQRSYIVQYMQNNGSMQKDLYKAVEVASVDAFQGREKDFIILSCVRSSEHQGIGFVNDPRRLNVALTRAKYGVIVLGNPKVLAKHALWYHFVLHCKEKGYLVEGTLNSLQKFSLTLTPPQKPQKFKRDLNVQRSLSPIQNAGSAMLPSFSNLPNLYSSSYLEEWNVFAQYKRRESNATDFEDFRSQVGDDESKFDEPTRF