Protein
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase
Organism
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Function
Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).
Similarity
Belongs to the class II aldolase/RraA-like family.
Sequence
MEARTTDLSDLYPEGEALPMVFKSFGGRARFAGRVRTLRVFEDNALVRKVLEEEGAGQVLFVDGGGSLRTALLGGNLARLAWERGWAGVVVHGAVRDTEELRGVPIGLLALAATPKKSAKEGKGEVDVPLKVLGVEVLPGSFLLADEDGLLLLPEPPSGVRSGG