Protein
4-hydroxy-2-oxovalerate aldolase
Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Function
Catalyzes the retro-aldol cleavage of both 4-hydroxy-2-oxopentanoate (HOPA) and 4-hydroxy-2-oxohexanoate (HOHA) to pyruvate and acetaldehyde or propanaldehyde, respectively. The aldehydes produced by this reaction are directly channeled to the dehydrogenase TTHB247, ensuring that these toxic aldehydes are sequestered from cellular components. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds. Appears to be stereospecific since it can cleave (4S)-4-hydroxy-2-oxopentanoate but not the (4R) isomer. Is not able to catalyze the aldol addition of 2-oxobutyrate with acetaldehyde; this indicates that the enzyme is specific for pyruvate as the carbonyl donor.
Similarity
Belongs to the 4-hydroxy-2-oxovalerate aldolase family.
Sequence
MSWDLSTAKPPVVVDTTLRDGSHAHRHQYTVEEARAIAQALDEAGVYAIEVSHGDGLGGSSLQYGFSRTDEMELIRAVRETVRRAKVAALLLPGIGTRKELKEAVEAGIQMVRIATQCTEADISEQHFGMAKEMGLEAVGFLMMSHMRPPEFLAEQARLMEGYGADVVYIVDSAGAMLPEDAYARVKALKEALSRAKVGFHAHNNLGLAIGNTLAALAAGADWVDATLRGYGAGAGNAPLEVLAAVLDKAGLNPGLDVFKLLDAAEYVMGPILHFQPYPDRDSVAIGYAGVYSTFLLHAKRIGKELGVDPLAILLELGRRQAVAGQEDWILRVALELKEKEAGALAD