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ROMT-15

Gene
ROMT-15
Protein
Tricin synthase 1
Organism
Oryza sativa subsp. japonica
Length
252 amino acids
Function
Catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. No 3',4',5'-trimethylated ester derivatives are produced. Can use caffeoyl-CoA, 5-hydroxyferulic acid, luteolin, tricetin, quercetin, myrcetin and 7,8-dihydroxyflavone as substrates, but not naringenin, apigenin or kaempferol. The 2,3-double bond and the O-dihydroxyl group of the substrate are both required for catalytic activity of the enzyme.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family. CCoAMT subfamily.
Mass
27.772 kDa
Sequence
MTTGNGDAPVIKNAHSDIDSTNKTLLKSDALYKYVLDTTVLPREPECMRDLRLITDKHQWGFMQSSADEAQLLGMLLKMAGAKRTIEVGVFTGYSLLATALALPEDGKVVAIDPDRESYEIGRPFLEKAGVAHKVDFREGKGLEKLDELLAEEAAAGREAAFDFAFVDADKPNYVKYHEQLLQLVRVGGHIVYDNTLWAGTVALPPDTPLSDLDRRFSVAIRDLNSRLAADPRIDVCQLAIADGITICRRLV