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RNL

Gene
RNL
Protein
tRNA ligase 1
Organism
Arabidopsis thaliana
Length
1104 amino acids
Function
Essential component of stress-response pathways entailing repair of RNA breaks with 2',3'-cyclic phosphate and 5'-OH ends (PubMed:23515942). Tri-functional enzyme that repairs RNA breaks with 2',3'-cyclic-PO(4) and 5'-OH ends. The ligation activity requires three sequential enzymatic activities: opening of the 2'3'-cyclic phosphodiester bond of the 5' half-tRNA leaving a 2'-phosphomonoester (CPDase activity), phosphorylation of the 5' terminus of the 3' half-tRNA in the presence of ATP (kinase activity) and ligation of the two tRNA halves in an ATP-dependent reaction (ligase activity) (PubMed:24554441, PubMed:23515942). Deficient in transferring AMP to pRNA(OH) to form AppRNA(OH) but proficient at sealing pre-adenylylated AppRNA(OH) (PubMed:23515942). CPDase and kinase reactions are almost insensitive to RNA length, whereas the ligase activity decreases with shorter RNA size. Can also splice DNA ended by a single 3'-terminal ribonucleoside 2',3'-cyclic-PO(4) (PubMed:24554441). Binds to mRNA, mature and immature (PubMed:20844078). Exhibits tRNA ligase activity in vitro (PubMed:15653639, PubMed:24554441). Required for the splicing of precursor tRNA molecules containing introns (PubMed:16428247, PubMed:20844078). Can circularize an intron cleaved from a pre-tRNA by splicing endonuclease in vitro (PubMed:20844078). Seems not involved in unfolded protein response (UPR) in the endoplasmic reticulum (PubMed:20844078). Involved in auxin signaling and polar transport during organ morphogenesis (PubMed:25892242).
Similarity
Belongs to the TRL1 family.
Mass
123.205 kDa
Sequence
MDAPFESGDSSATVVAEAVNNQFGGLSLKESNTNAPVLPSQTTSNHRVQNLVWKPKSYGTVSGSSSATEVGKTSAVSQIGSSGDTKVGLNLSKIFGGNLLEKFSVDKSTYCHAQIRATFYPKFENEKTDQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGHKGGAYAKNSFGNIYTAVGVFVLSRMFREAWGTKAPKKEAEFNDFLEKNRMCISMELVTAVLGDHGQRPLDDYVVVTAVTELGNGKPQFYSTSEIISFCRKWRLPTNHVWLFSTRKSVTSFFAAFDALCEEGIATSVCRALDEVADISVPASKDHVKVQGEILEGLVARIVSSQSSRDMENVLRDHPPPPCDGANLDLGLSLREICAAHRSNEKQQMRALLRSVGPSFCPSDVEWFGDESHPKSADKSVITKFLQSQPADYSTSKLQEMVRLMKEKRLPAAFKCYHNFHRAEDISPDNLFYKLVVHVHSDSGFRRYHKEMRHMPSLWPLYRGFFVDINLFKSNKGRDLMALKSIDNASENDGRGEKDGLADDDANLMIKMKFLTYKLRTFLIRNGLSILFKDGAAAYKTYYLRQMKIWGTSDGKQKELCKMLDEWAAYIRRKCGNDQLSSSTYLSEAEPFLEQYAKRSPKNHILIGSAGNLVRTEDFLAIVDGDLDEEGDLVKKQGVTPATPEPAVKEAVQKDEGLIVFFPGIPGSAKSALCKELLNAPGGFGDDRPVHTLMGDLVKGKYWPKVADERRKKPQSIMLADKNAPNEDVWRQIEDMCRRTRASAVPIVADSEGTDTNPYSLDALAVFMFRVLQRVNHPGKLDKESSNAGYVLLMFYHLYEGKNRNEFESELIERFGSLIKMPLLKSDRTPLPDPVKSVLEEGIDLFNLHSRRHGRLESTKGTYAAEWTKWEKQLRDTLVANSEYLSSIQVPFESMVHQVREELKTIAKGDYKPPSSEKRKHGSIVFAAINLPATQVHSLLEKLAAANPTMRSFLEGKKKSIQEKLERSHVTLAHKRSHGVATVASYSQHLNREVPVELTELIYNDKMAALTAHVGSVDGETVVSKNEWPHVTLWTAEGVTAKEANTLPQLYLEGKASRLVIDPPVSISGPLEFF