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RAG1

Gene
rag1
Protein
V(D)J recombination-activating protein 1
Organism
Oncorhynchus mykiss
Length
1073 amino acids
Function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination (By similarity).
Similarity
Belongs to the RAG1 family.
Mass
121.045 kDa
Sequence
MEETYAPRCSMPAELHHPYSKFSDWKFKLFRVRSMERAPLPGEMQLERGALSGVVASAPLGETVGDVVGLPGSVMKLWLGGKSKENVEGPGKRVDLKLQEMDTYMNHLRCLCRLCGGALRKAKGPEHEVQGLLDEASMSALRRVGCKATSWPEVILKVFKVDVAGDMEVVHPPFFCQRCWTLAMRGGGFCSFSRTHVPGWRPHTTLCLLCTPRNPHYRGERKRRKPTRGAQHLAKRTKWDLQDNAAIVGEKRAWRTVIDPPQGPGLRPWVRSSVQRAQWVKSITLCQKEHLSARLLSEDLPVDFLSSVTCQVCDHLLSEPVQSPCRHLFCRSCIAKYIYSLGPHCPACTLPCGPADLTAPAKGFLGVLHSLPLLCPRESCGEQVRLDSFRAHCLGHHLEEVDGDHKSAENSLDNFLPVNKGGRPRQHLLSLTRRAQKHRLRDLKTQVKVFAEKEEGGDTKSVCLTLFLLALRAGNEHRQADELEAMMQGRGFGLHPAVCLAIRVNTFLSCSQYHKMYRTVKATSGRQIFQPLHTLRTAEKELLPGYHPFEWQPALKSVSTSCHVGIIDGLSGWIASVDDSPADTVTRRFRYDVALVSALKDLEEDIMEGLRERGLEDSACTSGFSVMIKESCDGMGDVSEKHGGGPPVPEKPVRFSFTIMSVSIQAEGEDEAITIFREPKPNSEMSCKPLSLMFVDESDHETLTGVLGPVVAERNAMKHSRLILSVGGLSRSFRFHFRGTGYDEKMVREMEGLEASGSTYICTLCDSTRAEASQNMTLHSVTRSHDENLERYELWRTNPHSESAEELRDRVKGVSAKPFMETQPTLDALHCDIGNATEFYKIFQDEIGEVYHKANPSREQRRSWRAALDKQLRKKMKLKPVMRMNGNYARKLMTREAVEAVCELVCSEERQEALRELMGLYIQMKPVWRSTCPAKECPDELCRYSFNSQRFAELLSTVFKYRYDGKITNYLHKTLAHVPEIVERDGSIGAWASEGNESGNKLFRRFRKMNARQSKTFELEDVLKHHWLYTSKYLQKFMEAHKDSAKALQATIDTVGSQETQEDADMSLDVPDF

Gene
rag1
Protein
V(D)J recombination-activating protein 1
Organism
Danio rerio
Length
1057 amino acids
Function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination (By similarity).
Similarity
Belongs to the RAG1 family.
Mass
121.247 kDa
Sequence
MEKGRWSSEDAPRASMPDELSHPKFSEWKFKLFRVRSMEKAPVQNETPVEKENQPELAMEKTSSQGSVMRLCFGGKSKENVESARGRVDLKLQEIDTHMNLLKNMCRLCGIAIQKAKGPSHEVQGVLEESSRCALRRMGCKLVTWPEVILKVFKVDVTTDMETVHPSLFCHRCWTAAIRGGGFCSFTNTRIPDWKPHTSQCNLCFPKKSSFQRVGRKRTKPLKSAHILPKRFRRDSSESSRVWRQTTENPDGKEWLKLSVQRGQWVKNITRCQRDHLSTKLIPTEVPADLIRAVTCQVCDHLLSDPVQSPCRHLFCRLCIIRYTHALGPNCPTCNQHLNPSHLIKPAKFFLATLSSLPLLCPSEECSDWVRLDSFREHCLNHYREKESQEEQTPSEQNLDGYLPVNKGGRPRQHLLSLTRRAQKHRLRDLKNQVKTFAEKEEGGDVKSVCLTLFLLALRAGNEHKQADELEAMMQGRGFGLHPAVCLAIRVNTFLSCSQYHKMYRTVKATSGRQIFQPLHTLRNAEKELLPGFHQFEWQPALKNVSTSWDVGIIDGLSGWTVSVDDVPADTISRRFRYDVALVSALKDLEEDIMEGLRERALDDSMCTSGFTVVVKESCDGMGDVSEKHGSGPAVPEKAVRFSFTIMSISIRLEGEDDGITIFQEQKPNSELSCRPLCLMFVDESDHETLTAILGPVVAERKAMMESRLIISVGGLLRSFRFFFRGTGYDEKMVREMEGLEASGSTYICTLCDSTRAEASQNMVLHSITRSHDENLERYEIWRKNPFSESADELRDRVKGVSAKPFMETQPTLDALHCDIGNATEFYKIFQDEIGEVYQKPNPSREERRRWRSTLDKQLRKKMKLKPVMRMNGNYARRLMTREAVEAVCELVPSEERREALLKLMDLYLQMKPVWRSTCPSRDCPDQLCQYSYNSQQFADLLSSMFKYRYDGKITNYLHKTLAHVPEIVERDGSIGAWASEGNESGNKLFRRFRKMNARQSKTFELEDILKHHWLYTSKYLQKFMEAHKNSVKAMQATFNPEETPEEADNSLDVPDF

Gene
rag1
Protein
V(D)J recombination-activating protein 1
Organism
Xenopus laevis
Length
1045 amino acids
Function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination (By similarity).
Similarity
Belongs to the RAG1 family.
Mass
119.893 kDa
Sequence
MEVALPNVPTKMQHPFTKYSEWKFKLFRVKSLERRPSEEETEGSEVSYNSSQETYPKSTVVLEDLSLGSAPQSPTNFKPQQSEKSNVVDNHETDLKRLEEEAHITVIQQLCRICGASFKMDQQNRSYPVHGPVDSETHDILRRREKKVTSWPELISKVFKTDVRADVDTIHPTQFCHNCWTIMNQKFSNISSEVYFPHNQAVEWTPHSANCYVCHSSKPWGKRKSAPQLNPHKMKKRKRGPEFVKKSKTSSGNSIQWKNMKAFNQMKDSCKKIHLDNNLLVLDYPSDFVKSVSCLVCEHILSDPVQTSCKHLFCRICILKYIKLMGCYCPSCKYPCFPTDLTVPVKSYLNVLNALLLKCTVSGCDEEISLGKYSHHISKHKETKGKEVYAHINKGGRPRQHLLTLTRRAQKHRLRELKMQVKAFADKEEEGDVKSVCLTLFLLALRARNEHRQADELEAIMEGRGAGLHPAVCLAIRVNTFLSCSQYHKMYRTVKATTGRQIFQPLHALRNAEKALIPGYHTFEWRPPLKNVSTRTDVGIIDGLSGLNQSLDEYPVDTISKRFRYDAALVSALKDMEEDILEGLKAQDLDDYVSGPFTVVVKESCDGMGDVSEKHGSGPPVPEKAVRFSFTVMNISVPNKNGPVRIFEETKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKTAELLLEMGGILRNFKFSFRGTGYDEKLVREVEGLEASGSLYICTLCDATRLEAAQNLVNHSITRSHCENLQRYEMWRSNPHHESPDELRDRVKGVSAKPFIETLPSIDALHCDIGNAAEFYRIFQLEIGELYKNLSATKEEKKRWQATLDNHIRKRMNLKPIMRMNGNFARKLMSKETVEAVCELVPCEERQAALTELMDLYLKMKPVWRSSCPAKECPELLCQYSFHSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKFYEMEDVLKHHWLYTSKYLQKFMNAHNNLKNQGFTVDLDNPDLEQRLESSMESLESMEF

Gene
RAG1
Protein
V(D)J recombination-activating protein 1
Organism
Homo sapiens
Length
1043 amino acids
Function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1 (By similarity).
Similarity
Belongs to the RAG1 family.
Mass
119.097 kDa
Sequence
MAASFPPTLGLSSAPDEIQHPHIKFSEWKFKLFRVRSFEKTPEEAQKEKKDSFEGKPSLEQSPAVLDKADGQKPVPTQPLLKAHPKFSKKFHDNEKARGKAIHQANLRHLCRICGNSFRADEHNRRYPVHGPVDGKTLGLLRKKEKRATSWPDLIAKVFRIDVKADVDSIHPTEFCHNCWSIMHRKFSSAPCEVYFPRNVTMEWHPHTPSCDICNTARRGLKRKSLQPNLQLSKKLKTVLDQARQARQHKRRAQARISSKDVMKKIANCSKIHLSTKLLAVDFPEHFVKSISCQICEHILADPVETNCKHVFCRVCILRCLKVMGSYCPSCRYPCFPTDLESPVKSFLSVLNSLMVKCPAKECNEEVSLEKYNHHISSHKESKEIFVHINKGGRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCMTLFLLALRARNEHRQADELEAIMQGKGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHHFEWQPPLKNVSSSTDVGIIDGLSGLSSSVDDYPVDTIAKRFRYDSALVSALMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPVVPEKAVRFSFTIMKITIAHSSQNVKVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSELMLELGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHAENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKNPNASKEERKRWQATLDKHLRKKMNLKPIMRMNGNFARKLMTKETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNALKTSGFTMNPQASLGDPLGIEDSLESQDSMEF

Gene
RAG1
Protein
V(D)J recombination-activating protein 1
Organism
Sus scrofa
Length
1043 amino acids
Function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1 (By similarity).
Similarity
Belongs to the RAG1 family.
Mass
119.068 kDa
Sequence
MAVSLPPTLGLSSAPDEIQHPHIKFSEWKFKLFRVRSFEKAPEKAQTEKQDSSEGKPSLEQSPAVLDKPGGQKSALPQPAFKPHPKFLKESHEDGKARDKAIHQANLRRLCRICGNSFNTTGHKRRYPVHGPVDGKTQVLLRKKEKRATSWPDLIAKVFRIDVKADVDSIHPTEFCHNCWSFMHRKFSSTPCEVYSPRNAAMEWHPHTLNCDICHIARRGLKRKSQQPNMQLSKKLKTVIDRARQARQRKRRAQARISSKELMKKIANCGQIHLSPKLLAVDFPAHFVKSISCQICEHILADPVETSCKHVFCRICILRCLKVMGSSCPSCHYPCFPTDLESPVKSFLSILNTLMVKCPAKECNEEISLEKYNHHISSHKESKETFVHINKGGRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCLTLFLLVLRARNEHRQADELEAIMRGQGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHPFEWQPPLKNVSSSTDVGIIDGLSGLSSSVDDYPVDTIAKRFRYDSALVSALMDMEEDILEGMRAQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPVVPEKAVRFSFTVMKITIAHGSQNVKVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSQLMLEMGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHAENLERYEVWRSNPYHETVDELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEAYKNPHASKEERKRWQATLDKHLRKKMNLKPIMRMNGNFARKLMTKETVEAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKYYEMEDVLKHHWLYTSKYLQKFMNAHKAFKNSGFTINLQRSSGDTLDLENSPESQDLMEF

Gene
RAG1
Protein
V(D)J recombination-activating protein 1
Organism
Oryctolagus cuniculus
Length
1042 amino acids
Function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1 (By similarity).
Similarity
Belongs to the RAG1 family.
Mass
119.007 kDa
Sequence
MAVSCTPTLGLSSAPDEIQHPHIKFSEWKFKLFRVRSFEKTTEENQKEKNSSEGKPSLEQSPAVLDKASGQKPEVAPPAFKVHPKFSKKFHDDGKARDKAIHQANLRHLCRICGNSLKTDEHNRRYPVHGPVDSKTQGLLRKKEKKATSWPDLIAKVFRIDVKTDVDSIHPTEFCHNCWRIMHRKFSGAPCEVYFPRNATMEWHPHAPSCDICYTARRGLKRRNHQPNVQLSKKLKTVLDQARQARQRKRRVQARITSKEVMKMIANCSKIHLSTKLLAVDFPAHFVKSISCQICEHILADPVETSCKHVFCRICILRCLKVMGSYCPSCQYPCFPTDLESPVKSFLCILNSLIVKCSAPECNEEVSLEKYNHHVSSHKESRDTFVHINKGGRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCLTLFLLALRARNEHRQADELEAIMQGRGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHPFEWQPPLKNVSSSTDVGIIDGLSGLSSSVDDYPVDTIAKRFRYDSALVSALMDMEEDILEGMRSQDLEDYLNGPFTVVVKESCDGMGDVTEKHGSGPAVPEKAVRFSFTVMKITIAHGSQNVKVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSELMLEMGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHAENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKNPSASKEERKRWQATLDKHLRKKMNLKPIMRMNGNFARKLMTIETVEAVCELIPSKERHEALRELMELYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNAVKNSGFTMNSQVSLEDPLGIEDSLESQYSMEF

Gene
RAG1
Protein
V(D)J recombination-activating protein 1
Organism
Gallus gallus
Length
1041 amino acids
Function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination (By similarity).
Similarity
Belongs to the RAG1 family.
Mass
119.917 kDa
Sequence
MSITSRMDLPEEIQHPYAKFSEWKFKLFKVRPFQKEPSDKSQCINKDQEQEVASTDKNITLHKDEEVPRGEKLILTQKDFMGNTQALEKDVNDMKIQDNTAHQNNLKQLCRICGVSFKTDCYKRTHPVHGPVDDETLWLLRKKEKKATSWPDLIAKVFKIDVRGDVDTIHPTRFCHNCWSIIHRKFSNTPCEVYFPRNSTMEWQPHSANCEVCHTPSRGVKRKSQPPNVQHGKRVKIIAERARVNRGIKNQVIKNKNVMKEITNCKNRHLSTKLLAVDYPADFIKSISCQICEHILADPVETTCRHLFCRTCILSCIRVMGCYCPSCWYPCFPTDLVTPVKSFLNILDSLSIRCPVPECDEEILHGKYGQHFSNHKEMKDKELYNPINKGGRPRQHLLSLTRRAQKHRLRELKRQVKAFAEKEEGGDIKAVCMTLFLLALRAKNEHKQADELEAIMQGRGSGLHPAVCLAIRINTFLSCSQYHKMYRTVKAVTGRQIFQPLHALRTAEKALLPGYHPFEWKPPLKNVSTNTEVGIIDGLSGLPLSIDDYPIDTIAKRFRYDTALVSALKDMEEEILEGMKAKNLDDYLNGPFTVVVKECCDGMGDVSEKHGSGPAVPEKAVRFSFTVMNIAIDHENERIRIFEEVKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKNSELLLEIGGILRTFKFIFRGTGYDEKLVREVEGLEASGSTYICTLCDATRLEASQNLVFHSITRSHAENLERYEIWRSNPYHESVDELRDRVKGVSAKPFIETVPSIDALHCDIGNATEFYRIFQMEIGEVYKNPDATKEERKRWQLTLDKHLRKKMKLKPMMRMSGNFARKLMSKETVEAVCELIKCEERHEALKELMDLYLKMKPVWRSSCPAKECPELLCQYSYNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKFYEMEDVLKHHWLYTSKYLQKFMNAHKTLRSQGFAIDPDDGLGDSLPPEISLESNDSVEL

Gene
Rag1
Protein
V(D)J recombination-activating protein 1
Organism
Mus musculus
Length
1040 amino acids
Function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1.
Similarity
Belongs to the RAG1 family.
Mass
119.185 kDa
Sequence
MAASLPSTLSFSSAPDEIQHPQIKFSEWKFKLFRVRSFEKAPEEAQKEKDSSEGKPYLEQSPVVPEKPGGQNSILTQRALKLHPKFSKKFHADGKSSDKAVHQARLRHFCRICGNRFKSDGHSRRYPVHGPVDAKTQSLFRKKEKRVTSWPDLIARIFRIDVKADVDSIHPTEFCHDCWSIMHRKFSSSHSQVYFPRKVTVEWHPHTPSCDICFTAHRGLKRKRHQPNVQLSKKLKTVLNHARRDRRKRTQARVSSKEVLKKISNCSKIHLSTKLLAVDFPAHFVKSISCQICEHILADPVETSCKHLFCRICILRCLKVMGSYCPSCRYPCFPTDLESPVKSFLNILNSLMVKCPAQDCNEEVSLEKYNHHVSSHKESKETLVHINKGGRPRQHLLSLTRRAQKHRLRELKIQVKEFADKEEGGDVKAVCLTLFLLALRARNEHRQADELEAIMQGRGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHPFEWQPPLKNVSSRTDVGIIDGLSGLASSVDEYPVDTIAKRFRYDSALVSALMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPAVPEKAVRFSFTVMRITIEHGSQNVKVFEEPKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSELTLEMGGIPRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDTTRLEASQNLVFHSITRSHAENLQRYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKHPNASKEERKRWQATLDKHLRKRMNLKPIMRMNGNFARKLMTQETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNALKSSGFTMNSKETLGDPLGIEDSLESQDSMEF

Gene
RAG1
Protein
Low-affinity glucose transporter
Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Length
567 amino acids
Function
Low-affinity glucose transporter.
Similarity
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.
Mass
63.124 kDa
Sequence
MSNQMTDSTSAGSGTEHSVDTNTALKAGSPNDLKVSHEEDLNDLEKTAEETLQQKPAKEYIFVSLCCVMVAFGGFVFGWDTGTISGFVNQTDFLRRFGQEKADGSHYLSNVRTGLIVSIFNIGCAVGGIVLSNIGDRWGRRIGLITVIIIYVIGIIIQIASVDKWYQYFIGRIISGLGVGGITVLSPMLISETAPKHLRGTLVSCYQLMITFGIFLGYCTNYGTKNYSNSVQWRVPLGLCFAWAIFMVLGMMFVPESARFLVETDQIEEARKSLAKTNKVSIDDPVVKYELLKIQSSIELEKAAGNASWGELITGKPSMFRRTLMGIMIQSLQQLTGDNYFFYYGTTIFQSVGMDDSFETSIVLGIVNFASTFFALYTVDHFGRRNCLLYGCVGMVACYVVYASVGVTRLWPDGPDHPDISSKGAGNCMIVFACFYIFCFATTWAPIAYVVISESYPLRVKGKAMAIASASNWIWGFLIGFFTPFITSAIHFYYGYVFMGCMVFAFFYVYFFVPETKGLTLEEVNEMYSEGVLPWKSSSWVPSSRRGAEYDVDALQHDDKPWYKAML

Gene
RAG1
Protein
Seed allergenic protein RAG1
Organism
Oryza sativa subsp. japonica
Length
163 amino acids
Function
Seed storage protein.
Similarity
Belongs to the cereal trypsin/alpha-amylase inhibitor family.
Mass
17.568 kDa
Sequence
MASNKVVFSVLLLVVLSVLAAAMATMADHHQVYSPGEQCRPGISYPTYSLPQCRTLVRRQCVGRGAASAADEQVWQDCCRQLAAVDDGWCRCGALDHMLSGIYRELGATEAGHPMAEVFPGCRRGDLERAAASLPAFCNVDIPNGPGGVCYWLGYPRTPRTGH

Gene
rag1
Protein
V(D)J recombination-activating protein 1
Organism
Ginglymostoma cirratum
Length
106 amino acids
Function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination (By similarity).
Similarity
Belongs to the RAG1 family.
Mass
12.598 kDa
Fragment
single
Sequence
RSTFPTRECPELLCQYSCNSQRFAELLRTEFKHRYEGKITNYLHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKSYELEGILKHHWLYTSKYL