Organism
Pseudomonas syringae pv. syringae (strain B728a)
Function
Arylacetonitrilase which is capable of hydrolyzing indole-3-acetonitrile (IAN) to the plant hormone indole-3-acetate (IAA), and allows the plant pathogenic bacterium to use IAN as a sole nitrogen source. Is also able to hydrolyze phenylpropionitrile (PPN), allowing the use of this compound as a sole nitrogen source. This enzyme may represent an additional mechanism for IAA biosynthesis or may be used to degrade and assimilate aldoximes and nitriles produced during host plant secondary metabolism.
Similarity
Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.
Sequence
MKEPLKVACVQAAPVFLDLDATVDKTITLMEQAAAAGAGLIAFPETWIPGYPWFLWLDAPAWNMPLVQRYHQQSLVLDSVQARRISDAARHLGLYVVLGYSERNKASLYIGQWIIDDHGETVGVRRKLKATHVERTMFGEGDGASLRTFETPVGVLGALCCWEHLQPLSKYAMYAQNEQIHVAAWPSFSLYRNATSALGPEVNTAASRVYAAEGQCFVLAPCAIVSPEMIEMLCDSDAKRSLLQAGGGHARIFGPDGSDLATPLGEHEEGLLYATLDPAALTLAKVAADPAGHYSRPDVTRLMFNPNPTPCVVDLPDLPISSESIELLRPDIALEV