Protein
Alanine--tRNA ligase, chloroplastic/mitochondrial
Organism
Populus trichocarpa
Function
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Similarity
Belongs to the class-II aminoacyl-tRNA synthetase family.
Sequence
MGGLKLPPQTLHGIHGGRRPLTAPSSKPSFTIQPNYSSSISTGFTTRNVALFSPSIFPCGYFILGGARERRFGARNTQASVQPVTEELVEDKTKENPVSGDAIRRRFLEFYASRSHKVLPSASLVPDDPTVLLTIAGMLQFKPIFLGKAPRQVPRATTAQKCIRTNDVENVGRTTRHHTFFEMLGNFSFGDYFKKEAIKWAWELSTKEFGLPADRLWVSVYEDDDEAFEIWHDEVGVPVERIKRMGEEDNFWTSGATGPCGPCSELYYDFHPERGYKNTDLGDDSRFIEFYNLVFMQYNKMDDGSLEPLKQKNIDTGLGLERLARILQKVPNNYETDLIYPIIEKAAELANISYALADDRTKMNLKIIGDHLRAIVYLISDGVLPSNIGRGYVVRRLIRRAVRTGRLLGVKGGGEDGVFLPAIAEKVIELSPHIDPDVKARGHSILDELQREELRFVQTLERGEKLLDQMLAEALLNAQKSETLPCLSGKDVFLLYDTFGFPVEITTEVAEEQGVKIDMDGFEVEMENQRRQSQAAHNVVKLAVENGGDLAENVHDTEFLGYDTLSARAVVESLLLNGKSVIQVSEGSEVEVLLNKTPFYAESGGQIGDHGFLYVTQDQSKQTAVVEIKDVQKSLGSVFVHKGTIREGVLEVGREVEAAVDAKLRQRAKVHHTATHLLQSALKKVIGQETSQAGSLVAFDRLRFDFNFHRPLHDSELEEIENLINGWIGDGTLLQTKVMSLTDAKEAGAIAMFGEKYGEQVRVVEVPGVSMELCGGTHVSNTSEIRAFKIISEQGIASGIRRIEAVAGEAFIEYINARDSQMKLLCSTLKVKAEEVTTRVDNLLEELRTVRNEVSALRAKAAVYKASMIASKAFSVGTSKTIRVLVESMDDFDADALKSAAEYLMDTLQDPAAIILGSCPDEGKVSLVAAFTPGVVDIGIQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLTNALEKARTDLILILTEKAN