PKS-NRPS1

Trichosetin synthetase PKS-NRPS1

3914 amino acids

Hybrid PKS-NRPS synthetase; part of the gene cluster that mediates the biosynthesis of trichosetin, a trans-fused decalin-containing tetramic acid with antimicrobial activity (PubMed:28379186). The PKS module of PKS-NRPS1 together with the enoylreductase (ER) catalyze the formation of the polyketide unit which is then conjugated to L-serine by the condensation domain of the PKS-NRPS1 NRPS module (By similarity). Activity of the Dieckmann cyclase domain (RED) results in release of the intermediate N-desmethylequisetin also called trichosetin (By similarity). Diels-Alderase (DA) is involved in endo-selective Diels-Alder cycloaddition to form the decalin ring (By similarity). The cluster does not contain the equisetin N-methyltransferase and consequently, trichosetin is isolated as final product (PubMed:28379186).

Belongs to the NRP synthase family.

431.704 kDa

MSDNEPIAIIGSACRFPGDSSSPSKLWDLLKSPRDLLTKVPPNRYNADAFYHADSKHHGTTNVRHSYFLNEDPARFDNNFFNIQPGEAEAIDPQQRLLMEVVYQGLCASGQTIEGLRGSPTAVYVGVMCDDWSGIITRDLEVFPQYGATGMARSIMSNRISYFFDWHGPSMTIDTACSSSLVAVHQAIQTLRSGESEVAIAAGANLILTPGRSKMWDQDVNGYARGEGIAAVVLKPLSAAIRDNDHIDCIIRATGVNQDGRTPGLTMPSATAQADLIRSTYARAGLDINKPEDRPQFFHAHGTGTPAGDPREAEAIYRAFYSDVKDDKLYVGSIKTVLGHTEGTAGLASLIGTALAIQNKTIPPNMHFDVLNPKIKPFYDNLEVPTKAIAWPETHKGQPRRASINSFGFGGTNAHAIIEAYEPATTDSAPGPLFSPLTFSASSEPSLRSLLSSYSDHLKSNPDLSLKDLAYTLQTRRSTLAYRVAITASDVEDAYTQLDTIGNGEQSSTIGVRQVTKASPKIMGVLTGQGAQWPRMGARLVEESAFASQRLFELDEALSSLPKDDRPSWTLREMILADSKSSRIAEAAISQPLCTAVQVVLVDLLRQAGVELSSVVGHSSGEIGAAYAAGLLTARDAIRVAYYRGLYAKLAQSPNGRKGAMMAVGTTFDDASEFCELDAFQGRIQVAARNSSSSITLSGDEDAIVEAIETFKDEGKFARQLKVDTAYHSAHVLPCAKPYLDAMERCQIESANPTSTKWYSSVHDGQAMTAELLTPQYWVDNMTSAVLFSPAVEHAWREGGPYDVIIEVGPHPVLKTPCLDTLEDMTGDRPPYSGVLGREKDDIQQFASGLGFIWTQLGAGSATFERFEKVASDSKSIPSFIHDLPNYPFDHARQFMSMSRVSGWYNSMQEAPHPILGRRCHDRETSQTIQWRNVLNPKEIPWLHGHQIQGQIIFPATGYISMAIEAVNIIAGSDLGLVTIEDLRIGRALAFSDDDASVESMFDLRIISRSEKEIEAEFSCYSGLPQNHTTSMVLNATAHVKASLSVPTAQKLPNLKIDDFNLRKVEVDRFYDFLGRLGYNYSWPFHGTTSIRRKANYATGTLEDQSGSEWEDQLLVHPGMLDTSLQTTFAAFCCPGDERMWALHLPTSFRSIVVNPYFTSAGIGKQKSFQYQSVAIQERKASKVIVELNLLSEETGDTFLQIEGMELVPFSPATPENDAVLFSRFDYRLASPDGELTAAEYSFRDEDYKMALDSERIAFYYLRRLVETITPEDKANTLPHYRHLVEWAAHVVPQVIDGRNPHIPSSAQKDTHEDIQNILKKHYERVDVRLLESVGENLPQVIRENGNILEHMTKDGMLDDVYEEGFGLDLVNKYIAHMTAQIAHRYPRMNILEIGAGTGGSTREILPRLGSAFSTYTYTDVSGGFFDTAQDRFKDYAERMIFKTFDMNISPGSQGFTEGTYDLVIASNVLHATLELEDMMKHVRSFLKPGGFLIILETVNNDCLRVGLPMGSLPGWWLGAEHGRRWGPTLTLPQWDSLLWKCGFGGIDTTTPPVHKILPGHVFCAQALDDRVEILRSPLSHLSDLPETKSTELVIVGGETLKVHRMCEQISRRLKPKYASIARFNSIEELNTSGLADSCAVVSLTELDEPLFANMTSDKLDALKTLWKQGGSILWVTSGARDENPYSYMTTGVGRCMRFEYPNITLQALDIKAMTDRTPGLVAEHLLRLELLDKWSKELRPEELLWSLEPEIYVDDDTTIVPRLYPYESGNARYNAERRNIVEDADLQTDRVIFVENEGKWEVQHASPLHIPQELPFASKMKTIRITHFSPATINFAPGVSLMVCAGIDAASGERLLAVTHVAESPISVPADWCIPLGELDGVDTLANVSAFLIASSILKHVATEETLVVHGAPSRLASALEGLAKESSITVFLTTSERANKNGWQYIDSHLPERVIKASIPRSATKFINLSQDANMGETGRAISACLPRYCEVINTERLFIWGKLIRESVSKEDISIVFNRAFYETKSLSSTATDARLISLQDVSGVSAAEVRFAVVDCIDSSVKASIRPIDDGRIFQADKTFLLIGLSGELGQSLGKWMVEQGARNIVLTSRRPNVSQHFLDEMATMGATVKALPMDVTNRDSLHACVDTIQKTLPPIAGVVNGAMVLRDALFENMPYEDFMKVLSPKVLGSQLLDELFYDTPLDFFIFFSSTTAVMGNSGQSNYIAGNMFMNALAAQRKKRGVAASSIDISSIIGLGYVERAEDLSEDTFIKMGYKPMSEQDLQKLFAEAIVLGRPECDEVCELVTGVTPIYTDAQASDQYLKDVKFGHFLMERLDTQAYTGKTSTVPVRVQLADVKTKADAVAIIKGMFSLLTPVIIADLERFLHFEQGVDSLMAVEVRSWFIKELDIDIPVLKILGGMSVPDLIEESLNLISDSILDVSSLEAGSTPTTQPPKPTLQIARVTPPESSHGTSDDSKQQTGSDSSRSPIDTPLTSMEIQEPAKAEDSTDNSTPLKTFPNELSSIMSYGQAGFWFLNDYLVNKRAFNMAVMLKLTGQVRVQALEKAVNLVAERHEVLRTRFFWGDDGDERTPLQGINPADLKLTTKKVADESEAGMELKKLHDEEWDLSRGEGVKITLLSLSDNVHFLLLGMHHIYIDGYSFSVFFKDLEVAYTKNTLPSLPVESQYRSFALQQRQMYANGNLTKSIEYYRRSFPKEFSPIQLFPFALTPARQFANDYSQHEAKMSIDPELAPKVRQLARVNRSTSFHVYLAALELLLFTLLPNIEEVFIGIADANRGDKKFMGSLGFFLNLLPLRFRRKRRGTQLSSIIQTARDTAYGALQHSQLPFDVLLRELNVPRSDKYTPIFQVFMDYRQVVQERSSWGGCKLHGEKWHNAGTGYDIALEVNENITTDTLLGLRLQKQLYSEEHTALLLRSYLSVLEYMVQGTNKAADTVPPWSKDDIQVALDAGKAPEFQPKWQSTISHHIDQTIQANSTKVALKDGNGTVLTYEQMGNRVNSITQALIDAGTTQGTVVGVFQEPSSDWICSLLAIFKAGAVYVPLDLRNSIPRLASIVKASRPSLIITDHTTDDKVELIGAKYITQLQLSKVVDQEFKEPNRAKVGSLAVILFTSGSTGEPKGLMMTHTNLMSYAEVSSKTFSKPDESLMVLQQSPFSFDFSLDQTVAALANGGCLCIVPASKRGDPDEISKIMVKEGVTYTTATPSEYDLWLRYSTSTLRQCTSWKYAFSGGEAMSHKLAREFGTLSLKNLHVFNGYGPAETTILSHRIDLQYTDPDLPDPLPAGCPMPGFSVCIVDEKMRPVPLGVQGEIVLGGPCIVSGYLSMPDATKDKFLPDTFFGTSGKVYRSGDRGRLCHDGLLFCDGRLEDSNMIKLRGFRVELDEVEKTIISHSAGTLSHAVVTLRGTEEGRYLAAHVVFAPEFPEQNRESIMKTLRQTLPLPPYMRPSVFQILADIPRTAHLKVDRKAIQEMPVQISASHDSGTLTVAEQRLSELWRRVLPLDPGSLSPESDFFLIGGNSILLVKLQALLRQTFKTAPKLVALMGASTLGTMAVVLESCGSVGVIDWDQETALPNGLQGAVALRPVNKITDITVLLTGSAGYLGRHLVPALVEDPRVTRVHCLVRSVNNEKLSTSSSSKVRVIESDLSKPGLGLSASTYSRLAEETDVIIHSAANRSFWDRYEVLKPDNLDSVKELVRFAASSGRSIPLHFLSSGAVKIYDGDVVTPPTDGSDGYVATKWASETFLRNAAGSIGLPVYAHRPTVSSKAQTKTDQASIVDELFSIVKFLGVRPSFEGVTGSVDVLPTGDIVKAIQDSVLSSSAGGEGYNVLQHEAHQRAFVEDFAGVVRADDSLNKLPSISILDWFGKAKKAGFSYFLASQNLVMGEGEGHLVSRR