Protein
ATP-dependent DNA helicase PIF2
Organism
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Function
DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of mitochondrial (kinetoplast) genome stability. Essential for replication of kinetoplast maxicircles, but not minicircles.
Similarity
Belongs to the helicase family. PIF1 subfamily.
Sequence
MSSKTVVWSSLGRFMRTSHHRLLDEKLFPASHNRHVQSRLLSTPLSLIAVPSWMSKCRTSAVRLSRHPPQVSGQEPCAVTASSSVGTWHGSSPAGLRRCQHVRSLNGWTTPGDVPIRHGSRSIATIATGCGGVGAGGINSSVDVAEVSGVSTSGVAAAPTVALPPGPPVEKPPLICVVRRATRGGAAADSVGTGTPGKCDKPPDSSNTLVGTIARVLSSSGECGRHSNANQEGYSLGSRPARKILVKLEADTVAAPLADTKEKQIYVQQQPVVVAQSPLARSSSTVTWEEGDTLVYNIFTQRVVKSSSASIRALSVLGVGVRSSEKELSVVDPHALVEFRDKLREKEVSWPSAWRSSLFNQLQHVLLKDQPMEEMISRVHELLQLHYQRVKRSCVANVTKGEPDTKTEEANPAGDEVAINGEMTYHERLLGYPDLNEEQRRVVDFVLRGYNTYIGGGAGTGKSLLLRVIRQELVSRGLTVATTATTGIAARRLNGATLHHCFGVNVYGEFTRRAELKEFDVIIIDEVSMLSKELFESLEFQLRRANGVDLPFGGVQVILSGDFLQLGAICSVSLVHSSVFRRNFAMLKLQRVVRQEGSSIFAQQLQELRRGTVPHDLQDTVQFLSPPETAKWLEGEGKGAVKLLPTNKEVDEVNQAELDKLPSDLVVYPAQMQAPSLVGRWTATYILEAVVKDTKMIDTHKLTRALEQYVLDFLQKTPYASDYTLPVVGQRYIVLYKLFVDAFAFRVRIPQDMSEKDMRDLALHLRGLETWLPACGLGVFLREILDSPDGLHTDADDYTLTRYAELHPMASPLRLKKGAKVMLRTNLAPGLVNGSLGVVVGFKELSAKHLPRFVNTPGRIAAVENYAEYLRYEHGFTTAFAPEVDFGGGRVIVVPPVLFSVGGLSNTNHYHVGIVSLPLSLAYAFTVHKVQGLTLAGRVHLELSRMWPCDHLLYVAMSRVRNPEQLTVSSFHNSLVRCASECLLFDDSLPPVEQVRVLPHFFQASWQRTPSRRKAALQRKREQAKQSKQKKAAKLKEAMIKQAKEATP