Protein
Beta-mannosidase B
Organism
Thielavia heterothallica
Function
Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of beta-mannosidic oligosaccharides of various complexity and length. Prefers mannobiose over mannotriose. Is also severely restricted by galactosyl substitutions at the +1 subsite (By similarity). Has no activity against polymeric mannan.
Similarity
Belongs to the glycosyl hydrolase 2 family. Beta-mannosidase B subfamily.
Sequence
MAPRVVIPLDQNWEFRQADKPDSKFLPVSQFPTNVHLDLQHHGLIPDPFIGKNELLVQWVGEAQWTYRTVFAAPPVPEGARAVIAFDGLDTFATVVLNGTTILESDNMFLPHRVEVTSVLKAEGNELVITFDSAYLRGCKLVEQHPNHKWGCWNGDVSRLAVRKAQYHWGWDWGPTLLTCGPWRPVHLEIYESRLSDLYAETVVDKSLKRASVKVTAVAERRADRVRFDIALDGQQVATETAELDATSGEATVSFLIDSPALWYPVRYGKQPLYDIRATLLAGDDEVDTLSKRIGLRRAELIQRPLEGQPGTSFFFEVNNIRIYCGGSDWIPADNFIPRISRRRYYDWVRLVAEGNQFMIRVWGGGIYEEQAFYDACDELGILVWQDFMFGCGNYPAWPALLESIRREATENVKRLRHHPSIVIWAGNNEDYQYQESEGLTYDYANKDAESWLKTDFPARYIYEKILADVCADLVPSTPYHPGSPWGAGLNTHDATVGDIHQWNVWHGTQEKWQNFDRLVGRFVSEFGMQAFPAVKTIDAYLPLGRDDPDRYPQSSTVDFHNKAEGHERRIALYLVENLRYAPDPLEHFVYCTQLMQGECLASAYRLWKREWRGPGREYCGGALVWQTNDCWPVTSWSIVDYYLRPKLAYFTVKREMAPVSIGITRRTHLHPRDRHTRVNVDVKTQIEVWASNLTLEDLTVDCVLKAWDVESGEETFSETVAAALLLRENRSTEIAALDVPVRQKNVGEEGRIVVAAYLVDKEGRQMARYVNWPEPLKYVHLQKPRALRAQLTADYSAVEVSAEVPVKGVALECEDDGVRFDDNLVDIVPGEVVTIGVSGAGKDTKIETRYLGMI