Function
Non-ribosomal peptide synthetase, able to activate proline and AEO (2-amino-9,10-epoxi-8-oxodecanoic acid), and epimerize L-Pro. Catalyzes the production of HC-toxin: a cyclic tetrapeptide. Activates and thioesterifies L-Pro, and epimerizes it to D-Pro; also uses D-Ala as a substrate but this is epimerized from L-Ala by TOXG.
Sequence
MTMPHHSSGPAKDSPLCRFPPFPGGNPVFTNIRREKVNFQLPPPDHALLAAAWAVLLRLYTGHVKTCFESATSDQEANLVTYEARDSDTLQTIVLRGACVSSTAEEKAGLRDLNTAVVRTTVSIDSWTDEMLQDKIAALLQPGKEIVLFQTPSGCVLVYMQSFMSAMEVKNVSSTLTYIMSSDPDKTAIRNLSISPRDLAQIMRWNDRKLKSERTNLVYDLFSARAHEQDANMAIDAWDGRMSYTELERVSSTWARQLQKQGISQGSWVLFCFEKSRLAVVSMIAILKAGGVCVPIDPRYPVERIRDIIRTTNATIALVGAGKTAALFKSADTAVQTIDITKDIPHGLSDTVVQSNTKIDDPAFGLFTSGSTGVPKCIVVTHSQICTAVQAYKDRFGVTSETRVLQFSSYTFDISIADTFTALFYGGTLCIPSEEDRMSNLQDYMVSVRPNWAVLTPTVSRFLDPGVVKDFISTLIFTGEASREADTVPWIEAGVNLYNVYGPAENTLITTATRIRKGKSSNIGYGVNTRTWVTDVSGACLVPVGSIGELLIESGHLADKYLNRPDRTEAAFLSDLPWIPNYEGDSVRRGRRFYRTGDLVRYCDDGSLICVGRSDTQIKLAGQRVELGDVEAHLQSDPTTSQAAVVFPRSGPLEARLIALLVTGNKDGTPHNQQSLPKPAFAQCPPDLVKYATSSLQQRLPSYMVPSVWLGIDFLPMSVSGKLDRAVLQDQLESLSPSDYAEILGTTGLEVDPGGAASSVASDSDLRDMNDDSLLLTACSRVLNLPAGKISYSQSFIHAGGDSITAMQVSSWMKRFTGKRIGVKDLLVSPSISTAASCIKSAQDGSRNFVAVRPGQRIPVSPIQKLFFQTAEASKSWNHYHQSFLFRIDQPIKPQTIEDAISLVMQRHPMLQARFERTEEGDWYQYIPIDVERRASVEVIGSLSTDDREAAMLRARQSIDLTEGPLIRCQLFNNNVDEASRLFFVVIHHAVVDLVSWRIIMEELEAHLATDSTPDRGEAYQESVPFLAWCQVQAEAVKDIPVDRTVPLIPKIPTADFGYWGLKHDENVYGNTVERKIPLGHSITEDLLYKCHDSLHTKTIDVLLAAVLVSFRRSFLDRPVPAVFNEGHGREPGGEDAVDLSRTVGWFTTISPVYVPEVSPGDILDVVRRVKDYRWATPNNGFDYFSTKYLTQSGIKLFEDHLPAEILFNYEGRYQAMESEQTVLKPESWHAGEASKDQDPGLRRFCLFEISTAVLPDGQLHLTCSWNKNMRHQGRIRLWLDTLLPAAIGEIVSSLALASPQLTLSDVELLRLYDYSSLDILKKSILSIPAVQTLDDLEGVYPGSPMQDALFLSQSKSQDGAYEVDFTWRVATSLQNSQPAVDIGCLVEAWKDTVALHAALRTVILESSLPATGILHQVVLRSHDPDIVILDVRDVTAAITILDSYPPPTEEGIALIKRPPHRLLICTTIEGSVLIKFQVNHLVFDGMSTDKIIQDLSKAYTCRHSNKLPDHSESKLHDGTYGNRPTKPPLAEFIRYIRDPQRKQDSINYWKNALRGATTCSFPPLFDQITSEKAMPRQSWASVPIPLCVDSKELSKTLANLGITMSTMFQTVWAIVLRIYSQNGQSVFGYLTSGRDAPVDGIDSAVGNFIAMLVCFFDFDDDGVHTVADMARKIHNASANSISHQACSLAEIQDALGLSTSTPLFNTAFTYLPKRPTNVKAGEPEHHLCFEELSMSDPTEFDLTLFVEPTQESNEVSAHLDFKLSYISQAYATSIASTVAHILSELVHDPYRALNTLPIVSEHDTAIIRSWNDHLFPPATECIHETFSRKVVEHPQREAICSWDGSLTYAELSDLSQRLSIHLVSLGIKVGTKIPICFEKSMWTIVTILAVVQAGGVFVLLEPGHPESRLSGIIKQVQAELLLCSPATSRMGALQNISTQMGTEFKIVELEPEFIRSLPLPPKPNHQPMVGLNDDLYVVFTSGSTGVPKGAVATHQAYATGIYEHAVACGMTSLGAPPRSLQFASYSFDASIGDIFTTLAVGGCLCIPREEDRNPAGITTFINRYGVTWAGITPSLALHLDPDAVPTLKALCVAGEPLSMSVVTVWSKRLNLINMYGPTEATVACIANQVTCTTTTVSDIGRGYRATTWVVQPDNHNSLVPIGAVGELIIEGSILCRGYLNDPERTAEVFIRSPSWLHDLRPNSTLYKTGDLVRYSADGKIIFIGRKDTQVKMNGQRFELGEVEHALQLQLDPSDGPIIVDLLKRTQSGEPDLLIAFLFVGRANTGTGNSDEIFIATSTSSLSEFSTVIKKLQDAQRAMEVLPLFMVPQAYIPIEGGIPLTAAGKIDRRMLRKLCEPFNRNDLISFTSKALSTSVKDAETTDTVEDRLARIWEKVLGVKGVGRESDFFSSGGNSMAAIALRAEAQRSGFTLFVADIFTNPRLADMAKLFSHGQSVSPSSSTLRTKVPISSLQKRSSGLQTAAPVSNGSPVRRCQKENIIDCPVAFEYEEGPSDTQLKEASRICGISSRSIEDVFPCTPMQEALVALSLIPGAQASYALHAAFELRPGLDRNRFRSAWESTVKAQPILRSRIISGSNGSSVVVTSATDSIPQLDVSGLDTFLEQQLQVGFAPGAPLFRLAFVYSKADDCDYFVISAHHAIYDGWSLNLIWSQVLALYTNGELPPPGPSFKHFARNLNLVQSKLDSEDFWRKLLVKPDQESFRFPDVPVGHKPATRCTTNFHFPFSMQSKIGTTANTCINAAWAITLAQYSSNKTVNFGVTLWGRDFPMIDIEHMTGPTIVTVPRQVNVIPESSVAEFLQDLQKSLAVVLPHQHLGLHRIQALGPIARQACDFSTLLVVNHGSSISWSELEAADIVPVPLRSSDLYAYPMVVEVENASSDTLDIRVHSDPDCIEVQLLERLMEQFGHNLQTLCRAASFDPGKRIAELMDDTATTHLRTLFSWNSRVKDSPDVAAIAVHKLLEETAQSQPAESAIVAHDGQLSYMQMDRCADVLARQIRKTNMISAQSPFVCIHLLRSATAVVSMLAVLKAGGAFMPVDISQPRSRLQNLIEESGAKLVLTLPESANALATLSGLTKVIPVSLSELVQQITDNTTKKDEYCKSGDTDPSSPAYLLYTSGTSGKPKGVVMEHRAWSLGFTCHAEYMGFNSCTRILQFSSLMFDLSILEIWAVLYAGGCLFIPSDKERVNNLQDFTRINDINTVFLTPSIGKLLNPKDLPNISFAGFIGEPMTRSLIDAWTLPGRRLVNSYGPTEACVLVTAREISPTAPHDKPSSNIGHALGANIWVVEPQRTALVPIGAVGELCIEAPSLARCYLANPERTEYSFPSTVLDNWQTKKGTRVYRTGDLVRYASDGTLDFLGRKDGQIKLRGQRIELGEIEHHIRRLMSDDPRFHEASVQLYNPATDPDRDATVDVQMREPYLAGLLVLDLVFTDEVMGIPCTSLTSANTSENLQTLVTELKKSLRGVLPHYMVPLHFVAVSRLPTGSSGKLDHAFVRACLRELTAPLDGNFPKVEQVLTTNESVLRQWWGTVLAMDPHSIQRGDDFFSLGGSSISAMRLVGLARSSGHKLQHEDIFMCPRLADMAGQISFVQEASVSPTTSPTIKFDLLDDCEVDEVIDHILPQLDMNKELIEDVYPCTPLQESLMAATARHGEAYTMIQSITVLASQLAQLKKAMDVVFRDFEVLRTRIALGPSQQALQVVVKHEELSWESFPSIQSFKDHFYRSLGYGKPLARLAVITQALDTKQPISHGTREARTKNSQDTVMVVVGAHHSIYDAHVLSMIWRRLYREFIGSQADGILEAETSRSEGVVPFKSYVEKLLRGKDNDESLLFWKEKLRGVSSSQFPPASWPRVLEHQPSATQTLITKVSLPTSSRKKLGATVATVAYAAWALTIAHYTADPDVVFGATLSGRETMAGSISHPESIAGPTIITVPLRIIIDFQTVVSDFLSTLQKDIVRAAYFGQMMGLNSIAHIDNDCRDACGFKSIIVVQVPDEGENHDGRAANPFQMSLESIGHFPAPLVVEVEQSESTDVLIRMAYDPVLVPEKLAHFISDTFTTTMSNLSAANPKAKVESIPALSEAHLAELDVTCPEWILGKAKDEKIRTESHQCLQDLVCRRAQQSPNSQAIDSWDGSISYHELDGLSSILAEHLSQLGVRPEAPVCLLFEKSKWAVVAMIGIIKAGGCFVPLDPSYPHERLEHIISETGSSVIVTSAAYSKLCLSLSVRGIVCDGSVFSSTKKPLPSTADSPPSFSVRPNQAAYILFTSGSTGKPKGVVMEHHSVCSALIALGKRMGLGPQSRVLQFNSYWFDVMLLDIFGTLVYGGCLCIPKEEQRMSNLSGWVQKFKVNTMLLSTSVSRLMQPADTPSLETLCLTGEAVLQSDVDRWAPKLHLIAGYGPTETCIMSVSGELTPSSPANLIGKPVSCQAWVINPLKETELAPYGATGELYIQGPTVARGYLHDDVLTSKAFIVDPQWLTGYKTNENQWSRRAYKTGDLVFWGPQSNLYYVRRKDSSQVKIRGQRVELAEIEEVIRQHIPPDVTVCVDLLSSDDQNTRIILGAVLGIGDRALGGPEDLEVIGYMDDLKSHIIPALEASLPHHMIPEAYVPFVQLPTLGSGKLDRKTVRRVAGPLAFSLPQASARHPNQPTVTHTQKLLRQLWCKILPQLDESAVNKQDNFLGIGGDSIAAIKLVALLRQHGISLAVAEIFTRPTLEAMSSLIDEHNFVVSHAGILSDVTRNTSGVMRQTTNLIAGRHSMAVEKSRECDNSTLPCTEYQQMFLAGTEAFTGAHSAQFIFRLPEKIDLDRLQAAFDHCADWYPNLRTQIHKDADTGRLLHDISPIGVKVPWSCHYSDDLNTVLSHDKKFPPGLDGPLHRVTIMRHRDPTESMLVWTLNHAAYDAWSLRMMLEHITEAYANPDYEPSYSLGWTAFVLHTENTKEASRSFWSSYLSDVKPARLMFNYNLVSNPRQDRLYEARINIPKRVLSQATAATVLLAGLTLLVARVCDTRDVILAHLLTGRTLPLAGIENCPGPTITKVPLRIPLMDQDLVTLELDSVAKKITAELMRVMPHEHSGLSAIREFIPQAEGTTTSSGKFHAGSVLGRLPLDLVIHPKGGLDLLGKHGLGLQNEGFRLVAPPSGGLSMECALVDDDDDKRSDTISVDVSVLWDQRAATQEDVIELVHSLQGIFTKRNLAASICLMYK