Function
Highly reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of 10,11-dehydrocurvularin, a prevalent fungal phytotoxin with heat shock response and immune-modulatory activities (PubMed:26493380). The highly reducing polyketide synthase Dhc3 is responsible for biosynthesis up to the tetraketide stage (PubMed:26493380). The non-reducing polyketide synthase Dhc5 then conducts four additional chain extension cycles, producing the unreduced part of the nascent octaketide from C-1 to C-8 in 10,11-dehydrocurvularin (PubMed:26493380).
Sequence
MKTGHRTVLLFGDVTDPWIEGIDHVYVQAAKKPWIRSFLEDLFSAIKTETKAMDRVLQEGFKDSSSFQELAERYRHAGDDFGMAHAMMIYAIRAVVLLETLSREPHILDESRPRPEVIGISGGLFSAAVMSISINFETLYAACIEAGRVWSRLCNLTLVRSRAMEESPGTWGWAILGIPAADLGKTLEQFQSSMGIHCSKRAKVGVIGDRWSTVIGPPSVLELTLSECPNLKNLPKDELNIHALQHTLSVSNSDIDYIVGNSPLLETPLPPGYRIHGLDDDFPEANYAEWRHLLRASAYQTLSRPLDIVQAVSNLNAALGVSKDIDAKIIGPSSHTTYLVKCLQTAGKEVLIQNGFPASPQTSGTNDGIAIVGMAGKGPGSDDLEEFWNVILKGLDLHEEVPSDRFNLEEYYSPKHPPAGPGRCTMTCRHGCFMNNPGHFDSKFFHISPREAMLMDPAHRLFLMNAYEALEMAGYSNGQTKSTNPTKIATFFGQCNDDWHVVGHRALGCDAYTLQAVQRAFGPGRLAFQFNWEGPTYALDSACAATSSCIHLACMSLITRDIDMAVAGAANVLSTPHSFTSLSRSGVLSDSGNCKTYRDDADGYCRADFSGAVVLKRLDDAIAQNDNILAVISSSARNHSGNSTSITTSDAAAQERLFQKVLRNARVTPQDISYVEMHGTGTQVGDKAEIGAVSSVFSKRIDGRPLNVGAIKANIGHSEAAAGMSSLLKSILMFQNSTIPPQAGMPHTLNPNFPPLHEINIQIPSEPLEFKTTDNKPRRILLNNFDAAGGNACLLLEDYTDTKERNADVRSAHTIVMSARTQSSQLLNKKRLLRWLRSKPNTRVEDLAYTTTARRMHHPIRSAIIASTTQEVIAKLEAEIERNDSSLVQRASPLVFVFTGQGSHYGGMGSELYGSSPIFRERVDLCASICAGYDFPPFLDIITNETVNVSTKNASQVQLAVLTLEMALTHFWRSAGIEPTMVIGHSLGEYAALHAAGVLSLADALYLVGHRSLLLLERCESDSCSMLSVSMSVDEVQAQLTRIRSSCNVACINSSTSTVVSGTAEDLAEFQSSITAQNAKVRAKKLSLPFAFHSFQMDPILEDYSRIAAGVTYSAPKIPVASTLLGSVVNREGIFTQEYMVQQTRQAADFMGGLVAVKSELADPLWLEVGPAPVCMSFVRDTLSTPASKMTHSLQPKTSNWMSLSKTLAAMYTSGVDIDWLAFHAPYESDLKLLTLPSYAWDVRNFWITHTDNATEVVSEQSPVTSSEPLISTCAQYLVAKSSSPNIRVALRASIADPGFMGLMDGHKMQGIGLCSGSVFCEAAFAATKYALEYSGRKNITQPWLTLHDPKLLLPLTKKSVGPDGDLVTTAVMYSSSAETISITFQVTSASASYDLGTCVVKVRDPTRSQVEWDRISYFIKARMDETIKNAKEGRGHRMQPEVFYALFGNAVEFAPDFQGINEAYIAKDFQEAAAVVTLPHDPSGTRFTFSPYWGEALVHLAGFMVNGNPSKSPKTTFIVMGFASVEQTAPLIPGKQYMTYTRISKWVKDTAYCDAIVFDPESFTIVLQCVDIRYQELPRATWKHVLEGPHETPIVHAQRPPVRNSKKYVETRELQQPSSATVPAQETTIDEPEQQEGEPAAGARLFNAILDSISKATGTDPSEFRDDTMIADLGVDSIMAIEVVATVKDVSGLELPAAFVFEYPTIGDLRKEFRANEPTVENPRFSATPSSAEASIPSSPSSLAHPMSDSASSLSPSDREEALPLERQSMTKREQKRPVKIDDDASPEPVVRIMLLQGRPGSKRIPFYMMADGTGTIATYIHLPPFKSKMPVYGIDSPFLRCPKRLTKEVGIEGVAKLIVDALMKTQPEGPLMIGGFSAGSIVAYEVCRQLGRAGRQVEGLVLIDMCCPRSSLLDEDKMNSEDDASFAIFESAVTKDGLWSSSETTQQHFRAYHVAMHAYHPPYMLEEERPTRTAVIWAEKGMVNRVMGNEKLMKMLVEQGIPMTSYPGYMEDPKLGAFACLVPDRTKADLGPNGWEKYTAGEVLTLSVAGDHLDLPMPGHVHLLHAQMEKAFAYIKG