DNA2

DNA replication ATP-dependent helicase/nuclease DNA2

1522 amino acids

Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is atypical: it cannot load onto its tracking strand internally and has an absolute free 5'-end requirement. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

Belongs to the DNA2/NAM7 helicase family.

171.694 kDa

MPGTPQKNKRSASISVSPAKKTEEKEIIQNDSKAILSKQTKRKKKYAFAPINNLNGKNTKVSNASVLKSIAVSQVRNTSRTKDINKAVSKSVKQLPNSQVKPKREMSNLSRHHDFTQDEDGPMEEVIWKYSPLQRDMSDKTTSAAEYSDDYEDVQNPSSTPIVPNRLKTVLSFTNIQVPNADVNQLIQENGNEQVRPKPAEISTRESLRNIDDILDDIEGDLTIKPTITKFSDLPSSPIKAPNVEKKAEVNAEEVDKMDSTGDSNDGDDSLIDILTQKYVEKRKSESQITIQGNTNQKSGAQESCGKNDNTKSRGEIEDHENVDNQAKTGNAFYENEEDSNCQRIKKNEKIEYNSSDEFSDDSLIELLNETQTQVEPNTIEQDLDKVEKMVSDDLRIATDSTLSAYALRAKSGAPRDGVVRLVIVSLRSVELPKIGTQKILECIDGKGEQSSVVVRHPWVYLEFEVGDVIHIIEGKNIENKRLLSDDKNPKTQLANDNLLVLNPDVLFSATSVGSSVGCLRRSILQMQFQDPRGEPSLVMTLGNIVHELLQDSIKYKLSHNKISMEIIIQKLDSLLETYSFSIIICNEEIQYVKELVMKEHAENILYFVNKFVSKSNYGCYTSISGTRRTQPISISNVIDIEENIWSPIYGLKGFLDATVEANVENNKKHIVPLEVKTGKSRSVSYEVQGLIYTLLLNDRYEIPIEFFLLYFTRDKNMTKFPSVLHSIKHILMSRNRMSMNFKHQLQEVFGQAQSRFELPPLLRDSSCDSCFIKESCMVLNKLLEDGTPEESGLVEGEFEILTNHLSQNLANYKEFFTKYNDLITKEESSITCVNKELFLLDGSTRESRSGRCLSGLVVSEVVEHEKTEGAYIYCFSRRRNDNNSQSMLSSQIAANDFVIISDEEGHFCLCQGRVQFINPAKIGISVKRKLLNNRLLDKEKGVTTIQSVVESELEQSSLIATQNLVTYRIDKNDIQQSLSLARFNLLSLFLPAVSPGVDIVDERSKLCRKTKRSDGGNEILRSLLVDNRAPKFRDANDDPVIPYKLSKDTTLNLNQKEAIDKVMRAEDYALILGMPGTGKTTVIAEIIKILVSEGKRVLLTSYTHSAVDNILIKLRNTNISIMRLGMKHKVHPDTQKYVPNYASVKSYNDYLSKINSTSVVATTCLGINDILFTLNEKDFDYVILDEASQISMPVALGPLRYGNRFIMVGDHYQLPPLVKNDAARLGGLEESLFKTFCEKHPESVAELTLQYRMCGDIVTLSNFLIYDNKLKCGNNEVFAQSLELPMPEALSRYRNESANSKQWLEDILEPTRKVVFLNYDNCPDIIEQSEKDNITNHGEAELTLQCVEGMLLSGVPCEDIGVMTLYRAQLRLLKKIFNKNVYDGLEILTADQFQGRDKKCIIISMVRRNSQLNGGALLKELRRVNVAMTRAKSKLIIIGSKSTIGSVPEIKSFVNLLEERNWVYTMCKDALYKYKFPDRSNAIDEARKGCGKRTGAKPITSKSKFVSDKPIIKEILQEYES

dna2

DNA replication ATP-dependent helicase/nuclease dna2

1397 amino acids

Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape fen1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit dna2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for fen1. Is a target of the intra-S phase checkpoint, associating with stalled replication forks when phosphorylated at Ser-219 and preventing the stalled replication forks from reversing. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA. Also required for the production of G-rich single-strand overhangs at telomere ends and thus in telomere length maintenance. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is atypical: it cannot load onto its tracking strand internally and has an absolute free 5'-end requirement. Helicase activity may promote the motion of dna2 on the flap, helping the nuclease function.

Belongs to the DNA2/NAM7 helicase family.

157.659 kDa

MFNDQSKTTSSVKGICATTDNNHGNLKKTNSTPFRKNYLLNGRTKLKLENFAYNASTEISSPKISEKKHSSLPIKRKNTFNESSTSFSPFTKAHKEITDDLKPDKSFTRKSDLNSQDMPVCFQETSKDLCRSSSTQHLLDHQTTDSTIIDMKPVSTNSKSDVFTLYTDETVLLRRCASDNKPLINNNLSSSNVSENQSRSFGSYDEVKNQGNNLHKVPSLVSIIRNARSSEQSRIAANSSCLLKGSDTEIDEDDFALEAEDLAALDSLERQYSQLPNSTVTASAKDIEKTAKVNHVGGDLQSYCSATKASDATINEEPVNLALDKACNSLPDINSDFIDDWDDSCDGCTPGELCEFSSEYTVLEVHEDFIFHEGNHFRQLKLILEANDILHQLFLRGDWTETSIFVGDSIRVEATFDKDNTAIVDNDKGLIIIHPKILMSATAVASSFPCLRKAVISDRVGIYGPPTKAMVTGNILHDFFQHALYRGIDALENVDINLETSIKTYISDIYFADLSLDEIREELDARLPLLKSIVERYLISKKNDNNNESIHISRLLDIEESIWSPRFGLKGNIDATVEVVLTEKPESSSTLTLPLELKTGRYVDNISHFAQSLLYTLLISDRYGINTNQALLCYLENSTIKNLVASNSQLRGLIMTRNSLAQHNFRRSLPEMISNRKICDHCSLVSECLFFQKMSDKGVANSNGLTESWNEWMREVKDEDLEFYKKWEKLLNQEERLLLLKRGDVLTFDTEELEAYGKTLYPLYITKEDIVCLEIDDRVFHYKFAFLNDNGYPRNFLHSGFSVGERVFISDEHGHWSLAKGHIVHIQDSCIEVRTRHRLHIPWLKMPNFDFKKNQVFFGNYEDSKLSFIGSNHTRYRIDKDEFSSGIASIRGTLMSSVLPDAPLIIRDMIIRLKPPKFCNSALIDPEFLKCLNEDQITALKKCHAAEHYSLILGMPGTGKTTTISSLIRSLLAKKKKILLTSFTHLAVDNILIKLKGCDSTIVRLGSPHKIHPLVKEFCLTEGTTFDDLASLKHFYEDPQIVACSSLGVYHSIFNKRKFDYCIIDEASQIPLPICLGPLQLAEKFVLVGDHYQLPPLVKNSRTSKDGLSLSLFKLLSEKHPEAVTTLRLQYRMNEDINSLSSELIYGGNLVCGSKTISQKKLILPKAHLSDGLPDSSSSLHWVNKLINPSHSVIFFNTDDILGVESKTNNILENHTEAFLIEQAVSSFLERGVKQSSIGIISIYKSQVELLSKNLKSFTEIEINTVDRYQGRDKDIILISFVRSNSKNLVGELLRDWHRLNVALSRAKVKCIMFGSLSTLSSSNIVSHLLKLLEKNKWIFTLNENDIATKFDENSSPIKDCSQVATTNNAKVIIRKNQRFFNSDNLCEKAILPQLEF

Dna2

DNA replication ATP-dependent helicase/nuclease DNA2

1062 amino acids

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function (By similarity).

Belongs to the DNA2/NAM7 helicase family.

119.447 kDa

MEPLDELDLLLLEEDGGAEAVPRVELLRKKADALFPETVLSRGVDNRYLVLAVETSQNERGAEEKRLHVTASQDREHEVLCILRNGWSSVPVEPGDIVHLEGDCTSEPWIIDDDFGYFILYPDMMISGTSVASSIRCLRRAVLSETFRGSDPATRQMLIGTILHEVFQKAISESFAPERLQELALQTLREVRHLKEMYRLNLSQDEILCEVEEYLPSFSKWAEDFMRKGPSSEFPQMQLSLPSDGSNRSSPCNIEVVKSLDIEESIWSPRFGLKGKIDVTVGVKIHRDCKMKYKVMPLELKTGKESNSIEHRSQVVLYTLLSQERREDPEAGWLLYLKTGQMYPVPANHLDKRELLKLRNWLAASLLHRVSRAAPGEEARLSALPQIIEEEKTCKYCSQIGNCALYSRAVEEQGDDASIPEAMLSKIQEETRHLQLAHLKYFSLWCLMLTLESQSKDNRKTHQSIWLTPASELEESGNCVGNLVRTEPVSRVCDGQYLHNFQRKNGPMPATNLMAGDRIILSGEERKLFALSKGYVKKMNKAAVTCLLDRNLSTLPATTVFRLDREERHGDISTPLGNLSKLMESTDPSKRLRELIIDFREPQFIAYLSSVLPHDAKDTVANILKGLNKPQRQAMKRVLLSKDYTLIVGMPGTGKTTTICALVRILSACGFSVLLTSYTHSAVDNILLKLAKFKVGFLRLGQSHKVHPDIQKFTEEEICRSRSIASLAHLEELYNSHPIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLFFSRRFVLVGDHQQLPPLVVNREARALGMSESLFKRLERNESAVVQLTVQYRMNRKIMSLSNKLTYAGKLECGSDRVANAVLALPNLKDARLSLQLYADYSDSPWLAGVLEPDNPVCFLNTDKVPAPEQVENGGVSNVTEARLIVFLTSTFIKAGCSPSDIGVIAPYRQQLRIISDLLARSSVGMVEVNTVDKYQGRDKSLILVSFVRSNEDGTLGELLKDWRRLNVALTRAKHKLILLGSVSSLKRFPPLGTLFDHLNAEQLILDLPSREHESLSHILGDCQRD

DNA2

DNA replication ATP-dependent helicase/nuclease DNA2

1061 amino acids

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function (By similarity).

Belongs to the DNA2/NAM7 helicase family.

120.485 kDa

MERVDELELLMEKSFWQEAEPSAELFQKKKVEASFSKIVLSRGMDNRYLVLAVDIVQSEEGNHEKHLIITASQSLEYKELCILRNDWCSVPVEPGDIIHLEGDCISNTWIIDEDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRSSDPATRQMLIGTVLHEVFQKAVSDSFAPEKLQELASQTIQEIRHLKEMYRLKLNQDEIKQEVEEYLPSFSKWAGDFMHKHTSTDFPQMQLSLPSDGSNSNSTCNIEVTNSLDIEESIWSPRFGLKGKIDVTVGVKIHRGCKTKYKIMPLELKTGKESNSIEHRSQLVLYTLLSQERRADPEAGLLLYLKTGQMYPVPAKHLDKRELLRLRNQMAFSLFHRINKSTGEKTELAPLPQIIEEQQTCKYCSQMGNCALYSRAVEQQMEDSSVPTSMWPKIKEETQHLKPIHLEYFSLWCLMLTLESQSKDNKRNYQHIWLMPASEMEESGSCIGSLIRIEHVKTVCDGQYLHNFQRKNGAIPITNLMAGDRIILSGEERTLFALSRGYVKEINSTTVTCSLDRNLSGLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTRASQKLRDLIIDFREPQFISYLSSVLPHEAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPDIQKFTEEEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKNAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLPNFKDVKLELEFYADYSENPWLIAAFEPNNPVCFLNTHKVPAPEQVEKGGVSNIMEAKLVVFLTSVFIKAGCKPSDIGIIAPYRQQLKVISDLLAQSSVGMVEVNTVDRYQGRDKSIVVVSFVRSNEDGTLGELLKDWRRLNVAITRAKHKLILLGCVPSLSRYPPLRKLLNHLNSEKLIIDLPSGEHESLFHLLGDFQRK

DNA2

DNA replication ATP-dependent helicase/nuclease DNA2

1060 amino acids

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

Belongs to the DNA2/NAM7 helicase family.

120.415 kDa

MEQLNELELLMEKSFWEEAELPAELFQKKVVASFPRTVLSTGMDNRYLVLAVNTVQNKEGNCEKRLVITASQSLENKELCILRNDWCSVPVEPGDIIHLEGDCTSDTWIIDKDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRSSDPATRQMLIGTVLHEVFQKAINNSFAPEKLQELAFQTIQEIRHLKEMYRLNLSQDEIKQEVEDYLPSFCKWAGDFMHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVVKPMDIEESIWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRADPEAGLLLYLKTGQMYPVPANHLDKRELLKLRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNHQNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKIFDFCIVDEASQISQPICLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKSAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKVPAPEQVEKGGVSNVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGMVEVNTVDKYQGRDKSIVLVSFVRSNKDGTVGELLKDWRRLNVAITRAKHKLILLGCVPSLNCYPPLEKLLNHLNSEKLIIDLPSREHESLCHILGDFQRE

Dna2

DNA replication ATP-dependent helicase/nuclease DNA2

1059 amino acids

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function (By similarity).

Belongs to the DNA2/NAM7 helicase family.

119.588 kDa

MEPLDDLDLLLLEEDSGAEAVPRMEILQKKADAFFAETVLSRGVDNRYLVLAVETKLNERGAEEKHLLITVSQEGEQEVLCILRNGWSSVPVEPGDIIHIEGDCTSEPWIVDDDFGYFILSPDMLISGTSVASSIRCLRRAVLSETFRVSDTATRQMLIGTILHEVFQKAISESFAPEKLQELALQTLREVRHLKEMYRLNLSQDEVRCEVEEYLPSFSKWADEFMHKGTKAEFPQMHLSLPSDSSDRSSPCNIEVVKSLDIEESIWSPRFGLKGKIDVTVGVKIHRDCKTKYKIMPLELKTGKESNSIEHRGQVILYTLLSQERREDPEAGWLLYLKTGQMYPVPANHLDKRELLKLRNQLAFSLLHRVSRAAAGEEARLLALPQIIEEEKTCKYCSQMGNCALYSRAVEQVHDTSIPEGMRSKIQEGTQHLTRAHLKYFSLWCLMLTLESQSKDTKKSHQSIWLTPASKLEESGNCIGSLVRTEPVKRVCDGHYLHNFQRKNGPMPATNLMAGDRIILSGEERKLFALSKGYVKRIDTAAVTCLLDRNLSTLPETTLFRLDREEKHGDINTPLGNLSKLMENTDSSKRLRELIIDFKEPQFIAYLSSVLPHDAKDTVANILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICALVRILSACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQSHKVHPDIQKFTEEEMCRLRSIASLAHLEELYNSHPVVATTCMGISHPMFSRKTFDFCIVDEASQISQPICLGPLFFSRRFVLVGDHKQLPPLVLNREARALGMSESLFKRLERNESAVVQLTIQYRMNRKIMSLSNKLTYEGKLECGSDRVANAVITLPNLKDVRLEFYADYSDNPWLAGVFEPDNPVCFLNTDKVPAPEQIENGGVSNVTEARLIVFLTSTFIKAGCSPSDIGIIAPYRQQLRTITDLLARSSVGMVEVNTVDKYQGRDKSLILVSFVRSNEDGTLGELLKDWRRLNVAITRAKHKLILLGSVSSLKRFPPLEKLFDHLNAEQLISNLPSREHESLYHILGDCQRD

dna2

DNA replication ATP-dependent helicase/nuclease DNA2

1053 amino acids

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape fen1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit dna2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing (By similarity). Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of dna2 on the flap, helping the nuclease function.

Belongs to the DNA2/NAM7 helicase family.

119.732 kDa

MEPVSAECHLPPEDDLLEMMMEQSFTEPEEKSQDKPTRKIIPKTKLCKGVNNRYCVLHIKEVYAQREEKHLTITASQEGDDLELCILKDDWVALQIKPGDIIHLEGNCSVDNTWTISRDTGYLILYPDLLISGTSIANGIRCLRRSVLSEKFKVCDKGSRQMLNGTMLHDIFQRATTCGFTDSVLQELAHHTVHGPKYLKEMYQLKLNQADVMGEIQEYLPSLSKWATDFMTHPLNQQQINRTKSTAGDPTETTKVSEFLDIEENIWSPRFGLKGKIDVTARVKIHQKSKAHLKIMPLELKTGKESNSIEHRSQVVLYTLLSQERREDPEAGLLLYLKTGNMYTVPGNRLDRRELLKIRNELSYYLTNVLHKSDNGSKETTLASLPAMIADRQACKYCSQMRNCALYNRSVEQQTENCYIPPEMIPVVQKETEHLTEDHLQYFRLWYLMCTLEANSKDSKMGRKNIWMMSSSEREEDGQCIGNLIRTGHVQTISDVQYLHSFQRRSGSVPATNLASGDRVVVSGEERFLALSTGYIKEVKDENITCILDRSLVKLPEDLLFRLDHEEGGGGLEFHLGNLSRLMENSSVSEKLRKLIIDFSKPNFVQHLSSILPPDAKDIVASILRGLNKPQKQAMKRVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLKKFQVGFLRLGRTQKLHPDVQEFSEEEICKAKSIKSLSALEELYNSQPVVATTCMGVNHPIFTRRRFDFCIVDEASQISQPICLGPLFFADRFVLVGDHQQLPPLVKSAEARELGMSESLFKRLERNQEAVVQLTVQYRMNSKIMALSNKLVYEGRLECASDRVSNAVVQLPHIKTLLLELEFRESQESMWIKDVLEPSNPVCFLNTEKIPALETEEKGGISNWIEAKLVFHLTKLYLKAGCRPSDIGIIAPYRQQLKMISNYFNSLSASAVEVNTVDKYQGRDKSVIIVSFVRSNIDGKLGDLLKDWRRLNVALTRAKHKLIMLGCVPTLNRFDCLEQLICNLKTENQIYDLPEGAHEHFPV

dna2

DNA replication ATP-dependent helicase/nuclease DNA2

1048 amino acids

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape fen1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit dna2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of dna2 on the flap, helping the nuclease function (By similarity).

Belongs to the DNA2/NAM7 helicase family.

118.558 kDa

MEPVSAECQLPPEDDLLEMMMEQSFTEPEEKSKDKSTRKIIPKTKLCRGLNNRYCVLNVKEVYAQGEEKHLTITASQEGDDLELCILKDDWVALHIKPGDIIHLEGNCAFDNTWTISRDTGYLILYPDLLISGTSIANGIRCLRRSVLSEKFKVCDKGSRQMLIGTMLHDIFQRATTRGFTDSVLQELAHHTVHGPKYLKEMYQLKLNQTDVMGEVQEYLPSFAKWAIDFMTHPLNQHQINVTRPTAGDPTEATKVSEFLDIEENIWSPRFGLKGKIDVTARVKIHQKSKSHLKIMPLELKTGKESNSIEHRSQVVLYTLLSQERREDPEAGLLLYLKTGNMYSVPGNRLDRRELLKIRNELSYYLTNVVHKSDNGSKEITLASLPALIADRQACKFCSQMRNCALYSRSVEQQIENCYIPAEMIPVVQKETEHLNKDHLQYFRLWYLMCTLEGNSKDSKMGRKNIWMMSSSEREEDGQCIGNLIRTGPVQTISDGQHLHSFQRKSGTVPATNLMSGDRVVVSGEDKFLALSSGYIKEVKHNNITCILDRSLGKLPEDLLFRLDHEEGGGGLESHLGNLSRLMENSPVSDKLRKLIIDFSKPNFVQHLSSVLPSDAKDIVANILKGLNKPQKQAMKRVLLSKDYTLIVGMPGTGKTTTICTLVSTLHLKTCTSCLVDDHREAAGTHRSHRHSKGTVNLGKQKRQHRVTSTTSSTSAKCRSKTEPAVSAENKKLQKLISGSHVCIMVNGHKTFSQNNYIIFFDNILLLKKLPLHYMCAFALIHHQCFKGCVKSVSLVRELGMSESLFKRLERNQEAVVQLTVQYRMNSQIMALSNKLVYEGRLECASDRVSNAVVKLPHIKTLLLELEFRESQESMWIKDVLEPSNPVCFLNTEKIPALETEEKGGISNWIEAKLVFCLTKLFLKAGCRPSDIGIIAPYRQQLKVISNYFNSLSASAVEVNTVDKYQGRDKSVIIVSFVRSNIDGKLGDLLKDWRRLNVALTRAKHKLIMLGCVPTLSRFLCLEQLICHLKSKNHIYDLPAGAHEHMPV

DNA2

DNA replication ATP-dependent helicase/nuclease DNA2

992 amino acids

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function (By similarity).

Belongs to the DNA2/NAM7 helicase family.

111.679 kDa

MADPSNAALRSGLNNRYRVLEVRVVRGEGRDPEKHLAVSSDPSLGDTELCVLQNGWESVPVVPGDIVHLEGDCSSGTWVINEQSGYLILYPDLLLSGTTISSSIRCMRKAVLSERFRGSECGSRQTLVGTILHEIFQQSVTNNLSPEKVEELAKKIVYGQKYLKEMYHLKLKQTEIMQEIEEYLPSFFKWTEDFVRNPANQNKMQLKLPSENKTGDCSSTVEIVDILDIEENIWSPRFGLKGKIDVTARVKIHRQGGIQSRIMPLELKSGKESNSVEHRSQVILYTLLNLERRVDPEAGFLLYLKTGTMYPVTGTRMDRRELIKLRNQVAFYLMHSTYKSAVGRQQSQLAALPPLIDDSQACKYCSQIHNCFLYSRAVEERMASVSFPPALIPIIEKETQHLKPSHLEYFSLWYLMLTLEMQSGDSKKGYKNIWMIPSLEREKAGDCVGNMIRVDQVQEVSEGQYLHSFQRKNGAVPGANLLVGDRVVVSGEENGLLGLATGYVREISATKISCLLGRNLSKLPESTTFRLDHEEGDCSIGVPFENLSKLMKDSPVSEKLRNLIIDFHKPRFIQHLSSVLPPEAKEAVASILKGLNKPQKQAMKQVLLSRDYTLIVGMPGTGKTTTICALVRILSACGFSVLLTSFTHTAVDNILLKLAKFKVGFLRLGRAQKVHPDIRKFTEEEICRSKSIKSVTDLEELYNSQPVVATACMGINHPIFVQKQFDFCIVDEASQISQPICLGPLFCSKRFVLVGDHQQLPPLVQNSEARDLGMSESLFKRLEQNQNAVVQLTVQYRMNSKIMSLSNKLVYEGKLECGSEKVSNATANLPNLKMLKLEFADASKTWLKEVLEPDKPVCFLNTEKAGCRPSDIGIISPYRHQLKVITDLMARLKENRVEVNTIDKYQGRDKSIIIVSFVRNSNDENLGALLKDWRRLNVAITRAKHKLIMVGCVPSLRRYPPLEKLLCHLQSEAMIFNLPPGAHESIHKCNIL